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A nanosecond level current pulse capture taper optical fiber probe based on micron level nitrogen-vacancy color center diamond
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作者 Yuchen Bian Yangfan Mao +5 位作者 honghao chen Shiyu Ge Wentao Lu chengkun Wang Sihan An Guanxiang Du 《Chinese Physics B》 SCIE EI CAS 2024年第12期39-44,共6页
This work demonstrates a micron-sized nanosecond current pulse probe using a quantum diamond magnetometer.A micron-sized diamond crystal affixed to a fiber tip is integrated on the end of a conical waveguide.We demons... This work demonstrates a micron-sized nanosecond current pulse probe using a quantum diamond magnetometer.A micron-sized diamond crystal affixed to a fiber tip is integrated on the end of a conical waveguide.We demonstrate real-time visualization of a single 100 nanosecond pulse and discrimination of two pulse trains of different frequencies with a coplanar waveguide and a home-made PCB circuit.This technique finds promising applications in the display of electronic stream and can be used as a pulse discriminator to simultaneously receive and demodulate multiple pulse frequencies.This method of detecting pulse current is expected to provide further detailed analysis of the internal working state of the chip. 展开更多
关键词 pulse capture nitrogen-vacancy(NV)color center current imaging chip detection
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Cloning and characterization of the gene encoding β-amyrin synthase in the glycyrrhizic acid biosynthetic pathway in Glycyrrhiza uralensis 被引量:6
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作者 honghao chen Ying Liu +2 位作者 Xiaoqin Zhang Xinjie Zhan Chunsheng Liu 《Acta Pharmaceutica Sinica B》 SCIE CAS 2013年第6期416-424,共9页
Glycyrrhiza uralensis is considered to be one of the most important herbs in traditional Chinese medicine due to its numerous pharmacological effects particularly its ability to relieve cough and act as a mucolytic.Ba... Glycyrrhiza uralensis is considered to be one of the most important herbs in traditional Chinese medicine due to its numerous pharmacological effects particularly its ability to relieve cough and act as a mucolytic.Based on previous research,these effects are mediated by a number of active ingredients,especially glycyrrhizic acid(GA).In the present study,a gene encodingβ-amyrin synthase(β-AS)involved in GA biosynthesis in G.uralensis has been cloned and expressed in Saccharomyces cerevisiae.The cloned enzyme showed similar activity to native enzymes isolated from other Glycyrrhiza species to catalyze the conversion of 2,3-oxidosqualene intoβ-amyrin.In fact theβ-AS gene is particularly important in the GA biosynthetic pathway in G.uralensis.The complete sequence of the enzyme was determined and a phylogenetic tree based on theβ-AS gene of G.uralensis and 20 other species was created.This showed that Glycyrrhiza glabra had the closest kinship with G.uralensis.The results of this work will be useful in determining how to improve the efficacy of G.uralensis by improving its GA content and in exploring the biosynthesis of GA in vitro. 展开更多
关键词 β-Amyrin synthase Glycyrrhiza uralensis Glycyrrhizic acid Molecular cloning Heterologous expression GC–MS
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