Construction of Electrochemical Biosensors Based on the CRISPR/Cas12 System for Applications

The current major issue in improving detection sensitivity and selectivity is to design an electrochemical sensor that does not require PCR amplification for nucleic acid identification and measurement. Because of their great sensitivity, precision, and simplicity of downsizing, electrochemical biosensors have emerged as a research hotspot in the field of nucleic acid detection. The CRISPR/Cas12 system has emerged as a potent tool for nucleic acid detection due to its powerful cleavage activity and selectivity. Specific electrode changes combined with the CRISPR/Cas12 system can greatly improve the performance of electrochemical biosensors. In this study, the design concepts of electrochemical biosensors based on the CRISPR/Cas12 system and their application advancements in nucleic acid detection are discussed.

Remaining Useful Life Model and Assessment of Mechanical Products: A Brief Review and a Note on the State Space Model Method

The remaining useful life(RUL) prediction of mechanical products has been widely studied for online system performance reliability, device remanufacturing, and product safety(safety awareness and safety improvement). These studies incorporated many di erent models, algorithms, and techniques for modeling and assessment. In this paper, methods of RUL assessment are summarized and expounded upon using two major methods: physics model based and data driven based methods. The advantages and disadvantages of each of these methods are deliberated and compared as well. Due to the intricacy of failure mechanism in system, and di culty in physics degradation observation, RUL assessment based on observations of performance variables turns into a science in evaluating the degradation. A modeling method from control systems, the state space model(SSM), as a first order hidden Markov, is presented. In the context of non-linear and non-Gaussian systems, the SSM methodology is capable of performing remaining life assessment by using Bayesian estimation(sequential Monte Carlo). Being e ective for non-linear and non-Gaussian dynamics, the methodology can perform the assessment recursively online for applications in CBM(condition based maintenance), PHM(prognostics and health management), remanufacturing, and system performance reliability. Finally, the discussion raises concerns regarding online sensing data for SSM modeling and assessment of RUL.

Flexible Conductive Anodes Based on 3D Hierarchical Sn/NS-CNFs@rGO Network for Sodium-Ion Batteries

Metallic Sn has provoked tremendous progress as an anode material for sodium-ion batteries(SIBs).However,Sn anodes suffer from a dramatic capacity fading,owing to pulverization induced by drastic volume expansion during cycling.Herein,a flexible three-dimensional(3D)hierarchical conductive network electrode is designed by constructing Sn quantum dots(QDs)encapsulated in one-dimensional N,S codoped carbon nanofibers(NS-CNFs)sheathed within two-dimensional(2D)reduced graphene oxide(rGO)scrolls.In this ingenious strategy,1D NS-CNFs are regarded as building blocks to prevent the aggregation and pulverization of Sn QDs during sodiation/desodiation,2D rGO acts as electrical roads and“bridges”among NS-CNFs to improve the conductivity of the electrode and enlarge the contact area with electrolyte.Because of the unique structural merits,the flexible 3D hierarchical conductive network was directly used as binder-and current collectorfree anode for SIBs,exhibiting ultra-long cycling life(373 mAh g?1 after 5000 cycles at 1 A g?1),and excellent high-rate capability(189 mAh g?1 at 10 A g?1).This work provides a facile and efficient engineering method to construct 3D hierarchical conductive electrodes for other flexible energy storage devices.

Integration of high-performance spin-orbit torque MRAM devices by 200-mm-wafer manufacturing platform

We demonstrate in-plane field-free-switching spin-orbit torque(SOT)magnetic tunnel junction(MTJ)devices that are capable of low switching current density,fast speed,high reliability,and,most importantly,manufactured uniformly by the 200-mm-wafer platform.The performance of the devices is systematically studied,including their magnetic properties,switch-ing behaviors,endurance and data retention.The successful integration of SOT devices within the 200-mm-wafer manufactur-ing platform provides a feasible way to industrialize SOT MRAMs.It is expected to obtain excellent performance of the devices by further optimizing the MTJ film stacks and the corresponding fabrication processes in the future.

