Nonalcoholic fatty liver disease (NAFLD) is considered a major health epidemic with an estimated 32.4% worldwide prevalence. No drugs have yet been approved and therapeutic nodes remain a major unmet need. Long noncod...Nonalcoholic fatty liver disease (NAFLD) is considered a major health epidemic with an estimated 32.4% worldwide prevalence. No drugs have yet been approved and therapeutic nodes remain a major unmet need. Long noncoding RNAs are emerging as an important class of novel regulators influencing multiple biological processes and the pathogenesis of NAFLD. Herein, we described a novel long noncoding RNA, lnc_217, which was liver enriched and upregulated in high-fat diet-fed mice, and a genetic animal model of NAFLD. We found that liver specific knockdown of lnc_217 was resistant to high-fat diet-induced hepatic lipid accumulation and decreased serum lipid in mice. Mechanistically, we demonstrated that knockdown of lnc_217 not only decreased de novo lipogenesis by inhibiting sterol regulatory element binding protein-1c cleavage but also increased fatty acid β- oxidation through activation of peroxisome proliferator-activated receptor α and carnitine palmitoyltransferase-1α. Taken together, we conclude that lnc_217 may be a novel regulator of hepatic lipid metabolism and a potential therapeutic target for the treatment of hepatic steatosis and NAFLD-related metabolic disorders.展开更多
We sought to assess the psychological status and diabetes-related distress of Chinese type 1 diabetes patients in Jiangsu province, China. According to the World Health Organization criteria, 42 patients with type 1 d...We sought to assess the psychological status and diabetes-related distress of Chinese type 1 diabetes patients in Jiangsu province, China. According to the World Health Organization criteria, 42 patients with type 1 diabetes were enrolled in the study and assessed with the Self-Rating Anxiety Scale （SAS）, Self-Rating Depression Scale （SDS） and Diabetes Distress Scale （DDS）. All data were tabulated and statistical analyses were performed. The study summarized cases of 42 patients with type 1 diabetes, including 17 males and 25 females with a mean age of 23- 12 years and a mean duration of diabetes of 7_ 6 years. Compared to the Chinese normative data, the SAS standard score was significantly higher, whereas SDS standard score had no statistical significance. The SAS standard score was most highly correlated with diabetes duration （y = 0.547, P = 0.011）. Additionally, 19.5% of the patients had moderate or even severe diabetes-related distress and 21.4% had moderate or even severe emo- tional burden while 26.2% had regimen-related distress. Multiple stepwise regression analysis showed that the mean correlation between DDS and the four domains was high, particularly the emotional burden domain （esti- mated D = 0.363, P 〈 0.001） and regimen-related distress domain （estimated 13 = 0.356, P 〈 0.001）. The correlation between SAS and DDS was positive （estimated 13 = 0.039, P = 0.027）. In conclusion, the results showed the im- portance of psychological aspects in Chinese individuals with type 1 diabetes. Screening and treatment of psycho- logical aspects may result in better adherence and increased quality of life for patients with diabetes.展开更多
Multiple endocrine neoplasia type 2A （MEN2A）, a subtype of MEN2, is characterized by medullary thyroid cancer, pheochromocytoma, and primary hyperparathyroidism. A Han Chinese pedigree with MEN2A was investigated fo...Multiple endocrine neoplasia type 2A （MEN2A）, a subtype of MEN2, is characterized by medullary thyroid cancer, pheochromocytoma, and primary hyperparathyroidism. A Han Chinese pedigree with MEN2A was investigated following confirmation of the proband＇s diagnosis by pathological findings and DNA/biochemical screening. DNA samples from 4 other family members were collected and exon 5, 8, 10, 11, 13, 16 and 18 of the RET proto-oncogene were sequenced and then analyzed. A missense mutation of TGG （Trp） to TGC （Cys） at codon 634 （the classic MEN2A mutation） in exon 11 of the RET gene was detected in 3 family members, including the proband. Sequencing data were compared with the human gene mutation database. Elevated serum calcitonin level was detected initially; medullary thyroid carcinoma was revealed in the 3 cases and adrenal pheochromocytoma was also found in the proband. Elective operations were successfully performed on the adrenal and thyroid glands because of pheochromocytoma and medullary thyroid carcinoma. Our case study confirms that integrated DNA-based/biochemical screening is crucial for early diagnosis of MEN2A and is helpful in the screening of their relatives. In addition, DNA-based screening may occasionally uncover a previously unknown RET sequence.展开更多
