Alterations of p53 and PCNA in cancer and adjacent tissues from concurrent carcinomas of the esophagus and gastric cardia in the same patient in Linzhou,a high incidence area for esophageal cancer in northern China

AIM: To characterize the alteration and significance of p53and PCNA in cancer and adjacent tissues of concurrent cancersfrom the esophagus and gastric cardia in the same patient.METHODS: P53 and PCNA protein accumulation in 25patients with concurrent cancers from the esophagus andgastric cardia (CC, concurrent carcinomas of esophagealsquamous cell carcinoma and gastric cardia adenocarcinoma)were detected by immunohistochemical method (ABC).RESULTS: In CC patients, both esophageal squamous cellcarcinoma (SCC) and gastric cardia adenocarcinoma (GCA)tissues showed different positive immunostaining extent ofp53 and PCNA protein (P＞0.05). The positive immunostainingrates for p53 and PCNA were 60 % (15/25) and 92 % (23/25), respectively in SCC; and 40 % (10/25) and 88 % (22/25), respectively in GCA. 'Diffuse' immunostaining patternwas frequently observed in both p53 and PCNA. Highcoincidence rates for p53 and PCNA positive staining wereobserved in SCC and GCA from the same patients, andaccounted for 56 % and 96 %. In SCC patients, with thelesions progressed from normal esophageal epithelium (NOR)to basal cell hyperplasia (BCH) to dysplasia (DYS) tocarcinomain situ (CIS) to SCC, the positive rates for p53were 27 %, 50 %, 50 %, 29 % and 72 %, and 55 %, 70 %,75 %, 71% and 93 % for PCNA, respectively. In GCA, withthe lesions progressed from normal gastric cardia epitheliumto DYS to CIS to GCA, the positive rates of p53 expressionwere 44 %, 27 %, 22 % and 36 % respectively, the differencewas not significant; the positive rates of PCNA proteinexpression were 67 %, 64 %, 67 % and 86 %, respectively.The x2 test, Fisher's Exact Test, Mantel-Haenszel x2 Testand Kappa Test were used for the statistics.CONCLUSION: The high coincident alterations for P53 andPCNA in SCC and GCA from the same patient indicate thepossibility of similar molecular basis, which providesimportant molecular basis and etiological clue for similargeographic distribution and risk factors in SCC and GCA.

The Preparation of Perovskite Nano Powder by Sol-gel from DTPA and the Mechanism of Thermal Decomposition

Dy0.8Sr0.2FeO3 nano powder, a synthetic oxide, is made by sol-gel method from metal nitrate and diethylenetriaminepentaacetic acid (DTPA), and the processing parameters are optimized. The process of the preparation, thermal decomposition and the property of the powder are studied by TG-DTA, IR, TEM, and XRD. The diameter of the average grain is about 70 nm. This new technique can be used in the preparation and the studying of na'no materials in the complex oxide system.

Defect Accumulation and Its Effect on Photoluminescence in GaN Bombarded with Low-energy Heavy Ions

Gallium Nitride (GaN) is an important material for the development of novel short-wave-length photonicdevices or high-frequency, high-power electronic devices. Ion implantation/irradiation was proved to be an effective method to modify the physical properties of the material. In the present work, we studied the dependence of damage accumulation on irradiation dose and temperature and the corresponding effects on photolumines cence character of the material. Specimens of GaN (n-type doping, (0001) on axis) were irradiated with