Human neuroblastoma cells （SH-SY5Y） have similar structures and functions as neural cells and have been frequently used for cell culture studies of neural cell functions.Previous studies have revealed L-and N-type c...Human neuroblastoma cells （SH-SY5Y） have similar structures and functions as neural cells and have been frequently used for cell culture studies of neural cell functions.Previous studies have revealed L-and N-type calcium channels in SH-SY5Y cells.However,the distribution of the low-voltage activated calcium channel （namely called T-type calcium channel,including Cav3.1,Cav3.2,and Cav3.3） in SH-SY5Y cells remains poorly understood.The present study detected mRNA and protein expres-sion of the T-type calcium channel （Cav3.1,Cav3.2,and Cav3.3） in cultured SH-SY5Y cells using real-time polymerase chain reaction （PCR） and western blot analysis.Results revealed mRNA and protein expression from all three T-type calcium channel subtypes in SH-SY5Y cells.Moreover,Cav3.1 was the predominant T-type calcium channel subtype in SH-SY5Y cells.展开更多
The aim of this study was to observe the changes in monoamine and amino acid neurotransmitters in the telencephalon of rats at four functional states after exhaustive exercise and treatment with a Yang-invigorating co...The aim of this study was to observe the changes in monoamine and amino acid neurotransmitters in the telencephalon of rats at four functional states after exhaustive exercise and treatment with a Yang-invigorating compound recipe.The main components of this Chinese traditional medicine preparation included Radix Ginseng,Rhizoma Chuanxiong,Fructus Schisandrae,Cortex Cinnamomi,Cornu Cervi Pantotrichum,Radix Morindae Officinalis,and Gecko.This experiment showed that dopamine（DA）,5-hydroxyindole acetic acid（5-HIAA）,and γ-aminobutyric acid levels noticeably decreased,while DA/5-hydroxytryptamine（5-HT） increased.Furthermore,glutamate（Glu） and Glu/γ-aminobutyric acid significantly increased after 1 hour of exercise in rats in the exercise ＋ medication group.The 5-HT and 5-HT/5-HIAA levels noticeably decreased,and DA/5-HT and Glu levels showed a robust and significant increase immediately after exhaustive exercise.The 5-HT,5-HT/5-HIAA levels sharply decreased,while DA/5-HT,Glu and γ-aminobutyric acid levels increased at 12 hours after exhaustion recovery.The results prove that Chinese herbal formula for strengthening Yang can induce changes in neurotransmitters in the telencephalon of rats after exhaustive exercise during the recovery process,and further improve central nervous system function.展开更多
基金the National Natural Science Foundation of China,No.81100831the Medical Science Foundation of Guangdong Health Department,No.B2011303
文摘Human neuroblastoma cells （SH-SY5Y） have similar structures and functions as neural cells and have been frequently used for cell culture studies of neural cell functions.Previous studies have revealed L-and N-type calcium channels in SH-SY5Y cells.However,the distribution of the low-voltage activated calcium channel （namely called T-type calcium channel,including Cav3.1,Cav3.2,and Cav3.3） in SH-SY5Y cells remains poorly understood.The present study detected mRNA and protein expres-sion of the T-type calcium channel （Cav3.1,Cav3.2,and Cav3.3） in cultured SH-SY5Y cells using real-time polymerase chain reaction （PCR） and western blot analysis.Results revealed mRNA and protein expression from all three T-type calcium channel subtypes in SH-SY5Y cells.Moreover,Cav3.1 was the predominant T-type calcium channel subtype in SH-SY5Y cells.
基金Science and Technology Project of Shandong Province, No. 032050117
文摘The aim of this study was to observe the changes in monoamine and amino acid neurotransmitters in the telencephalon of rats at four functional states after exhaustive exercise and treatment with a Yang-invigorating compound recipe.The main components of this Chinese traditional medicine preparation included Radix Ginseng,Rhizoma Chuanxiong,Fructus Schisandrae,Cortex Cinnamomi,Cornu Cervi Pantotrichum,Radix Morindae Officinalis,and Gecko.This experiment showed that dopamine（DA）,5-hydroxyindole acetic acid（5-HIAA）,and γ-aminobutyric acid levels noticeably decreased,while DA/5-hydroxytryptamine（5-HT） increased.Furthermore,glutamate（Glu） and Glu/γ-aminobutyric acid significantly increased after 1 hour of exercise in rats in the exercise ＋ medication group.The 5-HT and 5-HT/5-HIAA levels noticeably decreased,and DA/5-HT and Glu levels showed a robust and significant increase immediately after exhaustive exercise.The 5-HT,5-HT/5-HIAA levels sharply decreased,while DA/5-HT,Glu and γ-aminobutyric acid levels increased at 12 hours after exhaustion recovery.The results prove that Chinese herbal formula for strengthening Yang can induce changes in neurotransmitters in the telencephalon of rats after exhaustive exercise during the recovery process,and further improve central nervous system function.
