关节镜治疗髋关节撞击综合征的短期临床疗效

目的探讨关节镜治疗髋关节撞击综合征(FAI)的短期临床疗效。方法选取该院2015年8月-2017年8月行髋关节镜治疗的FAI患者30例。其中,男18例,女12例;年龄26~59岁,平均33.85岁。术前髋关节活动度评估:外展(30.75±6.74)°,髋关节屈曲90°位内旋(14.84±8.22)°,屈曲(95.25±13.13)°,术前髋关节Harris评分(32.55±5.62)分,术前视觉模拟评分(VAS)(7.24±2.12)分。结果 30例患者术后均未出现感染,切口Ⅰ级/甲级愈合,无神经和血管损伤,所有患者均获得12~18个月的随访,术后关节疼痛明显改善,基本恢复至健侧关节水平,VAS评分降至(0.76±0.65)分,髋关节屈曲90°位内旋活动度恢复至(46.56±7.62)°,髋关节Harris评分增加到(96.24±3.64)分。结论关节镜治疗FAI短期临床疗效满意。

The correlation of the quantitative ultrasonography parameters with the liver cancer cell proliferation activity and intralesional angiogenesis

Objective:To study the correlation of the quantitative ultrasonography parameters with liver cancer cell proliferation activity and intralesional angiogenesis.Methods: A total of 46 patients with liver cancer who received surgical resection in Hancheng People's Hospital between June 2014 and March 2017 as well as 28 patients who received partial hepatectomy for abdominal trauma during the same period were selected, the patients with liver cancer received ultrasonography, and the quantitative parameters were calculated;the liver cancer tissue and adjacent tissue of patients with liver cancer as well as the normal liver tissue of patients with abdominal trauma were collected to determine the expression of proliferation genes, tumor suppressor genes and angiogenesis genes.Results:The IMAX of liver cancer tissue was significantly higher than that of adjacent tissue while the TTP, WT and mTT were significantly shorter than those of adjacent tissue;RNF180, Merlin, PICK1 and PTEN protein expression in liver cancer tissue and adjacent tissue were significantly lower than those in normal liver tissue, negatively correlated with the IMAX of liver cancer tissue, and positively correlated with the TTP, WT and mTT of liver cancer tissue;EphB4, SALL4, Cripto-1, VEGF, PDGF, EGFR and Ang-2 protein expression in liver cancer tissue and adjacent tissue were significantly higher than those in normal liver tissue, positively correlated with the IMAX of liver cancer tissue, and negatively correlated with the TTP, WT and mTT of liver cancer tissue. Conclusion: The changes in the quantitative parameters of liver cancer ultrasonography are valuable for evaluating the cell proliferation and angiogenesis.

Lung macrophages are involved in lung injury secondary to repetitive diving

This study aimed to establish an animal model of decompression-induced lung injury(DILI)secondary to repetitive diving in mice and explore the role of macrophages in DILI and the protective effects of high-concentration hydrogen(HCH)on DILI.Mice were divided into three groups:control group,DILI group,and HCH group.Mice were exposed to hyperbaric air at 600 k Pa for 60 min once daily for consecutive 3 d and then experienced decompression.In HCH group,mice were administered with HCH(66.7%hydrogen and 33.3%oxygen)for 60 min after each hyperbaric exposure.Pulmonary function tests were done 6 h after decompression;the blood was harvested for cell counting;the lung tissues were harvested for the detection of inflammatory cytokines,hematoxylin and eosin(HE)staining,and immunohistochemistry;western blotting and polymerase chain reaction(PCR)were done for the detection of markers for M1 and M2 macrophages.Our results showed that bubbles formed after decompression and repeated hyperbaric exposures significantly reduced the total lung volume and functional residual volume.Moreover,repetitive diving dramatically increased proinflammatory factors and increased the markers of both M1 and M2 macrophages.HCH inhalation improved lung function to a certain extent,and significantly reduced the pro-inflammatory factors.These effects were related to the reduction of M1 macrophages as well as the increase in M2 macrophages.This study indicates that repetitive diving damages lung function and activates lung macrophages,resulting in lung inflammation.HCH inhalation after each diving may be a promising strategy for the prevention of DILI.