Temporal and spatial evolution of air-spark switch plasma investigated by the Mach–Zehnder interferometer

An air-spark switch plasma was diagnosed by the Mach–Zehnder laser interferometer with ultra-high spatial and temporal resolution. The interferograms containing plasma phase shift information at different time were obtained. The phase shift distributions of the plasma were extracted by numerically processing the interferograms. The three-dimensional(3 D) electron density distributions of the air-spark switch plasma were then obtained. The working process of the air-spark switch was described by analyzing the temporal and spatial evolution of the plasma electron density.

Demonstration of a manufacturable SOT-MRAM multiplexer array towards industrial applications

We have successfully demonstrated a 1 Kb spin-orbit torque(SOT)magnetic random-access memory(MRAM)multiplexer(MUX)array with remarkable performance.The 1 Kb MUX array exhibits an in-die function yield of over 99.6%.Additionally,it provides a sufficient readout window,with a TMR/RP_sigma%value of 21.4.Moreover,the SOT magnetic tunnel junctions(MTJs)in the array show write error rates as low as 10^(-6)without any ballooning effects or back-hopping behaviors,ensuring the write stability and reliability.This array achieves write operations in 20 ns and 1.2 V for an industrial-level temperature range from-40 to 125℃.Overall,the demonstrated array shows competitive specifications compared to the state-of-the-art works.Our work paves the way for the industrial-scale production of SOT-MRAM,moving this technology beyond R&D and towards widespread adoption.

High repetition granular Co/Pt multilayers with improved perpendicular remanent magnetization for high-density magnetic recording

Thanks to the strong perpendicular magnetic anisotropy(PMA), excellent processing compatibility as well as novel spintronic phenomenon, Co/Pt multilayers have been attracting massive attention and widely used in magnetic storage.However, reversed magnetic domains come into being with the increasing layer repetition ‘N’ to reduce magneto-static energy, resulting in the remarkable diminishment of the remanent magnetization(Mr). As a result, the product of Mrand thickness(i.e., the remanent moment-thickness product, Mrt), a key parameter in magnetic recording for reliable data storing and reading, also decreases dramatically. To overcome this issue, we deposit an ultra-thick granular [Co/Pt]80multilayer with a total thickness of 68 nm on granular SiNxbuffer layer. The Mrt value, Mrto saturation magnetization(Ms) ratio as well as out of plane(OOP) coercivity(Hcoop) are high up to 2.97 memu/cm^(2), 67%, and 1940 Oe(1 Oe = 79.5775 A·m^(-1)),respectively, which is remarkably improved compared with that of continuous [Co/Pt]80multilayers. That is because large amounts of grain boundaries in the granular multilayers can efficiently impede the propagation and expansion of reversed magnetic domains, which is verified by experimental investigations and micromagnetic simulation results. The simulation results also indicate that the value of Mrt, Mr/Msratio, and Hcoopcan be further improved through optimizing the granule size, which can be experimentally realized by manipulating the process parameter of SiNxbuffer layer. This work provides an alternative solution for achieving high Mrt value in ultra-thick Co/Pt multilayers, which is of unneglectable potential in applications of high-density magnetic recording.

On the improved dynamics approach in loop quantum black holes

In this paper,we consider the B?hmer–Vandersloot(BV)model of loop quantum black holes obtained from the improved dynamics approach.We adopt the Saini–Singh gauge,in which it was found analytically that the BV spacetime is geodesically complete.We show that black/white hole horizons do not exist in this geodesically complete spacetime.Instead,there exists only an infinite number of transition surfaces,which always separate trapped regions from anti-trapped ones.Comments on the improved dynamics approach adopted in other models of loop quantum black holes are also given.

超短脉冲激光与液滴相互作用过程物理机理研究进展

超短脉冲激光与液滴相互作用在激光大气传输、光谱增强、极紫外光源以及生物医学等中扮演着重要的角色.这些应用背后涉及多个物理过程,包括等离子体的产生与演化、等离子体的能量沉积、相变和流体动力学等.经过几十年的发展,这一现象背后的物理机理研究已取得巨大进步,助力了相关实际应用的进步,尤其是激光大气传输中的雾霾清除和纳米光刻中极紫外光源的产生.本文综述了超短激光与液滴相互作用物理机理的研究进展,围绕主要物理过程展示最新的研究成果,重点阐释了液滴内部等离子体演化、液滴击穿光谱、超短脉冲激光在大气中的传输、等离子体能量沉积、相变以及液滴形变等现象背后的物理机理,并对当前研究中存在的不足进行了总结,对未来发展进行了展望.

