Neurodegenerative disorders affect millions of people worldwide,and the prevalence of these disorders is only projected to rise as the number of people over 65 will drastically increase in the coming years.While thera...Neurodegenerative disorders affect millions of people worldwide,and the prevalence of these disorders is only projected to rise as the number of people over 65 will drastically increase in the coming years.While therapies exist to aid in symptomatic relief,effective treatments that can stop or reve rse the progress of each neurodegenerative disease are lacking.Recently,research on the role of extracellular vesicles as disease markers and therapeutics has been intensively studied.Exosomes,30-150 nm in diameter,are one type of extracellular vesicles facilitating cell-to-cell communication.Exosomes are thought to play a role in disease propagation in a variety of neurodegenerative diseases,such as Alzheimer's disease,Parkinson s disease,and amyotrophic lateral sclerosis.Accordingly,the exosomes derived from the patients are an invaluable source of disease biomarkers.On the other hand,exosomes,especially those derived from stem cells,could serve as a therapeutic for these disorders,as seen by a rapid increase in clinical trials investigating the therapeutic efficacy of exosomes in different neurological diseases.This review summarizes the pathological burden and therapeutic approach of exosomes in neurodegenerative disorders.We also highlight how heat shock increases the yield of exosomes while still maintaining their therapeutic efficacy.Finally,this review concludes with outstanding questions that remain to be addressed in exosomal research.展开更多
Ischemic stroke is a global epidemic condition due to an inadequate supply of blood and oxygen to a specific area of brain either by arterial blockage or by narrowing of blood vessels.Despite having advancement in the...Ischemic stroke is a global epidemic condition due to an inadequate supply of blood and oxygen to a specific area of brain either by arterial blockage or by narrowing of blood vessels.Despite having advancement in the use of thrombolytic and clot removal medicine,significant numbers of stroke patients are still left out without option for treatment.In this review,we summarize recent research work on the activation ofδ-opioid receptor as a strategy for treating ischemic stroke-caused neuronal injury.Moreover,as activation ofδ-opioid receptor by a non-peptidicδ-opioid receptor agonist also modulates the expression,maturation and processing of amyloid precursor protein andβ-secretase activity,the potential role of these effects on ischemic stroke caused dementia or Alzheimer’s disease are also discussed.展开更多
A Pt-MoO3/C catalyst,aimed to eliminate the harmful effect of sulfur dioxide（SCb） on the performance of Pt nanoparticles（NPs） for catalysis of oxygen reduction reaction（ORR） in proton exchange membrane fuel cell...A Pt-MoO3/C catalyst,aimed to eliminate the harmful effect of sulfur dioxide（SCb） on the performance of Pt nanoparticles（NPs） for catalysis of oxygen reduction reaction（ORR） in proton exchange membrane fuel cells（PEMFC）,is developed and characterized by TEM,XRD and XPS.The results reveal that Pt-MoO3/C catalyst exhibits not only a higher catalytic activity,but also a better SO2 poisoning resistance and a better recovery performance than the commercial Pt/C catalyst does.展开更多
OBJECTIVE To observe the effect of curcumin on proliferation and apop-tosis in the prostate cancer LNCaP cell line. METHODS The AXSYMTM system luciferase method was used to examine the effect of various concentratious...OBJECTIVE To observe the effect of curcumin on proliferation and apop-tosis in the prostate cancer LNCaP cell line. METHODS The AXSYMTM system luciferase method was used to examine the effect of various concentratious of curcumin on the content of prostate specific antigen (PSA) in prostate cancer LNCaP cells. A pGL3-PSA luciferase expression vector, containing 640 bp DNA of the PSA gene 5' -promoter region was constructed and transfected into the LNCaP cells with lipofectin. By measuring luciferase activity, the effect of 10 μmol/L, 20 μmol/L, 30 and 40 μmol/L curcumin on the promoter was studied. Effects on cell growth and apoptosis were analyzed by microscopy, the MTT colorimetric assay and flow cytometry. Western-blotting was used to measure expression of the androgen receptor (AR) in the LNCaP cells treated with different concentrations of curcumin. RESULTS The results showed that the expression of PSA was inhibited as curcumin reduced the activity of luciferase. Curcumin also caused a sigificant concentration-dependent decrease in AR expession measured by Western -blotting. Cell growth was inhibited and apoptosis was induced. CONCLUSION By inhibiting AR expression, curcumin reduced the function of the PSA promoter and inhibited PSA protein expression. Curcumin decreased the cellular proliferation and induced apoptosis in LNCaP cells in a concention-dependent manner.展开更多