Phytochemical interventions for post-traumatic stress disorder:A cluster co-occurrence network analysis using CiteSpace

Objective:This study investigated trends in the study of phytochemical treatment of post-traumatic stress disorder(PTSD).Methods:The Web of Science database(2007-2022)was searched using the search terms“phytochemicals”and“PTSD,”and relevant literature was compiled.Network clustering co-occurrence analysis and qualitative narrative review were conducted.Results:Three hundred and one articles were included in the analysis of published research,which has surged since 2015 with nearly half of all relevant articles coming from North America.The category is dominated by neuroscience and neurology,with two journals,Addictive Behaviors and Drug and Alcohol Dependence,publishing the greatest number of papers on these topics.Most studies focused on psychedelic intervention for PTSD.Three timelines show an“ebb and flow”phenomenon between“substance use/marijuana abuse”and“psychedelic medicine/medicinal cannabis.”Other phytochemicals account for a small proportion of the research and focus on topics like neurosteroid turnover,serotonin levels,and brain-derived neurotrophic factor expression.Conclusion:Research on phytochemicals and PTSD is unevenly distributed across countries/regions,disciplines,and journals.Since 2015,the research paradigm shifted to constitute the mainstream of psychedelic research thus far,leading to the exploration of botanical active ingredients and molecular mechanisms.Other studies focus on anti-oxidative stress and anti-inflammation.

Protective effects of methane-rich saline on mice with allergic asthma by inhibiting inflammatory response, oxidative stress and apoptosis

Background:Asthma is a common cause of breathing difficulty in children and adults,and is characterized by chronic airway inflammation that is poorly controlled by available treatments.This results in severe disability and applies a huge burden to the public health system.Methane has been demonstrated to function as a therapeutic agent in many diseases.The aim of the present study was to explore the effect of methane-rich saline(MRS)on the pathophysiology of a mouse model of asthma and its underlying mechanism.Methods:A murine model of ovalbumin(OVA)-induced allergic asthma was applied in this study.Mice were divided into three groups:a control group,an OVA group,and OVA-induced asthmatic mice treated with MRS as the third group.Lung resistance index(RI)and dynamic compliance(Cdyn)were measured to determine airway hyper-responsiveness(AHR).Haematoxylin and eosin(H&E)staining was performed and scored to show histopathological changes.Cell counts of bronchoalveolar lavage fluid(BALF)were recorded.Cytokines interleukin(IL)-4,IL-5,IL-13,tumor necrosis factorα(TNF-α),and C-X-C motif chemokine ligand 15(CXCL15)from BALF and serum were measured by enzyme-linked immunosorbent assay(ELISA).The oxidative stress indexes,including malondialdehyde(MDA),superoxide dismutase(SOD),catalase(CAT),glutathione(GSH),myeloperoxidase(MPO),and 8-hydroxydeoxyguanosine(8-OHdG),were determined using commercial kits.Apoptosis was evaluated by western blot,quantitative real-time polymerase chain reaction(qRT-PCR),and biochemical examination.Results:MRS administration reversed the OVA-induced AHR,attenuated the pathological inflammatory infiltration,and decreased the cytokines IL-4,IL-5,IL-13,TNF-α,and CXCL15 in serum and BALF.Moreover,following MRS administration,the oxidative stress was alleviated as indicated by decreased MDA,MPO,and 8-OHdG,and elevated SOD and GSH.In addition,MRS exhibited an anti-apoptotic effect in this model,protecting epithelial cells from damage.Conclusions:Methane improves pulmonary function and decreases infiltrative inflammatory cells in the allergic asthmatic mouse model.This may be associated with its anti-inflammatory,antioxidative,and anti-apoptotic properties.