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Hc-transgelin is a novel matrix protein gene involved in the shell biomineralization of triangle sail mussel(Hyriopsis cumingii)
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作者 Yongbin Yuan honghui hu +4 位作者 Jinlu Li Ling Yan Jingyan Zhong Zhiyi Bai Jiale Li 《Aquaculture and Fisheries》 2024年第4期543-550,共8页
In triangle sail mussel(Hyriopsis cumingii),shell biomineralization is a complicated process that involves multiple gene products.Shell matrix proteins are involved in the formation of the organic framework and play a... In triangle sail mussel(Hyriopsis cumingii),shell biomineralization is a complicated process that involves multiple gene products.Shell matrix proteins are involved in the formation of the organic framework and play an important role in the regulation of calcium carbonate deposition.In this study,A new shell matrix protein gene Hc-transgelin was identified in H.cumingii.The full-length cDNA of Hc-transgelin was 1200 bp,including a 501 bp open reading frame,which encoded 166 amino acids.Hc-transgelin is rich in lysine,it accounts for 11.40%of the protein.The predicted transgelin protein contained a conserved calmodulin homologous domain.A tissue-specific expression assay indicated that Hc-transgelin exhibited significantly highest expression in the mantle.Furthermore,Hc-transgelin in situ hybridization detected positive signals at the edge of the mantle outer fold,where nacre and prismatic layer biomineralization occur.An RNA interference assay showed that the shape of aragonite flakes in nacre changed and their growth was inhibited,and cracks appeared in the prismatic layer organic sheath when the expression of Hc-transgelin was suppressed.In a shell repair assay,a higher expression of Hc-transgelin appeared from day 12 to day 25 when the nacre accumulated quickly.These findings indicate that Hc-transgelin may be involved in the formation of aragonite flakes in the nacre and play a role in the formation of the organic sheath in the prismatic layer of the shell.This study provides new insights into the role of the Hc-transgelin gene and also contributes to the molecular understanding of mollusk shell formation. 展开更多
关键词 Hyriopsis cumingii Biomineralization Matrix protein Hc-transgelin
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