Nitrogen reduction reaction on small iron clusters supported by N-doped graphene:A theoretical study of the atomically precise active-site mechanism

Nonprecious metal catalysts are known of significance for electrochemical N2 reduction reaction(NRR)of which the mechanism has been illustrated by ongoing investigations of single atom catalysis.However,it remains challenging to fully understand the size-dependent synergistic effect of active sites inherited in substantial nanocatalysts.In this work,four types of small iron clusters Fen(n=1–4)supported on nitrogen-doped graphene sheets are constructed to figure out the size dependence and synergistic effect of active sites for NRR catalytic activities.It is revealed that Fe3 and Fe4 clusters on N4G supports exhibit higher NRR activity than single-iron atom and iron dimer clusters,showing lowered limiting potential and restricted hydrogen evolution reaction(HER)which is a competitive reaction channel.In particular,the Fe4-N4G displays outstanding NRR performance for“side-on”adsorption of N2 with a small limiting potential(−0.45 V).Besides the specific structure and strong interface interaction within the Fe4-N4G itself,the high NRR activity is associated with the unique bonding/antibonding orbital interactions of N-N and N-Fe for the adsorptive N2 and NNH intermediates,as well as relatively large charge transfer between N2 and the cluster Fe4-N4G.

High-performance enhancement-mode thin-film transistors based on Mg-doped In2O3 nanofiber networks

Although In2O3 nanofibers （NFs） are well-known candidates as active materials for next-generation, low-cost electronics, these NF based devices still suffer from high leakage current, insufficient on-off current ratios （Ion/Ioff）, and large, negative threshold voltages （VTH）, leading to poor device performance, parasitic energy consumption, and rather complicated circuit design. Here, instead of the conventional surface modification of In2O3 NFs, we present a one-step electrospinning process （i.e., without hot-press） to obtain controllable Mg-doped In2O3 NF networks to achieve high-performance enhancement-mode thin-film transistors （TFTs）. By simply adjusting the Mg doping concentration, the device performance can be manipulated precisely. For the optimal doping concentration of 2 mol%, the devices exhibit a small VTH （3.2 V）, high saturation current （1.1 × 10^-4 A）, large on/off current ratio （〉 10^8）, and respectable peak carrier mobility （2.04 cm2/（V.s））, corresponding to one of the best device performances among all 1D metal-oxide NFs based devices reported so far. When high-K HfOx thin films are employed as the gate dielectric, their electron mobility and VTH can be further improved to 5.30 cm^2/（V.s） and 0.9 V, respectivel）, which demonstrates the promising prospect of these Mg-doped In2O3 NF networks for high- performance, large-scale, and low-power electronics.

Effect of 1070 nm laser intensity on parameters of In_(0.3)Ga_(0.7)As solar cell

The photoelectric properties of In_(0.3)Ga_(0.7) As solar cells applied in laser wireless power transmission(LWPT) were studied when they were irradiated by 1070 nm continuous wave(CW) laser of various intensities. The influences of laser intensity on solar cell parameters extracted by the pollination algorithm were analyzed quantitatively.Results show that the conversion efficiency of the cell rose to the maximum and then decreased rapidly in the laser intensity range of 50–900 mW/cm2. With higher energy laser irradiation, the rise of ideality factor and reverse saturation current would lead to the degradation of voltage at the maximum power point, which was the main reason for the decrease of conversion efficiency. The results provide the basis for choosing the appropriate input energy in the case of different transmission systems.

