A heat-resistant Pyropia yezoensis strain YZ-4 was isolated from the regenerated blades of single somatic cells of the wild-type(WT)blades treated with 60Co-g-irradiation.When cultured at 23C and 24C,the survival an...A heat-resistant Pyropia yezoensis strain YZ-4 was isolated from the regenerated blades of single somatic cells of the wild-type(WT)blades treated with 60Co-g-irradiation.When cultured at 23C and 24C,the survival and division rates of the YZ-4 conchospores were significantly higher than those of WT(P<0.01).After 16 days in culture the survival rates of YZ-4 conchospores were 71.2%and 60.8%at 23C and 24C,respectively,and the division rates of the surviving conchospores were 100%in both cases.Under the same culture conditions,the survival rates of WT conchospores were only 16.9%and 11.5%,and the division rate 99.8%and 81.8%respectively.The resistance of 50-day-aged F1 gametophytic blades of YZ-4 to the temperature rebound was also significantly higher than those of WT(P<0.01).After 30 days in culture at 18C,23C and 24C,the mean blade length increased by 8.5,4.3 and 2.3 times in YZ-4 and was only 3,0.6 and 0.4 times in WT,respectively.The mean blade weight increased 191.9,85.4 and 66.2 times in YZ-4,but only 78.2,7.8 and 4.6 times in WT.On the 15th day WT blades began to decay,but no decay was observed in YZ-4 blades even after 45 days in culture at 23C and 24C.The results obtained indicate that YZ-4 has a higher resistance to higher temperature insults than WT and it might be of value for commercial production of P.yezoensis.展开更多
Conchospores released from a wild-type strain of Pyropia suborbiculata were cultured in the laboratory for 20-80 days.Mother blades of different ages were enzymatically isolated to obtain single cells,which then devel...Conchospores released from a wild-type strain of Pyropia suborbiculata were cultured in the laboratory for 20-80 days.Mother blades of different ages were enzymatically isolated to obtain single cells,which then developed into different types of regenerative plants within a liquid medium.These regenerative plant types includes:normal blades,abnormal blades,cell-masses,and sexual cell-masses.The age of mother blades distinctly affected the number and proportion of each type of regenerative plants.When the age of mother blades increased from 20 to 80 days,the proportion of normal blades in the isolated cells sharply decreased,the percentage of abnormal blades increased,and the proportion of cell-masses was relatively unchanged.After 40 days of cultivation,sexual cell-masses appeared in regenerated plants derived from the single blade cells of the PS-WT strain,and gradually increased with mother cell age.In addition,the proportion of normal blades decreased in regenerated plants sourced from the basal to apical parts of the same alga,while the proportion of abnormal blades and cell-masses increased.In summary,the isolated cells of the gametophytic blade of P.suborbiculata developed into different types of regenerated plantlets in vivo due to the different stages of cell differentiation.We preliminarily concluded that the differentiation from conchospores to the sexual cells could be divided into at least seven different groups.展开更多
基金the National High Technology Research&Development Program of China(“863”Program)(Grant No.2006AA10A413)the National Natural Science Foundation of China(Grant No.31072208)+2 种基金the Shanghai Natural Science Foundation(Grant No.05RZ14110)Science and Technology Commission of Shanghai Municipality(Grant No.07XD14028)Shanghai Municipal Education Commission(Project No.J50701).
文摘A heat-resistant Pyropia yezoensis strain YZ-4 was isolated from the regenerated blades of single somatic cells of the wild-type(WT)blades treated with 60Co-g-irradiation.When cultured at 23C and 24C,the survival and division rates of the YZ-4 conchospores were significantly higher than those of WT(P<0.01).After 16 days in culture the survival rates of YZ-4 conchospores were 71.2%and 60.8%at 23C and 24C,respectively,and the division rates of the surviving conchospores were 100%in both cases.Under the same culture conditions,the survival rates of WT conchospores were only 16.9%and 11.5%,and the division rate 99.8%and 81.8%respectively.The resistance of 50-day-aged F1 gametophytic blades of YZ-4 to the temperature rebound was also significantly higher than those of WT(P<0.01).After 30 days in culture at 18C,23C and 24C,the mean blade length increased by 8.5,4.3 and 2.3 times in YZ-4 and was only 3,0.6 and 0.4 times in WT,respectively.The mean blade weight increased 191.9,85.4 and 66.2 times in YZ-4,but only 78.2,7.8 and 4.6 times in WT.On the 15th day WT blades began to decay,but no decay was observed in YZ-4 blades even after 45 days in culture at 23C and 24C.The results obtained indicate that YZ-4 has a higher resistance to higher temperature insults than WT and it might be of value for commercial production of P.yezoensis.
基金The study was supported in part by the National Key Research and Development Program of China(2018YFD0900606)National Natural Science Foundation of China(31072208)+2 种基金Major Science and Technology Specific Program of Zhejiang Province(2016C02055-6)Science and Technology Planning Project of Jiangsu Province,China(BE2018335)Open Program of Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province(2017fjscq02).
文摘Conchospores released from a wild-type strain of Pyropia suborbiculata were cultured in the laboratory for 20-80 days.Mother blades of different ages were enzymatically isolated to obtain single cells,which then developed into different types of regenerative plants within a liquid medium.These regenerative plant types includes:normal blades,abnormal blades,cell-masses,and sexual cell-masses.The age of mother blades distinctly affected the number and proportion of each type of regenerative plants.When the age of mother blades increased from 20 to 80 days,the proportion of normal blades in the isolated cells sharply decreased,the percentage of abnormal blades increased,and the proportion of cell-masses was relatively unchanged.After 40 days of cultivation,sexual cell-masses appeared in regenerated plants derived from the single blade cells of the PS-WT strain,and gradually increased with mother cell age.In addition,the proportion of normal blades decreased in regenerated plants sourced from the basal to apical parts of the same alga,while the proportion of abnormal blades and cell-masses increased.In summary,the isolated cells of the gametophytic blade of P.suborbiculata developed into different types of regenerated plantlets in vivo due to the different stages of cell differentiation.We preliminarily concluded that the differentiation from conchospores to the sexual cells could be divided into at least seven different groups.