Covalent organic frameworks（COFs） represent an emerging class of porous crystalline materials and have recently shown interesting applications from catalysis to optoelectronic devices.In this review,by covering most...Covalent organic frameworks（COFs） represent an emerging class of porous crystalline materials and have recently shown interesting applications from catalysis to optoelectronic devices.In this review,by covering most of the reported work,we summarized the research progress of two-dimensional（2D）porphyrin- and phthalocyanine-based COFs,with highlighting the synthesis of these 2D COFs via various dynamic covalent reactions and emphasizing their potential applications in different areas.展开更多
The evolution of the Changjiang Delta is obviously affected by current rapidly rising sea level and drastically declining river discharge,and it is also vital for the sustainable development of Shanghai and the Changj...The evolution of the Changjiang Delta is obviously affected by current rapidly rising sea level and drastically declining river discharge,and it is also vital for the sustainable development of Shanghai and the Changjiang River Economic Belt,which represent China’s economic development leader and major national strategic area,respectively.In this paper,the growth pattern of Jiuduansha Island,the largest uninhabited alluvial island in the Changjiang Estuary,is studied in terms of the change in elevation,position and area over the past 50 years through using satellite-derived instantaneous shoreline positions and high/low tide exposure areas based on 497 satellite images from 1974 to 2020;and the influencing factors and future development patterns are further discussed by comparison with other alluvial islands or sandbars in the estuary.The results show that Jiuduansha Island has maintained a rapid or even accelerated area growth rate,although the sediment discharge of the Changjiang River has sharply decreased in recent decades.This sustained growth is mainly attributed to the existence of the estuarine turbidity maximum zone,coarsening fluvial sediment,onshore sediment replenishment by tide,cone-like geomorphology of Jiuduansha Island,and siltation promotion effect of vegetation.The growth rate of the low tide exposure area of Jiuduansha Island increased from 1.9 km^(2)a^(−1) in 1974–1990 to 3.0 km^(2)a^(−1) in 1990–2020,and the growth rate of the high tide exposure area reached as high as 3.7 km^(2)a^(−1) in 1994–2020.The implementation of the Deep-Water Channel Project has significantly affected the evolution of Jiuduansha Island,including shifting the heads of Shangsha and Zhongxiasha from severe retreat to rapid accretion,and promoting tidal flat progradation seaward of the Jiangyanansha and Zhongxiasha,but restricting the growth of the low tide exposure area of Jiuduansha Island.展开更多
基金supported by the National Institute on Aging of NIH(No.RF1AG072510 to HW)the National Institute of General Medical Sciences(NINGM)of NIH(No.P20GM103443 to HW via Dr.Victor Huber)+1 种基金the National Science Foundation(NSF)(No.DGE-1633213 to CCH via Dr.Brian Burrell)the NIH/NIGMS(No.T32GM-136503 to CCH via Dr.Brian Burrell)。
文摘Neurodegenerative disorders affect millions of people worldwide,and the prevalence of these disorders is only projected to rise as the number of people over 65 will drastically increase in the coming years.While therapies exist to aid in symptomatic relief,effective treatments that can stop or reve rse the progress of each neurodegenerative disease are lacking.Recently,research on the role of extracellular vesicles as disease markers and therapeutics has been intensively studied.Exosomes,30-150 nm in diameter,are one type of extracellular vesicles facilitating cell-to-cell communication.Exosomes are thought to play a role in disease propagation in a variety of neurodegenerative diseases,such as Alzheimer's disease,Parkinson s disease,and amyotrophic lateral sclerosis.Accordingly,the exosomes derived from the patients are an invaluable source of disease biomarkers.On the other hand,exosomes,especially those derived from stem cells,could serve as a therapeutic for these disorders,as seen by a rapid increase in clinical trials investigating the therapeutic efficacy of exosomes in different neurological diseases.This review summarizes the pathological burden and therapeutic approach of exosomes in neurodegenerative disorders.We also highlight how heat shock increases the yield of exosomes while still maintaining their therapeutic efficacy.Finally,this review concludes with outstanding questions that remain to be addressed in exosomal research.
基金supported by the National Institute of Neurological Disorders and Stroke under research grant NS088084(to HW)
文摘Ischemic stroke is a global epidemic condition due to an inadequate supply of blood and oxygen to a specific area of brain either by arterial blockage or by narrowing of blood vessels.Despite having advancement in the use of thrombolytic and clot removal medicine,significant numbers of stroke patients are still left out without option for treatment.In this review,we summarize recent research work on the activation ofδ-opioid receptor as a strategy for treating ischemic stroke-caused neuronal injury.Moreover,as activation ofδ-opioid receptor by a non-peptidicδ-opioid receptor agonist also modulates the expression,maturation and processing of amyloid precursor protein andβ-secretase activity,the potential role of these effects on ischemic stroke caused dementia or Alzheimer’s disease are also discussed.
