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泛素特异性蛋白酶39在非小细胞肺癌中的表达及其对细胞增殖的影响 被引量:7
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作者 吉晓剑 杨秀林 +2 位作者 周厚荣 王文琴 许新梅 《中国现代医学杂志》 CAS 2018年第30期37-42,共6页
目的检测泛素特异性蛋白酶39(USP39)在非小细胞肺癌组织及细胞中的表达情况,并分析USP39的表达水平与85例非小细胞肺癌患者的临床病理特征的相关性,及USP39对非小细胞肺癌细胞系增殖、凋亡和周期的影响。方法利用免疫组织化学技术检测... 目的检测泛素特异性蛋白酶39(USP39)在非小细胞肺癌组织及细胞中的表达情况,并分析USP39的表达水平与85例非小细胞肺癌患者的临床病理特征的相关性,及USP39对非小细胞肺癌细胞系增殖、凋亡和周期的影响。方法利用免疫组织化学技术检测非小细胞肺癌石蜡组织标本中USP39蛋白的表达情况,分析非小细胞肺癌组织及癌旁组织中USP39的表达差异,利用Spearman秩相关分析USP39与非小细胞肺癌患者临床病理特征的相关性,利用实时荧光定量聚合酶链反应(q RT-PCR)检测非小细胞肺癌细胞系及支气管上皮样细胞系中USP39的表达,利用CCK-8实验检测USP39对非小细胞肺癌细胞增殖的影响,利用流式细胞术检测USP39对非小细胞肺癌细胞凋亡和周期的影响。结果 USP39蛋白在非小细胞肺癌组织中的阳性率高于癌旁组织(P <0.05);USP39蛋白的表达水平与非小细胞肺癌患者TNM分期呈正相关(P <0.05),而与其他临床病理特征无相关性(P>0.05);非小细胞肺癌细胞系中USP39的表达水平高于支气管上皮样细胞(P <0.05);转染USP39 siRNA的非小细胞肺癌细胞增殖能力低于转染USP39 NC者(P <0.05);转染USP39 siRNA的非小细胞肺癌细胞早期凋亡细胞比例高于转染USP39 NC者(P <0.05),细胞周期被阻滞于G1期。结论 USP39在非小细胞肺癌中表达升高,与患者TNM分期密切相关,可促进非小细胞肺癌的增殖。 展开更多
关键词 泛素特异性蛋白酶39 非小细胞肺癌 增殖 凋亡 周期
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Photon number resolvability of multi-pixel superconducting nanowire single photon detectors using a single flux quantum circuit
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作者 hou-rong zhou Kun-Jie Cheng +5 位作者 Jie Ren Li-Xing You Li-Liang Ying Xiao-Yan Yang Hao Li Zhen Wang 《Chinese Physics B》 SCIE EI CAS CSCD 2022年第5期636-640,共5页
Superconducting nanowire single-photon detectors(SNSPDs) are typical switching devices capable of detecting single photons with almost 100% detection efficiency. However, they cannot determine the exact number of inci... Superconducting nanowire single-photon detectors(SNSPDs) are typical switching devices capable of detecting single photons with almost 100% detection efficiency. However, they cannot determine the exact number of incident photons during a detection event. Multi-pixel SNSPDs employing multiple read-out channels can provide photon number resolvability(PNR), but they require increased cooling power and costly multi-channel electronic systems. In this work, a single-flux quantum(SFQ) circuit is employed, and PNR based on multi-pixel SNSPDs is successfully demonstrated. A multi-input magnetically coupled DC/SFQ converter(MMD2 Q) circuit with a mutual inductance M is used to combine and record signals from a multi-pixel SNSPD device. The designed circuit is capable of discriminating the amplitude of the combined signals in accuracy of Φ_(0)/M with Φ_(0) being a single magnetic flux quantum. By employing the MMD2 Q circuit,the discrimination of up to 40 photons can be simulated. A 4-parallel-input MMD2 Q circuit is fabricated, and a PNR of3 is successfully demonstrated for an SNSPD array with one channel reserved for the functional verification. The results confirm that an MMD2 Q circuit is an effective tool for implementing PNR with multi-pixel SNSPDs. 展开更多
关键词 superconducting nanowire single-flux quantum circuit photon number resolution
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