目的探讨与肝细胞癌(HCC)远处转移相关的DNA拷贝数变异(copy number alteration,CNA)分子标志。方法采用高分辨率Agilent 244K微阵列比较基因组杂交(aCGH)方法检测63例HCC样本基因组DNA的CNA特征。以Log-rank检验、Kaplan-Meier生存分...目的探讨与肝细胞癌(HCC)远处转移相关的DNA拷贝数变异(copy number alteration,CNA)分子标志。方法采用高分辨率Agilent 244K微阵列比较基因组杂交(aCGH)方法检测63例HCC样本基因组DNA的CNA特征。以Log-rank检验、Kaplan-Meier生存分析以及Cox风险比例模型,分析各DNA片段CNA与HCC远处转移的相关性。结果染色体片段12p12.2-13.31丢失是HCC患者远处转移的显著危险因素(P<0.01,Log-rank检验)。与非丢失者相比,12p12.2-13.31丢失HCC患者的远处转移风险比(hazard ratio,HR)为22.98(95%CI=4.29~123.22,P<0.01)。多变量Cox回归分析显示,12p12.2-13.31丢失是HCC远处转移的独立预测因素(HR=7.94,95%CI=1.14~55.61,P<0.05)。结论染色体片段12p12.2-13.31丢失增加HCC远处转移风险,可作为预测HCC远处转移的一个分子标志。展开更多
Trace metal elements, such as iron, copper, manganese, and zinc, are essential nutrients for biological processes. Although their intake demand is low, they play a crucial role in cell homeostasis as the cofactors of ...Trace metal elements, such as iron, copper, manganese, and zinc, are essential nutrients for biological processes. Although their intake demand is low, they play a crucial role in cell homeostasis as the cofactors of various enzymes. Symbiotic intestinal microorganisms compete with their host for the use of trace metal elements. Moreover, the metabolic processes of trace metal elements in the host and microorganisms affect the organism's health. Supplementation or the lack of trace metal elements in the host can change the intestinal microbial community structure and function. Functional changes in symbiotic microorganisms can affect the host's metabolism of trace metal elements. In this review, we discuss the absorption and transport processes of trace metal elements in the host and symbiotic microorganisms and the effects of dynamic changes in the levels of trace metal elements on the intestinal microbial community structure. We also highlight the participation of trace metal elements as enzyme cofactors in the host immune process. Our findings indicate that the host uses metal nutrition immunity or metal poisoning to resist pathogens and improve immunity.展开更多
Small GTPases including Ras,Rho,Rab,Arf,and Ran are omnipresent molecular switches in regulating key cellular functions.Their dysregulation is a therapeutic target for tumors,neurodegeneration,cardiomyopathies,and inf...Small GTPases including Ras,Rho,Rab,Arf,and Ran are omnipresent molecular switches in regulating key cellular functions.Their dysregulation is a therapeutic target for tumors,neurodegeneration,cardiomyopathies,and infection.However,small GTPases have been historically recognized as“undruggable”.Targeting KRAS,one of the most frequently mutated oncogenes,has only come into reality in the last decade due to the development of breakthrough strategies such as fragment-based screening,covalent ligands,macromolecule inhibitors,and PROTACs.Two KRAS^(G12C)covalent inhibitors have obtained accelerated approval for treating KRAS^(G12C)mutant lung cancer,and allele-specific hotspot mutations on G12D/S/R have been demonstrated as viable targets.New methods of targeting KRAS are quickly evolving,including transcription,immunogenic neoepitopes,and combinatory targeting with immunotherapy.Nevertheless,the vast majority of small GTPases and hotspot mutations remain elusive,and clinical resistance to G12C inhibitors poses new challenges.In this article,we summarize diversified biological functions,shared structural properties,and complex regulatory mechanisms of small GTPases and their relationships with human diseases.Furthermore,we review the status of drug discovery for targeting small GTPases and the most recent strategic progress focused on targeting KRAS.The discovery of new regulatory mechanisms and development of targeting approaches will together promote drug discovery for small GTPases.展开更多
