[ Objective] This study analyzed the differences in DDX3 Y gene sequences among male horse, male donkey, male mule, male hinny and male foal to investigate the relationship between DDX3Y gene structure and the mechani...[ Objective] This study analyzed the differences in DDX3 Y gene sequences among male horse, male donkey, male mule, male hinny and male foal to investigate the relationship between DDX3Y gene structure and the mechanism of infertility in male Equus fetus × asirms. [ Method] Partial sequences of DDX3Y gene in male horse, male donkey, male mule, male hinny and male foal were obtained by genome comparison, sequencing and cloning, and compared by bioinformatics methods. [ Result] The CDS regions varied in 5 base pairs and two amino acids. DDX3Y protein was predicted to be an unstable protein. In addition, the results also showed that DDX3 Y gene in male horse displayed a closer phylogenetic relationship to male mule and male foal; DDX3Y gene in male donkey displayed a closer phylogenetic relationship to male hinny. [ Conclusion] This study provided scientific basis for further exploring the function of DDX3 Y gene and the mecha- nism of reproductive regulation in male Equus ferus × asinus.展开更多
[Object]This study aimed to examine the effects of 3,7-dimethyl-1-（5-oxo-hexyl）-xanthine（pentoxifylline,PF）on the motility,mitochondrial activity,acrosome integrity and fertilization rate of spermatozoa in both fr...[Object]This study aimed to examine the effects of 3,7-dimethyl-1-（5-oxo-hexyl）-xanthine（pentoxifylline,PF）on the motility,mitochondrial activity,acrosome integrity and fertilization rate of spermatozoa in both fresh and frozen-thawed bull semen.[Method]Fresh and frozen-thawed bull spermatozoa were exposed to 5 mmol/L PF with untreated samples as controls.[Result]Fresh spermatozoa showed reduced（P〈0.05）motility after 2 h incubation with PF whereas,surprisingly,frozen-thawed samples exhibited increased sperm motility（P〈0.05）after 2 h incubation with PF and they also showed enhanced longevity compared to controls.Mitochondrial activity in both fresh and frozen-thawed bull spermatozoa increased（P〈0.05）during 4 h incubation with PF whereas acrosome integrity remained unchanged in both types of semen.However,treatment with 5 mmol/L PF did not influence the in vitro fertilization efficiency of fresh spermatozoa but improved significantly（P〈0.05）that of frozen-thawed spermatozoa.[Conclusion]These results indicate that PF can improve sperm quality of frozenthawed bull semen,and may improve pregnancy rates in bovine artificial insemination programmes employing frozen semen.展开更多
基金Supported by Project of Science and Technology Department of Inner Mongolia Autonomous Region
文摘[ Objective] This study analyzed the differences in DDX3 Y gene sequences among male horse, male donkey, male mule, male hinny and male foal to investigate the relationship between DDX3Y gene structure and the mechanism of infertility in male Equus fetus × asirms. [ Method] Partial sequences of DDX3Y gene in male horse, male donkey, male mule, male hinny and male foal were obtained by genome comparison, sequencing and cloning, and compared by bioinformatics methods. [ Result] The CDS regions varied in 5 base pairs and two amino acids. DDX3Y protein was predicted to be an unstable protein. In addition, the results also showed that DDX3 Y gene in male horse displayed a closer phylogenetic relationship to male mule and male foal; DDX3Y gene in male donkey displayed a closer phylogenetic relationship to male hinny. [ Conclusion] This study provided scientific basis for further exploring the function of DDX3 Y gene and the mecha- nism of reproductive regulation in male Equus ferus × asinus.
基金Supported by NMKJ Project of Production and Industrial Application of Sexed Semen in Domestic Animals(No.20111701)
文摘[Object]This study aimed to examine the effects of 3,7-dimethyl-1-（5-oxo-hexyl）-xanthine（pentoxifylline,PF）on the motility,mitochondrial activity,acrosome integrity and fertilization rate of spermatozoa in both fresh and frozen-thawed bull semen.[Method]Fresh and frozen-thawed bull spermatozoa were exposed to 5 mmol/L PF with untreated samples as controls.[Result]Fresh spermatozoa showed reduced（P〈0.05）motility after 2 h incubation with PF whereas,surprisingly,frozen-thawed samples exhibited increased sperm motility（P〈0.05）after 2 h incubation with PF and they also showed enhanced longevity compared to controls.Mitochondrial activity in both fresh and frozen-thawed bull spermatozoa increased（P〈0.05）during 4 h incubation with PF whereas acrosome integrity remained unchanged in both types of semen.However,treatment with 5 mmol/L PF did not influence the in vitro fertilization efficiency of fresh spermatozoa but improved significantly（P〈0.05）that of frozen-thawed spermatozoa.[Conclusion]These results indicate that PF can improve sperm quality of frozenthawed bull semen,and may improve pregnancy rates in bovine artificial insemination programmes employing frozen semen.