Energy-aware fuzzy job-shop scheduling for engine remanufacturing at the multi-machine level

The rise of the engine remanufacturing industry has resulted in increased possibilities of energy conservation during the remanufacturing process,and scheduling could exert significant effects on the energy performance of manufacturing systems.However,only a few studies have specifically addressed energy-efficient scheduling for remanufacturing.Considering the uncertain processing time and routes and the operation characteristics of remanufacturing,we used the crankshaft as an illustrative case and built a fuzzy job-shop scheduling model to minimize the energy consumption during remanufacturing.An improved adaptive genetic algorithm was developed by using the hormone modulation mechanism to deal with the scheduling problem that simultaneously involves parallel machines,batch machines,and uncertain processing routes and time.The algorithm demonstrated superior performance in terms of optimal value,run time,and convergent generation in comparison with other algorithms.Computational results indicated that the optimal scheduling scheme is expected to generate 1.7 kW∙h of energy saving for the investigated problem size.In addition,the scheme could improve the energy efficiency of the crankshaft remanufacturing process by approximately 5%.This study provides a basis for production managers to improve the sustainability of remanufacturing through energy-aware scheduling.

Mechanism of field-like torque in spin-orbit torque switching of perpendicular magnetic tunnel junction

The current-induced spin-orbit torque(SOT) is one of the most promising ways for high speed and low power spintronics devices. However, the mechanism of SOT driven magnetization reversal, especially the role of the field-like torque(FLT), is still unclear. Here, we report the observed promotion and suppression of switching by FLT, which depends on the relative direction of FLT and spin polarization. Our results reveal that the FLT could modulate the switching speed and power consumption by affecting the work done by the damping-like torque, and leads two different reversal dynamical paths during the switching.Furthermore, the origin of incubation time in SOT induced switching is clarified simultaneously.

“中华民族共同体意识”研究述评

当前学术界对中华民族共同体意识的研究,呈现出不断深化和具体的形态,成果显著。本文从历史视域对中华民族共同体意识的相关研究进行了回顾和评述:第一,词源概念的认知逐步深入,是马克思主义思想指引下,集中国历史传统与当代现实的结果,既涉及了整体概念的宏观阐述,也关注到具体概念的形成分析。第二,思想和观念的研究是在中国统一多民族国家历史发展的框架下进行的多元表达,不仅注重某一思想和观念的深入探讨,也关注思想的多元阐释以及观念延续性的整体把握和综合论证。第三,交往、交流、交融史视角立足于中华民族多元一体的历史事实,涵括了历史研究中的诸多专题内容和具体方面。第四,其他视角的研究则着重凸显中国共产党在铸牢中华民族共同体意识中的精神引领与作用。

Investigation of the laser ultrasound propagation in K9 glass using optical interferometry

The propagating of laser-generated ultrasonic waves in K9 glass was investigated. Many methods have been developed to detect the laser ultrasound since laser ultrasonic waves can be used to measure material parameters or characterize materials properties. In order to reduce the measuring time, a Mach–Zehnder interferometer, a full field measuring tool,was preferred in this paper. The ultrasonic wave was produced on the K9 glass surface by a Q-switched Nd:YAG laser absorbed in a liquid layer. The interferograms were then taken at various delay times by a CCD camera after single pulse induced laser ultrasonic waves. Ultrasonic waves in the K9 glass can be observed from interferogram images. The results provide an understanding of laser ultrasound propagation in K9 glass in the lifetime.

Photoluminescence properties of Ca_(4)La_(6)（SiO_(4)）_(4)（PO_(4)）_(2)O_(2)-based phosphors for wLEDs

The apatite compound Ca4La6（SiO4）4（PO4）2O2（CLSPO） was explored as the host material for phosphors used in white light-emitting diodes（w LEDs）. The crystal structure of the CLSPO host prepared by the solid-state reaction method was investigated with Rietveld refinement. The rare earth ions(Eu3+/Tb3+/Ce3+, Tb3+/Tb3+, Mn2+)activated CLSPO phosphors were synthesized, and their photoluminescence properties, quantum yields, as well as thermal stabilities, were studied. Under near-ultraviolet excitations, the Eu3+ and Tb3+ -doped CLSPO compounds exhibited red and green emissions with high luminescence efficiencies. Besides, tunable emissions from green to orange were obtained by introducing Mn2+ ions into the Tb3+ -doped CLSPO samples. The results showed that the phosphors studied may have potential applications for w LEDs.