Inflammatory bowel disease(IBD) is a chronic lifelong disease characterized by inflammation of the gastrointestinal tract.Although more and more treatment options serve IBD, there is still no cure. It is important to ...Inflammatory bowel disease(IBD) is a chronic lifelong disease characterized by inflammation of the gastrointestinal tract.Although more and more treatment options serve IBD, there is still no cure. It is important to find an effective treatment for IBD.This study aims to investigate whether Lactobacillus plantarum(L. plantarum) could alleviate colitis induced by dextran sulfate sodium(DSS). Following the DSS challenge, L. plantarum on DSS-mediated inflammatory colon lesions in mice, and L.plantarum therapy heightened the relative abundance of the colon-resident Actinobacteria. Analysis of serum metabolomics also indicated that the content of MG(18:4(6 Z, 9 Z, 12 Z, 15 Z)/0:0/0:0) was increased in response to L. plantarum therapy, and this was also the case for indolepyruvate and 1-hydroxyibuprofen. However, 13-oxooctadecadienoic acid(13-oxo ODE) and indolylacryloylglycine content fell following the DSS challenge. Based on these results, the study elucidates the mitigatory effects of L. plantarum in colitis, which depend on its regulation of the colonic microbial community and its modification of serum metabolites. The results revealed that L. plantarum mitigated inflammatory colon lesions, reprogrammed the microbial community and altered the level of serum metabolites in a murine model challenged with DSS. The study may present a potential therapeutic strategy for colitis.展开更多
With the rapid development of sequencing technology,research on pigs has focused on intestinal microbes.Accumulating evidence suggests that the metabolites of intestinal microbes are the key medium for interactions be...With the rapid development of sequencing technology,research on pigs has focused on intestinal microbes.Accumulating evidence suggests that the metabolites of intestinal microbes are the key medium for interactions between microbes and the host.Amino acid metabolism is involved in the growth and immune processes of pigs.The gut microbes of pigs are heavily involved in the metabolism of amino acids in their hosts.Here,we review the latest relevant literature.Research findings show that microbial metabolites,such as indoles,short-chain fatty acids,and ammonia,play a key role in gut health.Moreover,we summarize the effects of amino acids on the structure of the gut microbial community and the metabolism of amino acids by pig gut microbes.Evidence shows that microbial amino acid metabolites act as signal molecules in the intestine and play an important role in the intestinal health of pigs.展开更多
文摘目的探讨与肝细胞癌(HCC)远处转移相关的DNA拷贝数变异(copy number alteration,CNA)分子标志。方法采用高分辨率Agilent 244K微阵列比较基因组杂交(aCGH)方法检测63例HCC样本基因组DNA的CNA特征。以Log-rank检验、Kaplan-Meier生存分析以及Cox风险比例模型,分析各DNA片段CNA与HCC远处转移的相关性。结果染色体片段12p12.2-13.31丢失是HCC患者远处转移的显著危险因素(P<0.01,Log-rank检验)。与非丢失者相比,12p12.2-13.31丢失HCC患者的远处转移风险比(hazard ratio,HR)为22.98(95%CI=4.29~123.22,P<0.01)。多变量Cox回归分析显示,12p12.2-13.31丢失是HCC远处转移的独立预测因素(HR=7.94,95%CI=1.14~55.61,P<0.05)。结论染色体片段12p12.2-13.31丢失增加HCC远处转移风险,可作为预测HCC远处转移的一个分子标志。
基金supported by the National Natural Science Foundation of China (31772642, 31672457)Ministry of Agricultural of the People’s Republic of China (2015-Z64, 2016-X47)+4 种基金Hunan Provincial Science and Technology Department (2021J30008, 2019TP2004,2017NK2322, 2016WK2008, 2016TP2005)Double first-class construction project of Hunan Agricultural University (SYL201802003)China Postdoctoral Science Foundation (2018M632963, 2019T120705)Postgraduate Scientific Research Innovation Project of Hunan Province (CX20210654)Science and Technology Innovation and Entrepreneurship Project for University Students of Hunan Province (2021RC1004)。
文摘Trace metal elements, such as iron, copper, manganese, and zinc, are essential nutrients for biological processes. Although their intake demand is low, they play a crucial role in cell homeostasis as the cofactors of various enzymes. Symbiotic intestinal microorganisms compete with their host for the use of trace metal elements. Moreover, the metabolic processes of trace metal elements in the host and microorganisms affect the organism's health. Supplementation or the lack of trace metal elements in the host can change the intestinal microbial community structure and function. Functional changes in symbiotic microorganisms can affect the host's metabolism of trace metal elements. In this review, we discuss the absorption and transport processes of trace metal elements in the host and symbiotic microorganisms and the effects of dynamic changes in the levels of trace metal elements on the intestinal microbial community structure. We also highlight the participation of trace metal elements as enzyme cofactors in the host immune process. Our findings indicate that the host uses metal nutrition immunity or metal poisoning to resist pathogens and improve immunity.
