Objective: To investigate the effect of MEK1 inhibitor PD98058 on Tec and ERK2 in HepG2 hepatoma cells. Methods: The expression of mRNA and protein of Tec and ERK2 in HepG2 cells was detected by immunocytochemistry as...Objective: To investigate the effect of MEK1 inhibitor PD98058 on Tec and ERK2 in HepG2 hepatoma cells. Methods: The expression of mRNA and protein of Tec and ERK2 in HepG2 cells was detected by immunocytochemistry as- say. After various concentration of PD98059 treatment, the expression of Tec and ERK2 mRNA in HepG2 cells was detected by RT-PCR and Western blotting. Results: Tec and ERK2 expressed highly in HepG2 cells. PD98059 obviously inhibited the expression of mRNA and protein of Tec and ERK2 in a dose-dependent manner, in which 40 μmol/L of PD98059 exhibited the strongest inhibiting effect. Conclusion: PD98058, as MEK1 inhibitor, can inhibit Tec, block the signal route of Ras/Raf/ERK and to impede the signal transduction in HepG2 cells. Tec may be the signal protein in the upper stream of Ras/Raf/ERK in hepatocarcinoma cells and is supposed to interact with the signal way of Ras/Raf/ERK.展开更多
We demonstrate a distributed optical fiber sensing system based on the Michelson interferometer of the phase sensitive optical time domain reflectometer （q0-OTDR） for acoustic measurement. Phase, amplitude, frequenc...We demonstrate a distributed optical fiber sensing system based on the Michelson interferometer of the phase sensitive optical time domain reflectometer （q0-OTDR） for acoustic measurement. Phase, amplitude, frequency response, and location information can be directly obtained at the same time by using the passive 3 ×3 coupler demodulation. We also set an experiment and successfully restore the acoustic information. Meanwhile, our system has preliminary realized acoustic-phase sensitivity around -150 dB （re rad/μPa） in the experiment.展开更多
基金Supported by grants from the National Natural Science Foundation of China (No. 30370341, 30570410)the Academic Foundation for Au-thors of National Excellent Doctoral Dissertation of China (No. 200261).
文摘Objective: To investigate the effect of MEK1 inhibitor PD98058 on Tec and ERK2 in HepG2 hepatoma cells. Methods: The expression of mRNA and protein of Tec and ERK2 in HepG2 cells was detected by immunocytochemistry as- say. After various concentration of PD98059 treatment, the expression of Tec and ERK2 mRNA in HepG2 cells was detected by RT-PCR and Western blotting. Results: Tec and ERK2 expressed highly in HepG2 cells. PD98059 obviously inhibited the expression of mRNA and protein of Tec and ERK2 in a dose-dependent manner, in which 40 μmol/L of PD98059 exhibited the strongest inhibiting effect. Conclusion: PD98058, as MEK1 inhibitor, can inhibit Tec, block the signal route of Ras/Raf/ERK and to impede the signal transduction in HepG2 cells. Tec may be the signal protein in the upper stream of Ras/Raf/ERK in hepatocarcinoma cells and is supposed to interact with the signal way of Ras/Raf/ERK.
基金This work was supported by the Shandong Natural Science Foundation （No. ZR2013FL028）, Science and Technology Development Project of Shandong Province （2014GGX 103019）, and Innovation and Achievement Transformation Projects of Shandong Province （2014ZZCX04206）.
文摘We demonstrate a distributed optical fiber sensing system based on the Michelson interferometer of the phase sensitive optical time domain reflectometer （q0-OTDR） for acoustic measurement. Phase, amplitude, frequency response, and location information can be directly obtained at the same time by using the passive 3 ×3 coupler demodulation. We also set an experiment and successfully restore the acoustic information. Meanwhile, our system has preliminary realized acoustic-phase sensitivity around -150 dB （re rad/μPa） in the experiment.
