This work proposed a label-flee, cost-effective and fairly sensitive electrogenerated chemiluminescence （ECL） strategy for the specific detection of lysozyme based on the hemin/G-quadruplex hybrid. Gold nanoparticle...This work proposed a label-flee, cost-effective and fairly sensitive electrogenerated chemiluminescence （ECL） strategy for the specific detection of lysozyme based on the hemin/G-quadruplex hybrid. Gold nanoparticles were spread onto the chitosan thin-film as substrate to adsorb thiolated captures： a hairpin-structured DNA, integrating dual head-tail connected functional domains： one aptamer sequence for lysozyme and the other for hemin （iron（II1） proto-porphyrin IX）. In the presence of the both, the hairpin conformation unfolded and transformed into the hemird G-quadruplex motif, which quenched the ECL emission of underlaid quantum dots significantly via the consump- tion of dissolved oxygen as endogenous coreactant. This construction enabled a wide linear response to lysozyme, ranging from 20 μg.mL-1 to 5μg. mL-1, with a detection limit as low as 4.95 pgo mL-1 （e.g. 9.4 pmol·L^-1）, dem- onstrating the prospective utilization of DNA technologies in bioanalysis.展开更多
文摘This work proposed a label-flee, cost-effective and fairly sensitive electrogenerated chemiluminescence （ECL） strategy for the specific detection of lysozyme based on the hemin/G-quadruplex hybrid. Gold nanoparticles were spread onto the chitosan thin-film as substrate to adsorb thiolated captures： a hairpin-structured DNA, integrating dual head-tail connected functional domains： one aptamer sequence for lysozyme and the other for hemin （iron（II1） proto-porphyrin IX）. In the presence of the both, the hairpin conformation unfolded and transformed into the hemird G-quadruplex motif, which quenched the ECL emission of underlaid quantum dots significantly via the consump- tion of dissolved oxygen as endogenous coreactant. This construction enabled a wide linear response to lysozyme, ranging from 20 μg.mL-1 to 5μg. mL-1, with a detection limit as low as 4.95 pgo mL-1 （e.g. 9.4 pmol·L^-1）, dem- onstrating the prospective utilization of DNA technologies in bioanalysis.
