DNA nanostructures have found widespread applications in areas including nanoelectronics and biomedicine.However,traditional DNA origami needs a long single-stranded virus DNA and hundreds of short DNA strands,which m...DNA nanostructures have found widespread applications in areas including nanoelectronics and biomedicine.However,traditional DNA origami needs a long single-stranded virus DNA and hundreds of short DNA strands,which make this method complicated and money-consuming.Here,we present a protocol for the assembly of DNA nanoribbons with only four oligonucleotides.DNA nanoribbons with different dimensions were successfully assembled with a 96-base scafford strand and three short staples.These biotinylated nanoribbons could also be decorated with streptavidins.This approach suggests that there exist great design spaces for the creation of simple nucleic acid nanostructures which could facilitate their application in plasmonic or drug delivery.展开更多
基金Starting Fund of Nanjing University of Posts and Telecommunications(No.214175).
文摘DNA nanostructures have found widespread applications in areas including nanoelectronics and biomedicine.However,traditional DNA origami needs a long single-stranded virus DNA and hundreds of short DNA strands,which make this method complicated and money-consuming.Here,we present a protocol for the assembly of DNA nanoribbons with only four oligonucleotides.DNA nanoribbons with different dimensions were successfully assembled with a 96-base scafford strand and three short staples.These biotinylated nanoribbons could also be decorated with streptavidins.This approach suggests that there exist great design spaces for the creation of simple nucleic acid nanostructures which could facilitate their application in plasmonic or drug delivery.
