Nanozymes based on metal-organic frameworks (MOFs) have been concentrated on due to their naturally high-disperse metal active sites and the adjustable coordination chemistry. In this work, an N-rich melamine (Mel) wa...Nanozymes based on metal-organic frameworks (MOFs) have been concentrated on due to their naturally high-disperse metal active sites and the adjustable coordination chemistry. In this work, an N-rich melamine (Mel) was introduced into the Cu-MOF composed of copper(II) nitrate and 2-aminoterephthalic acid (Cu-NH_(2)-BDC-Mel) to mimic the laccase, which enzyme-like activities were assessed and applied in sensing analyses toward several phenols and amines. Compared to unmodified Cu-NH_(2)-BDC, the resulting Cu-NH_(2)-BDC-Mel exhibits enhanced laccase-like activity, superior stability and catalytic kinetics. It is demonstrated that melamine-doping has increased nitrogen content as well as the surface area, as a result, exhibits a lower Michaelis–Menten constant (K m) (0.1877 mM) and higher maximum reaction rate (V max) (1.7933 × 10^(−3) mM·min^(−1)) in comparison with that of natural laccase. Based on that, an efficient colorimetric sensing strategy for several phenols and amines was built up with excellent selectivity and anti-interference by using the laccase-like Cu-NH_(2)-BDC-Mel, the detection limits are 3.51 µM of adrenaline and 4.41 µM of dopamine. The work broadens the prospect development of bio-colorimetric sensing based on the ligand-modified Cu-MOFs nanozymes catalysis.展开更多
基金financial support of Liaoning Science and Technology Development Foundation Guided by Central Government(No.2021JH6/10500141)Fundamental Research Funds for the Central Universities(No.N232410019).
文摘Nanozymes based on metal-organic frameworks (MOFs) have been concentrated on due to their naturally high-disperse metal active sites and the adjustable coordination chemistry. In this work, an N-rich melamine (Mel) was introduced into the Cu-MOF composed of copper(II) nitrate and 2-aminoterephthalic acid (Cu-NH_(2)-BDC-Mel) to mimic the laccase, which enzyme-like activities were assessed and applied in sensing analyses toward several phenols and amines. Compared to unmodified Cu-NH_(2)-BDC, the resulting Cu-NH_(2)-BDC-Mel exhibits enhanced laccase-like activity, superior stability and catalytic kinetics. It is demonstrated that melamine-doping has increased nitrogen content as well as the surface area, as a result, exhibits a lower Michaelis–Menten constant (K m) (0.1877 mM) and higher maximum reaction rate (V max) (1.7933 × 10^(−3) mM·min^(−1)) in comparison with that of natural laccase. Based on that, an efficient colorimetric sensing strategy for several phenols and amines was built up with excellent selectivity and anti-interference by using the laccase-like Cu-NH_(2)-BDC-Mel, the detection limits are 3.51 µM of adrenaline and 4.41 µM of dopamine. The work broadens the prospect development of bio-colorimetric sensing based on the ligand-modified Cu-MOFs nanozymes catalysis.