The low degree of development and utilization as well as the contradiction between supply and demand of water resources in Huangshui River basin are the main restricting factors of the local agricultural development. ...The low degree of development and utilization as well as the contradiction between supply and demand of water resources in Huangshui River basin are the main restricting factors of the local agricultural development. The study on the simulation of irrigation water loss based on the VSMB model has very important significance to strengthening regional water management and improving water resource utilization efficiency. Five groundwater wells were set up to carry out the farmland irrigation water infiltration and the experimental study on groundwater dynamic effect. Two soil moisture monitoring sites were set up in two typical plots of Daxia and Guanting irrigation area at the same time and TDR300 was used to monitor four kinds of deep soil moisture( 10 cm,30 cm,50 cm and 70 cm). On this basis,the VSMB model was used to study the irrigation water loss in the irrigation area of Yellow River valley of Qinghai Province,including soil moisture content,the actual evapotranspiration,infiltration,runoff,groundwater buried depth and so on. The results showed that the water consumption caused by soil evaporation and crop transpiration accounted for 46. 4% and 24. 1% of the total precipitation plus irrigation,respectively,and the leakage accounted for 30. 3% and 60. 6% of the total precipitation plus irrigation,respectively,from March 1,2013 to April 30,and from August 1 to September 30. The actual evaporation of the GT- TR1 and GT- TR2 sites in the whole year of 2013 was 632. 6 mm and 646. 9 mm,respectively,and the leakage accounted for 2. 6% and 1. 2% of the total precipitation plus irrigation,respectively. RMSE of the simulation results of the groundwater depth in Daxia irrigation area during the two periods was 92. 3 mm and 27. 7 mm,respectively. And RMSE of the simulation results of the water content of soil profile in the two monitoring sites of Guanting irrigation area was 2. 04% and 5. 81%,respectively,indicating that the simulation results were reliable.展开更多
<div style="text-align:justify;"> A new planar waveguide laser was demonstrated. The output energy of 400 mW was achieved by a single waveguide laser;the slope efficiency was 61%. The single waveguide ...<div style="text-align:justify;"> A new planar waveguide laser was demonstrated. The output energy of 400 mW was achieved by a single waveguide laser;the slope efficiency was 61%. The single waveguide laser can expand to waveguide laser group and waveguide laser array to produce higher energy. </div>展开更多
Type 1 diabetes mellitus is heterogeneous in many facets. The patients suffered from type 1 diabetes present several levels of islet function as well as variable number and type of islet-specific autoantibodies. This ...Type 1 diabetes mellitus is heterogeneous in many facets. The patients suffered from type 1 diabetes present several levels of islet function as well as variable number and type of islet-specific autoantibodies. This study was to investigate prevalence and heterogeneity of the islet autoantibodies and clinical phenotypes of type 1 diabetes mellitus; and also discussed the process of islet failure and its risk factors in Chinese type 1 diabetic patients. A total of 1,291 type 1 diabetic patients were enrolled in this study. Demographic information was collected. Laboratory tests including mixed-meal tolerance test, human leukocyte antigen alleles, hemoglobin A1 c, lipids, thyroid function and islet autoantibodies were conducted. The frequency of islet-specific autoantibody in newly diagnosed T1 DM patients(duration shorter than half year) was 73% in East China. According to binary logistic regressions, autoantibody positivity, longer duration and lower Body Mass Index were the risk factors of islet failure. As the disease developed, autoantibodies against glutamic acid decarboxylase declined as well as the other two autoantibodies against zinc transporter 8 and islet antigen 2. The decrease of autoantibodies was positively correlated with aggressive beta cell destruction. Autoantibodies can facilitate the identification of classic T1 DM from other subtypes and predict the progression of islet failure. As there were obvious heterogeneity in autoantibodies and clinical manifestation in different phenotypes of the disease, we should take more factors into consideration when identifying type 1 diabetes mellitus.展开更多
基金This work was supported by grants from the National Natural Science Foundation of China(Grant Nos.32130050,32201064,and 82170838)the Natural Science Research Project of Universities in Jiangsu Province(Grant No.21KJB180003).
