The 16S rDNA amplicon high-throughput sequencing technique provides a robust and inexpensive approach to detect the gut microbiota of amphibians. Since different experimental protocols generate technical biases in dra...The 16S rDNA amplicon high-throughput sequencing technique provides a robust and inexpensive approach to detect the gut microbiota of amphibians. Since different experimental protocols generate technical biases in drawing the gut microbiota profiles, the integrative analysis of gut microbiota produced by different studies must be performed with circumspection. In this study, we compared the efficacy of two DNA extraction methods （i.e., a phenol-chloroform method and TIANamp Stool DNA Kit） in describing intestinal and fecal bacterial communities of transplanted Asiatic toad （Bufo gargarizans） tadpoles. In terms of the DNA extraction quality （i.e., DNA purity and yield rate） and the consistency in between fecal and intestinal microbiota structures （i.e., a and 13 diversity indices）, the phenol-chloroform method was more robust than this commercial stool kit in profiling gut microbiota of tadpoles with feces.展开更多
基金supported by the National Natural Science Foundation of China (NSFC 31600104)Key Scientific Research Project of Higher Education in Henan Province (No. 17B180004)+2 种基金National Undergraduate Training Program for Innovation and Entrepreneurship (No. 201610477013)Ph.D. Research Startup Foundation of Xinyang Normal University (No. 0201424)Nanhu Scholars Program for Young Scholars of Xinyang Normal University
文摘The 16S rDNA amplicon high-throughput sequencing technique provides a robust and inexpensive approach to detect the gut microbiota of amphibians. Since different experimental protocols generate technical biases in drawing the gut microbiota profiles, the integrative analysis of gut microbiota produced by different studies must be performed with circumspection. In this study, we compared the efficacy of two DNA extraction methods （i.e., a phenol-chloroform method and TIANamp Stool DNA Kit） in describing intestinal and fecal bacterial communities of transplanted Asiatic toad （Bufo gargarizans） tadpoles. In terms of the DNA extraction quality （i.e., DNA purity and yield rate） and the consistency in between fecal and intestinal microbiota structures （i.e., a and 13 diversity indices）, the phenol-chloroform method was more robust than this commercial stool kit in profiling gut microbiota of tadpoles with feces.