基金supported by grants from National Key R&D Program of China(2022YFC2305400,2022YFC3400600)Shenzhen Fundamental Research Program(JCYJ20220530145011025)+1 种基金National Natural ScienceFoundation of China(82282717,82073311)Natural Science Foundation of Sichuan Province(2022JDTD0025)。
文摘Small GTPases including Ras,Rho,Rab,Arf,and Ran are omnipresent molecular switches in regulating key cellular functions.Their dysregulation is a therapeutic target for tumors,neurodegeneration,cardiomyopathies,and infection.However,small GTPases have been historically recognized as“undruggable”.Targeting KRAS,one of the most frequently mutated oncogenes,has only come into reality in the last decade due to the development of breakthrough strategies such as fragment-based screening,covalent ligands,macromolecule inhibitors,and PROTACs.Two KRAS^(G12C)covalent inhibitors have obtained accelerated approval for treating KRAS^(G12C)mutant lung cancer,and allele-specific hotspot mutations on G12D/S/R have been demonstrated as viable targets.New methods of targeting KRAS are quickly evolving,including transcription,immunogenic neoepitopes,and combinatory targeting with immunotherapy.Nevertheless,the vast majority of small GTPases and hotspot mutations remain elusive,and clinical resistance to G12C inhibitors poses new challenges.In this article,we summarize diversified biological functions,shared structural properties,and complex regulatory mechanisms of small GTPases and their relationships with human diseases.Furthermore,we review the status of drug discovery for targeting small GTPases and the most recent strategic progress focused on targeting KRAS.The discovery of new regulatory mechanisms and development of targeting approaches will together promote drug discovery for small GTPases.
基金This study was supported by the National Natural Science Foundation of China(31772642,31672457)Hunan Provincial Science and Technology Department(2019TP2004,2017NK2322,2016WK2008,2016TP2005)+1 种基金Double first-class construction project of Hunan Agricultural University(SYL201802003)China Postdoctoral Science Foundation(2018M632963,2019T120705).
文摘Inflammatory bowel disease(IBD) is a chronic lifelong disease characterized by inflammation of the gastrointestinal tract.Although more and more treatment options serve IBD, there is still no cure. It is important to find an effective treatment for IBD.This study aims to investigate whether Lactobacillus plantarum(L. plantarum) could alleviate colitis induced by dextran sulfate sodium(DSS). Following the DSS challenge, L. plantarum on DSS-mediated inflammatory colon lesions in mice, and L.plantarum therapy heightened the relative abundance of the colon-resident Actinobacteria. Analysis of serum metabolomics also indicated that the content of MG(18:4(6 Z, 9 Z, 12 Z, 15 Z)/0:0/0:0) was increased in response to L. plantarum therapy, and this was also the case for indolepyruvate and 1-hydroxyibuprofen. However, 13-oxooctadecadienoic acid(13-oxo ODE) and indolylacryloylglycine content fell following the DSS challenge. Based on these results, the study elucidates the mitigatory effects of L. plantarum in colitis, which depend on its regulation of the colonic microbial community and its modification of serum metabolites. The results revealed that L. plantarum mitigated inflammatory colon lesions, reprogrammed the microbial community and altered the level of serum metabolites in a murine model challenged with DSS. The study may present a potential therapeutic strategy for colitis.
基金National Natural Science Foundation of China(No.31772642,31672457,41807135)Hunan Provincial Science and Technology Department(2019TP2004,2020NK2004,2020ZL2004)+4 种基金China Postdoctoral Science Foundation(2018M632963,2019T120705)Scientific Research Fund of Hunan Provincial Education Department(2020JGYB112,18B107)Double First-class Construction Project of Hunan Agricultural University(SYL201802003,YB2018007,CX20190497)Natural Science Foundation of Hunan province,China(No.2019JJ50220)Undergraduate on Innovation and Entrepreneurship Training Program(No.S202010537084).
文摘With the rapid development of sequencing technology,research on pigs has focused on intestinal microbes.Accumulating evidence suggests that the metabolites of intestinal microbes are the key medium for interactions between microbes and the host.Amino acid metabolism is involved in the growth and immune processes of pigs.The gut microbes of pigs are heavily involved in the metabolism of amino acids in their hosts.Here,we review the latest relevant literature.Research findings show that microbial metabolites,such as indoles,short-chain fatty acids,and ammonia,play a key role in gut health.Moreover,we summarize the effects of amino acids on the structure of the gut microbial community and the metabolism of amino acids by pig gut microbes.Evidence shows that microbial amino acid metabolites act as signal molecules in the intestine and play an important role in the intestinal health of pigs.