[Objective] This study aimed to investigate the genetic relationship among waxberry （Myrica rubra Bieb. et Zucc） cultivars in Jiangsu Province. [ Method] Eighteen waxberry cultivars （lines） in Jiangsu Province wer...[Objective] This study aimed to investigate the genetic relationship among waxberry （Myrica rubra Bieb. et Zucc） cultivars in Jiangsu Province. [ Method] Eighteen waxberry cultivars （lines） in Jiangsu Province were analyzed using ISSR-PCR method, and molecular phylogenetic tree was constructed using UPGNA method. [ Result ~ A total of 70 DNA fragments were amplified with nine screened primers, including 43 polymorphic fragments, accounting for 61.4% of the total number of amplified fragments; 18 waxberry cultivars （lines） were clustered into 6 categories with the genetic distance of 0. 14, indicating that there is partial isolation on the basis of gene exchange between waxberry cultivars （lines） in Jiangsu Province. [ Conclusion] This study provided scientific basis for the protection and utilization of waxberry germplasm resources and cultivation of new varieties.展开更多
基金Supported by Special Fund for National Public Service Sectors (Agriculture) Research of China(20120389)Scientific and Technological Support Project of Suzhou City (SN201008)+3 种基金Public Technology Service Platform for Characteristic Fruit Industry of Suzhou City(SZP201011)Natural Science Research Project from Suzhou Polytechnic Institute of Agriculture(NS1101)Jiangsu Agricultural Science and Technology Innovation Fund[CX(12)2011]Jiangsu Provincial Science&Technology Support (Agriculture) Program(BE2012361)
文摘[Objective] This study aimed to investigate the genetic relationship among waxberry （Myrica rubra Bieb. et Zucc） cultivars in Jiangsu Province. [ Method] Eighteen waxberry cultivars （lines） in Jiangsu Province were analyzed using ISSR-PCR method, and molecular phylogenetic tree was constructed using UPGNA method. [ Result ~ A total of 70 DNA fragments were amplified with nine screened primers, including 43 polymorphic fragments, accounting for 61.4% of the total number of amplified fragments; 18 waxberry cultivars （lines） were clustered into 6 categories with the genetic distance of 0. 14, indicating that there is partial isolation on the basis of gene exchange between waxberry cultivars （lines） in Jiangsu Province. [ Conclusion] This study provided scientific basis for the protection and utilization of waxberry germplasm resources and cultivation of new varieties.