基金supported by China National 973 Program(2012CB720300)NSFC of China(Grant No.21276291 and 21176327)
文摘A Pt-MoO3/C catalyst,aimed to eliminate the harmful effect of sulfur dioxide（SCb） on the performance of Pt nanoparticles（NPs） for catalysis of oxygen reduction reaction（ORR） in proton exchange membrane fuel cells（PEMFC）,is developed and characterized by TEM,XRD and XPS.The results reveal that Pt-MoO3/C catalyst exhibits not only a higher catalytic activity,but also a better SO2 poisoning resistance and a better recovery performance than the commercial Pt/C catalyst does.
文摘OBJECTIVE To observe the effect of curcumin on proliferation and apop-tosis in the prostate cancer LNCaP cell line. METHODS The AXSYMTM system luciferase method was used to examine the effect of various concentratious of curcumin on the content of prostate specific antigen (PSA) in prostate cancer LNCaP cells. A pGL3-PSA luciferase expression vector, containing 640 bp DNA of the PSA gene 5' -promoter region was constructed and transfected into the LNCaP cells with lipofectin. By measuring luciferase activity, the effect of 10 μmol/L, 20 μmol/L, 30 and 40 μmol/L curcumin on the promoter was studied. Effects on cell growth and apoptosis were analyzed by microscopy, the MTT colorimetric assay and flow cytometry. Western-blotting was used to measure expression of the androgen receptor (AR) in the LNCaP cells treated with different concentrations of curcumin. RESULTS The results showed that the expression of PSA was inhibited as curcumin reduced the activity of luciferase. Curcumin also caused a sigificant concentration-dependent decrease in AR expession measured by Western -blotting. Cell growth was inhibited and apoptosis was induced. CONCLUSION By inhibiting AR expression, curcumin reduced the function of the PSA promoter and inhibited PSA protein expression. Curcumin decreased the cellular proliferation and induced apoptosis in LNCaP cells in a concention-dependent manner.
基金supported by National Natural Science Foundation of China(No.21572170)the Research Fund for the Doctoral Program of Higher Education of China(No.20130141110008)+2 种基金the Outstanding Youth Foundation of Hubei Province(No.2015CFA045)the Beijing National Laboratory for Molecular Sciencesthe Open Foundation of State Key Laboratory of Electronic Thin Films and Integrated Devices(No.KFJJ201505)
文摘Covalent organic frameworks（COFs） represent an emerging class of porous crystalline materials and have recently shown interesting applications from catalysis to optoelectronic devices.In this review,by covering most of the reported work,we summarized the research progress of two-dimensional（2D）porphyrin- and phthalocyanine-based COFs,with highlighting the synthesis of these 2D COFs via various dynamic covalent reactions and emphasizing their potential applications in different areas.
基金supported by the National Natural Science Foundation of China(Grant No.41776052)Shandong Provincial Natural Science Foundation,China(Grant No.ZR2019MD037).
文摘The evolution of the Changjiang Delta is obviously affected by current rapidly rising sea level and drastically declining river discharge,and it is also vital for the sustainable development of Shanghai and the Changjiang River Economic Belt,which represent China’s economic development leader and major national strategic area,respectively.In this paper,the growth pattern of Jiuduansha Island,the largest uninhabited alluvial island in the Changjiang Estuary,is studied in terms of the change in elevation,position and area over the past 50 years through using satellite-derived instantaneous shoreline positions and high/low tide exposure areas based on 497 satellite images from 1974 to 2020;and the influencing factors and future development patterns are further discussed by comparison with other alluvial islands or sandbars in the estuary.The results show that Jiuduansha Island has maintained a rapid or even accelerated area growth rate,although the sediment discharge of the Changjiang River has sharply decreased in recent decades.This sustained growth is mainly attributed to the existence of the estuarine turbidity maximum zone,coarsening fluvial sediment,onshore sediment replenishment by tide,cone-like geomorphology of Jiuduansha Island,and siltation promotion effect of vegetation.The growth rate of the low tide exposure area of Jiuduansha Island increased from 1.9 km^(2)a^(−1) in 1974–1990 to 3.0 km^(2)a^(−1) in 1990–2020,and the growth rate of the high tide exposure area reached as high as 3.7 km^(2)a^(−1) in 1994–2020.The implementation of the Deep-Water Channel Project has significantly affected the evolution of Jiuduansha Island,including shifting the heads of Shangsha and Zhongxiasha from severe retreat to rapid accretion,and promoting tidal flat progradation seaward of the Jiangyanansha and Zhongxiasha,but restricting the growth of the low tide exposure area of Jiuduansha Island.
