Porcine reproductive and respiratory syndrome(PRRS)is a globally prevalent contagious disease caused by the positive-strand RNA PRRS virus(PRRSV),resulting in substantial economic losses in the swine industry.Modifyin...Porcine reproductive and respiratory syndrome(PRRS)is a globally prevalent contagious disease caused by the positive-strand RNA PRRS virus(PRRSV),resulting in substantial economic losses in the swine industry.Modifying the CD163 SRCR5 domain,either through deletion or substitution,can eff1ectively confer resistance to PRRSV infection in pigs.However,large fragment modifications in pigs inevitably raise concerns about potential adverse effects on growth performance.Reducing the impact of genetic modifications on normal physiological functions is a promising direction for developing PRRSV-resistant pigs.In the current study,we identified a specific functional amino acid in CD163 that influences PRRSV proliferation.Viral infection experiments conducted on Marc145 and PK-15CD163 cells illustrated that the mE535G or corresponding pE529G mutations markedly inhibited highly pathogenic PRRSV(HP-PRRSV)proliferation by preventing viral binding and entry.Furthermore,individual viral challenge tests revealed that pigs with the E529G mutation had viral loads two orders of magnitude lower than wild-type(WT)pigs,confirming effective resistance to HP-PRRSV.Examination of the physiological indicators and scavenger function of CD163 verified no significant differences between the WT and E529G pigs.These findings suggest that E529G pigs can be used for breeding PRRSV-resistant pigs,providing novel insights into controlling future PRRSV outbreaks.展开更多
1. Objective The problems associated with traditional equipment for sampling shallow drilling sediment cores include high production cost, short sample preservation time, inability to store in a freezer, and poor resi...1. Objective The problems associated with traditional equipment for sampling shallow drilling sediment cores include high production cost, short sample preservation time, inability to store in a freezer, and poor resistance to seawater corrosion (Table 1). We developed a sampler with a new design that overcame these problems. The new design worked well in our study and can efficiently support coastal wetland geological surveys.展开更多
1.Objective To measure marine primary productivity as precisely as possible and solve the issues existed in current devices, a new design based on the classical oxygen light-dark bottle methodology (Staehr PA et al., ...1.Objective To measure marine primary productivity as precisely as possible and solve the issues existed in current devices, a new design based on the classical oxygen light-dark bottle methodology (Staehr PA et al., 2010) and integrated with new compact models of ‘simulated’ in situ (SIS) incubations was developed in this study, and it worked well in efficiently supporting our geological survey.展开更多
基金Major Scientific and Technological Projects in Agricultural Biological Breeding of China(2023ZD0404302)Youth Program of National Natural Science Foundation of China(32202754)。
文摘Porcine reproductive and respiratory syndrome(PRRS)is a globally prevalent contagious disease caused by the positive-strand RNA PRRS virus(PRRSV),resulting in substantial economic losses in the swine industry.Modifying the CD163 SRCR5 domain,either through deletion or substitution,can eff1ectively confer resistance to PRRSV infection in pigs.However,large fragment modifications in pigs inevitably raise concerns about potential adverse effects on growth performance.Reducing the impact of genetic modifications on normal physiological functions is a promising direction for developing PRRSV-resistant pigs.In the current study,we identified a specific functional amino acid in CD163 that influences PRRSV proliferation.Viral infection experiments conducted on Marc145 and PK-15CD163 cells illustrated that the mE535G or corresponding pE529G mutations markedly inhibited highly pathogenic PRRSV(HP-PRRSV)proliferation by preventing viral binding and entry.Furthermore,individual viral challenge tests revealed that pigs with the E529G mutation had viral loads two orders of magnitude lower than wild-type(WT)pigs,confirming effective resistance to HP-PRRSV.Examination of the physiological indicators and scavenger function of CD163 verified no significant differences between the WT and E529G pigs.These findings suggest that E529G pigs can be used for breeding PRRSV-resistant pigs,providing novel insights into controlling future PRRSV outbreaks.
基金This equipment has obtained the national invention patent and the patent number is ZL 201520003115.0 (Fig. 3). This study was jointly funded by the Natural Science Foundations of China (41406082,41240022,41706057)National Key R&D Program of China (2016YFE0109600)Governmental Public Research Funds of China (201111023,DD20189503 and GZH201200503).
文摘1. Objective The problems associated with traditional equipment for sampling shallow drilling sediment cores include high production cost, short sample preservation time, inability to store in a freezer, and poor resistance to seawater corrosion (Table 1). We developed a sampler with a new design that overcame these problems. The new design worked well in our study and can efficiently support coastal wetland geological surveys.
基金jointly funded by National Natural Science Foundation of China (Grant No. 41306175)Science and Technology Development Project in Shinan District (2013-15-007-JY)+1 种基金Funding of Scientific and Technological Activities for Scholars from Abroad,and International Science&Technology Cooperation Projects of China (Grant No. 2016YFE0109600)China Geological Survey (Grant Nos. DD20189503 and DD20160144).
文摘1.Objective To measure marine primary productivity as precisely as possible and solve the issues existed in current devices, a new design based on the classical oxygen light-dark bottle methodology (Staehr PA et al., 2010) and integrated with new compact models of ‘simulated’ in situ (SIS) incubations was developed in this study, and it worked well in efficiently supporting our geological survey.