文摘Nonalcoholic fatty liver disease (NAFLD) is considered a major health epidemic with an estimated 32.4% worldwide prevalence. No drugs have yet been approved and therapeutic nodes remain a major unmet need. Long noncoding RNAs are emerging as an important class of novel regulators influencing multiple biological processes and the pathogenesis of NAFLD. Herein, we described a novel long noncoding RNA, lnc_217, which was liver enriched and upregulated in high-fat diet-fed mice, and a genetic animal model of NAFLD. We found that liver specific knockdown of lnc_217 was resistant to high-fat diet-induced hepatic lipid accumulation and decreased serum lipid in mice. Mechanistically, we demonstrated that knockdown of lnc_217 not only decreased de novo lipogenesis by inhibiting sterol regulatory element binding protein-1c cleavage but also increased fatty acid β- oxidation through activation of peroxisome proliferator-activated receptor α and carnitine palmitoyltransferase-1α. Taken together, we conclude that lnc_217 may be a novel regulator of hepatic lipid metabolism and a potential therapeutic target for the treatment of hepatic steatosis and NAFLD-related metabolic disorders.
基金supported by Natural Science Foundation of China(No.81070622)
文摘We sought to assess the psychological status and diabetes-related distress of Chinese type 1 diabetes patients in Jiangsu province, China. According to the World Health Organization criteria, 42 patients with type 1 diabetes were enrolled in the study and assessed with the Self-Rating Anxiety Scale （SAS）, Self-Rating Depression Scale （SDS） and Diabetes Distress Scale （DDS）. All data were tabulated and statistical analyses were performed. The study summarized cases of 42 patients with type 1 diabetes, including 17 males and 25 females with a mean age of 23- 12 years and a mean duration of diabetes of 7_ 6 years. Compared to the Chinese normative data, the SAS standard score was significantly higher, whereas SDS standard score had no statistical significance. The SAS standard score was most highly correlated with diabetes duration （y = 0.547, P = 0.011）. Additionally, 19.5% of the patients had moderate or even severe diabetes-related distress and 21.4% had moderate or even severe emo- tional burden while 26.2% had regimen-related distress. Multiple stepwise regression analysis showed that the mean correlation between DDS and the four domains was high, particularly the emotional burden domain （esti- mated D = 0.363, P 〈 0.001） and regimen-related distress domain （estimated 13 = 0.356, P 〈 0.001）. The correlation between SAS and DDS was positive （estimated 13 = 0.039, P = 0.027）. In conclusion, the results showed the im- portance of psychological aspects in Chinese individuals with type 1 diabetes. Screening and treatment of psycho- logical aspects may result in better adherence and increased quality of life for patients with diabetes.
基金supported by grant 81170747 from the National Natural Sciences Foundation of Chinagrant H201106 from Health Promotion Foundation of Jiangsu Provincegrant from the Office of Human Resources and Social Security of Jiangsu Province (Peak of the Six Personnel in Jiangsu Province) to Hongwen Zhou
文摘Multiple endocrine neoplasia type 2A （MEN2A）, a subtype of MEN2, is characterized by medullary thyroid cancer, pheochromocytoma, and primary hyperparathyroidism. A Han Chinese pedigree with MEN2A was investigated following confirmation of the proband＇s diagnosis by pathological findings and DNA/biochemical screening. DNA samples from 4 other family members were collected and exon 5, 8, 10, 11, 13, 16 and 18 of the RET proto-oncogene were sequenced and then analyzed. A missense mutation of TGG （Trp） to TGC （Cys） at codon 634 （the classic MEN2A mutation） in exon 11 of the RET gene was detected in 3 family members, including the proband. Sequencing data were compared with the human gene mutation database. Elevated serum calcitonin level was detected initially; medullary thyroid carcinoma was revealed in the 3 cases and adrenal pheochromocytoma was also found in the proband. Elective operations were successfully performed on the adrenal and thyroid glands because of pheochromocytoma and medullary thyroid carcinoma. Our case study confirms that integrated DNA-based/biochemical screening is crucial for early diagnosis of MEN2A and is helpful in the screening of their relatives. In addition, DNA-based screening may occasionally uncover a previously unknown RET sequence.
