in hippocampal neurons is an important contributor to depression. [Ca^2＋]i of hippocampal neurons may raise rapidly with increased corticosterone concentrations, leading to apoptosis. The anti-depressant effects of X...in hippocampal neurons is an important contributor to depression. [Ca^2＋]i of hippocampal neurons may raise rapidly with increased corticosterone concentrations, leading to apoptosis. The anti-depressant effects of Xiaoyaosan （Free and Easy Wanderer＇s Powder） may inhibit apoptosis by down regulating the [Ca^2＋]i overload. PC12 cells were containing with corticosterone to simulate neuronal injury, dosed with Xiaoyaosan-containing serum, and the following parameters were measured： apoptosis,[Ca^2＋]i overload, and changes in mitochondrial membrane potential （△ψm） and bioenergetics. High-dose Xiaoyaosan-containing serum （the dose of intragastric administration was 2.633 g herb/mL） not only inhibited morphological injury and cell apoptosis, but also suppressed [Ca^2＋]i overload induced by corticosterone. Xiaoyaosan also blocked the decrease in mitochondrial membrane potential and adenosine triphosphate levels induced by corticosterone. Xiaoyaosan dose-dependently suppresses corticosterone-induced apoptosis, potentially via inhibiting [Ca^2＋]ioverload, and prevents impaired mitochondrial bioenergetics by maintaining mitochondrial membrane potential and adenosine triphosphate production, providing a putative mechanism for its antidepressant effects.展开更多
基金a Grant from the Ministry of Education of the People’s Republic of China,No.NCET-05-0750a Grant from Guangdong Provincial Natural Science Research Team,No.5200491
文摘in hippocampal neurons is an important contributor to depression. [Ca^2＋]i of hippocampal neurons may raise rapidly with increased corticosterone concentrations, leading to apoptosis. The anti-depressant effects of Xiaoyaosan （Free and Easy Wanderer＇s Powder） may inhibit apoptosis by down regulating the [Ca^2＋]i overload. PC12 cells were containing with corticosterone to simulate neuronal injury, dosed with Xiaoyaosan-containing serum, and the following parameters were measured： apoptosis,[Ca^2＋]i overload, and changes in mitochondrial membrane potential （△ψm） and bioenergetics. High-dose Xiaoyaosan-containing serum （the dose of intragastric administration was 2.633 g herb/mL） not only inhibited morphological injury and cell apoptosis, but also suppressed [Ca^2＋]i overload induced by corticosterone. Xiaoyaosan also blocked the decrease in mitochondrial membrane potential and adenosine triphosphate levels induced by corticosterone. Xiaoyaosan dose-dependently suppresses corticosterone-induced apoptosis, potentially via inhibiting [Ca^2＋]ioverload, and prevents impaired mitochondrial bioenergetics by maintaining mitochondrial membrane potential and adenosine triphosphate production, providing a putative mechanism for its antidepressant effects.
