A homogeneous visible light photoredox TEMPO-mediated selective oxidation of primary alcohols to the corresponding carbonyl compounds was developed using molecular oxygen from air as the terminal oxidant.Ru(bpy)_(3)(P...A homogeneous visible light photoredox TEMPO-mediated selective oxidation of primary alcohols to the corresponding carbonyl compounds was developed using molecular oxygen from air as the terminal oxidant.Ru(bpy)_(3)(PF_(6))_(2)(bpy:bipyridyl)and Ir(dtb-bpy)(ppy)_(2)(PF_(6))(dtb-bpy:4,4'-di-tert-butyl-2,2'-bipyridyl;ppy:2-phenylpyridine)were used as the sensitizers.展开更多
Genome-scale metabolomics analysis is increasingly used for pathway and function discovery in the post-genomics era.The great potential offered by developed mass spectrometry(MS)-based technologies has been hindered,s...Genome-scale metabolomics analysis is increasingly used for pathway and function discovery in the post-genomics era.The great potential offered by developed mass spectrometry(MS)-based technologies has been hindered,since only a small portion of detected metabolites were identifiable so far.To address the critical issue of low identification coverage in metabolomics,we adopted a deep metabolomics analysis strategy by integrating advanced algorithms and expanded reference databases.The experimental reference spectra and in silico reference spectra were adopted to facilitate the structural annotation.To further characterize the structure of metabolites,two approaches were incorporated into our strategy,i.e.,structural motif search combined with neutral loss scanning and metabolite association network.Untargeted metabolomics analysis was performed on 150 rice cultivars using ultra-performance liquid chromatography coupled with quadrupoleOrbitrap MS.Consequently,a total of 1939 out of 4491 metabolite features in the MS/MS spectral tag(MS2T)library were annotated,representing an extension of annotation coverage by an order of magnitude in rice.The differential accumulation patterns of flavonoids between indica and japonica cultivars were revealed,especially O-sulfated flavonoids.A series of closely-related flavonolignans were characterized,adding further evidence for the crucial role of tricin-oligolignols in lignification.Our study provides an important protocol for exploring phytochemical diversity in other plant species.展开更多
基金Financial support is provided by National Natural Science Foundation of China(No.21102097)the Specialized Research Fund for the Doctoral Program of Higher Education(No.20103201120006)Beijing National Laboratory for Molecular Sciences(BNLMS).
文摘A homogeneous visible light photoredox TEMPO-mediated selective oxidation of primary alcohols to the corresponding carbonyl compounds was developed using molecular oxygen from air as the terminal oxidant.Ru(bpy)_(3)(PF_(6))_(2)(bpy:bipyridyl)and Ir(dtb-bpy)(ppy)_(2)(PF_(6))(dtb-bpy:4,4'-di-tert-butyl-2,2'-bipyridyl;ppy:2-phenylpyridine)were used as the sensitizers.
基金supported by grants from the Strategic Priority Research Program of Chinese Academy of Sciences(Grant No.XDA24010400)the National Key R&D Program of China(Grant Nos.2018YFA0900700 and 2019YFA0904601)+1 种基金the Major Project of Jiangsu Province of China for Significant New Varieties Development(Grant No.PZCZ201702)the National Natural Science Foundation of China(Grant Nos.31900470,31701137,and 31972881)。
文摘Genome-scale metabolomics analysis is increasingly used for pathway and function discovery in the post-genomics era.The great potential offered by developed mass spectrometry(MS)-based technologies has been hindered,since only a small portion of detected metabolites were identifiable so far.To address the critical issue of low identification coverage in metabolomics,we adopted a deep metabolomics analysis strategy by integrating advanced algorithms and expanded reference databases.The experimental reference spectra and in silico reference spectra were adopted to facilitate the structural annotation.To further characterize the structure of metabolites,two approaches were incorporated into our strategy,i.e.,structural motif search combined with neutral loss scanning and metabolite association network.Untargeted metabolomics analysis was performed on 150 rice cultivars using ultra-performance liquid chromatography coupled with quadrupoleOrbitrap MS.Consequently,a total of 1939 out of 4491 metabolite features in the MS/MS spectral tag(MS2T)library were annotated,representing an extension of annotation coverage by an order of magnitude in rice.The differential accumulation patterns of flavonoids between indica and japonica cultivars were revealed,especially O-sulfated flavonoids.A series of closely-related flavonolignans were characterized,adding further evidence for the crucial role of tricin-oligolignols in lignification.Our study provides an important protocol for exploring phytochemical diversity in other plant species.