基金Supported by Study of Water Consumption Coefficient in the Irrigation Area of the Yellow River Basin in Qinghai Province(QX2012-019)
文摘The low degree of development and utilization as well as the contradiction between supply and demand of water resources in Huangshui River basin are the main restricting factors of the local agricultural development. The study on the simulation of irrigation water loss based on the VSMB model has very important significance to strengthening regional water management and improving water resource utilization efficiency. Five groundwater wells were set up to carry out the farmland irrigation water infiltration and the experimental study on groundwater dynamic effect. Two soil moisture monitoring sites were set up in two typical plots of Daxia and Guanting irrigation area at the same time and TDR300 was used to monitor four kinds of deep soil moisture( 10 cm,30 cm,50 cm and 70 cm). On this basis,the VSMB model was used to study the irrigation water loss in the irrigation area of Yellow River valley of Qinghai Province,including soil moisture content,the actual evapotranspiration,infiltration,runoff,groundwater buried depth and so on. The results showed that the water consumption caused by soil evaporation and crop transpiration accounted for 46. 4% and 24. 1% of the total precipitation plus irrigation,respectively,and the leakage accounted for 30. 3% and 60. 6% of the total precipitation plus irrigation,respectively,from March 1,2013 to April 30,and from August 1 to September 30. The actual evaporation of the GT- TR1 and GT- TR2 sites in the whole year of 2013 was 632. 6 mm and 646. 9 mm,respectively,and the leakage accounted for 2. 6% and 1. 2% of the total precipitation plus irrigation,respectively. RMSE of the simulation results of the groundwater depth in Daxia irrigation area during the two periods was 92. 3 mm and 27. 7 mm,respectively. And RMSE of the simulation results of the water content of soil profile in the two monitoring sites of Guanting irrigation area was 2. 04% and 5. 81%,respectively,indicating that the simulation results were reliable.
文摘<div style="text-align:justify;"> A new planar waveguide laser was demonstrated. The output energy of 400 mW was achieved by a single waveguide laser;the slope efficiency was 61%. The single waveguide laser can expand to waveguide laser group and waveguide laser array to produce higher energy. </div>
基金supported by the National Natural Science Foundation of China(81270897,81300668,81370939,81400813,81400808,81530026,81370922)the Jiangsu Provincial Special Program of Medical Science(BL2012026)the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions support this study
文摘Type 1 diabetes mellitus is heterogeneous in many facets. The patients suffered from type 1 diabetes present several levels of islet function as well as variable number and type of islet-specific autoantibodies. This study was to investigate prevalence and heterogeneity of the islet autoantibodies and clinical phenotypes of type 1 diabetes mellitus; and also discussed the process of islet failure and its risk factors in Chinese type 1 diabetic patients. A total of 1,291 type 1 diabetic patients were enrolled in this study. Demographic information was collected. Laboratory tests including mixed-meal tolerance test, human leukocyte antigen alleles, hemoglobin A1 c, lipids, thyroid function and islet autoantibodies were conducted. The frequency of islet-specific autoantibody in newly diagnosed T1 DM patients(duration shorter than half year) was 73% in East China. According to binary logistic regressions, autoantibody positivity, longer duration and lower Body Mass Index were the risk factors of islet failure. As the disease developed, autoantibodies against glutamic acid decarboxylase declined as well as the other two autoantibodies against zinc transporter 8 and islet antigen 2. The decrease of autoantibodies was positively correlated with aggressive beta cell destruction. Autoantibodies can facilitate the identification of classic T1 DM from other subtypes and predict the progression of islet failure. As there were obvious heterogeneity in autoantibodies and clinical manifestation in different phenotypes of the disease, we should take more factors into consideration when identifying type 1 diabetes mellitus.
