Objective: To investigate the effects of steroidal alkaloids and sarcovagine D isolated from Sarcococca hookeriana var. digyna on cell-proliferation and secretion of inflammatory cytokines in TNF-a-induced human RA-FL...Objective: To investigate the effects of steroidal alkaloids and sarcovagine D isolated from Sarcococca hookeriana var. digyna on cell-proliferation and secretion of inflammatory cytokines in TNF-a-induced human RA-FLS MH7 A in vitro, and against complete Freund's adjuvant(CFA)-induced arthritis rats in vivo. Methods: CCK-8 assay was utilized to evaluate the anti-proliferation activity in vitro. In in-vivo study, rats were randomly divided into control group, model group(CFA), steroidal alkaloids(STA) groups(5.0, 2.5 and 1.25 g/kg BW),and sarcovagine D(SD) groups(50, 100 and 200 mg/kg BW), 10 rats for each group. To evaluate the anti-inflammation effect, the histology, biochemical parameters and expression of inflammatory cytokines were detected. Results: Steroidal alkaloids and sarcovagine D showed strong anti-proliferative activity during MH7 A cell culture proliferation and downregulated NO levels, and inflammatory cytokines(IL-1β, IL-6 and PGE_2) in vitro. The rats treated with CFA induced ankle inflammation and lymphocyte infiltration, upregulated IL-1β, TNF-α,IL-6, PGE_2, MDA and NO levels, and downregulated IL-10 and SOD. On the contrary,steroidal alkaloids ameliorated all the changes induced by CFA in rats, suggesting the antiinflammatory effect of steroidal alkaloids and sarcovagine D. Conclusions: The results suggest that Sarcococca hookeriana var. digyna has anti-inflammatory effect. Steroidal alkaloids and sarcovagine D has the potential to cure RA.展开更多
基金supported by the Open Fund Project of Hubei Provincial Key Laboratory of Oceurrence and Intervention of Rheumatic Diseases of China (Grant No. OIR 15005:OIR17003A)
文摘Objective: To investigate the effects of steroidal alkaloids and sarcovagine D isolated from Sarcococca hookeriana var. digyna on cell-proliferation and secretion of inflammatory cytokines in TNF-a-induced human RA-FLS MH7 A in vitro, and against complete Freund's adjuvant(CFA)-induced arthritis rats in vivo. Methods: CCK-8 assay was utilized to evaluate the anti-proliferation activity in vitro. In in-vivo study, rats were randomly divided into control group, model group(CFA), steroidal alkaloids(STA) groups(5.0, 2.5 and 1.25 g/kg BW),and sarcovagine D(SD) groups(50, 100 and 200 mg/kg BW), 10 rats for each group. To evaluate the anti-inflammation effect, the histology, biochemical parameters and expression of inflammatory cytokines were detected. Results: Steroidal alkaloids and sarcovagine D showed strong anti-proliferative activity during MH7 A cell culture proliferation and downregulated NO levels, and inflammatory cytokines(IL-1β, IL-6 and PGE_2) in vitro. The rats treated with CFA induced ankle inflammation and lymphocyte infiltration, upregulated IL-1β, TNF-α,IL-6, PGE_2, MDA and NO levels, and downregulated IL-10 and SOD. On the contrary,steroidal alkaloids ameliorated all the changes induced by CFA in rats, suggesting the antiinflammatory effect of steroidal alkaloids and sarcovagine D. Conclusions: The results suggest that Sarcococca hookeriana var. digyna has anti-inflammatory effect. Steroidal alkaloids and sarcovagine D has the potential to cure RA.
