目前,大多数无害化处置运输车辆使用全球定位系统(global positioning system,GPS)来确定病死猪的消毒站位置和运输路线。然而,当车辆在偏远山区运行时,定位信息传递不连续和不完整。为了解决这个问题,设计了一套病死猪无害化处理监控系...目前,大多数无害化处置运输车辆使用全球定位系统(global positioning system,GPS)来确定病死猪的消毒站位置和运输路线。然而,当车辆在偏远山区运行时,定位信息传递不连续和不完整。为了解决这个问题,设计了一套病死猪无害化处理监控系统,系统结合北斗导航系统、全球定位系统、无线射频识别技术(radio frequency identification,RFID)、通用分组无线服务技术和地理信息系统等技术。利用北斗/GPS 双模用户机双模定位和双向通信的功能,一方面,它可以解决单一定位时出现的定位信息不稳定的问题;另一方面,当BDS导航定位系统数据似乎显示出偏差,将 WGS-84坐标系的GPS定位数据转换为BDS定位系统的CGCS2000坐标系,以提高运输车辆位置信息采集精度的目的。利用GAMIT软件计算观测点的三维坐标,并对组合导航和单导航的定位精度进行分析。采用Floyd算法对运输路线进行优化,寻找最短的运输距离。利用北斗卫星的信息通信功能代替全球移动通信系统(global system for mobile communications,GSM),短消息业务,实现无害化运输车辆与控制中心之间的信息远程传输。控制中心通过对接收到的数据进行分析,得到病死猪收运车的运输路线和定点消毒监测信息。系统试验表明:采集的数据能够准确及时地传输到控制中心,北斗/GPS 双模接收机定位精度比北斗卫星定位精度高 55.13%,比GPS卫星定位精度高 52.71%。北斗通信的网络丢包率为0.26%,消毒点的车辆定点识别误差率 0.97%,结果表明,满足病死猪无害化处理监控管理的要求,系统运行可靠、稳定。该系统的构建与应用,为其他病死动物无害化处理的综合管理与监控提供参考。展开更多
Suzhong swine is a hybrid breed derived from Taihu sows and Landraee boars. To identify Suzhong swine individuals and trace the source of pork products, single nucleotide polymorphisms (SNPs) identification of Suzho...Suzhong swine is a hybrid breed derived from Taihu sows and Landraee boars. To identify Suzhong swine individuals and trace the source of pork products, single nucleotide polymorphisms (SNPs) identification of Suzhong swine individuals was studied. A total of 29 pairs of primers were designed and sev- en pairs of primers were used for identification of Suzhong swine individuals. The products amplified by seven pairs of primers could be directly sequenced, with clean sequencing map background and no ambiguity in sequence read. Totally 52 SNPs loci were amplified by seven pairs of primers, and 41 SNPs loci were reserved for identification of Suzhong swine individuals through correlation analysis and heterezygosity filtration ( H ≥0.1 ). Meantime, the digital barcodes for SNP identification of 96 individuals of Suzhong swine derived from seven boars and 12 sows were developed, which well distinguished 96 individuals of Suzhong swine. Theoretically, 41SNPs amplified by seven pairs of primers could be used for identification of 5.0 × 10^6 pig individuals. Therefore, digital barcode devel- opment method for SNP identification of Suzbong swine individuals can be used for individual identification of Suzhong swine in scale pig farm and meat product traceability.展开更多
文摘目前,大多数无害化处置运输车辆使用全球定位系统(global positioning system,GPS)来确定病死猪的消毒站位置和运输路线。然而,当车辆在偏远山区运行时,定位信息传递不连续和不完整。为了解决这个问题,设计了一套病死猪无害化处理监控系统,系统结合北斗导航系统、全球定位系统、无线射频识别技术(radio frequency identification,RFID)、通用分组无线服务技术和地理信息系统等技术。利用北斗/GPS 双模用户机双模定位和双向通信的功能,一方面,它可以解决单一定位时出现的定位信息不稳定的问题;另一方面,当BDS导航定位系统数据似乎显示出偏差,将 WGS-84坐标系的GPS定位数据转换为BDS定位系统的CGCS2000坐标系,以提高运输车辆位置信息采集精度的目的。利用GAMIT软件计算观测点的三维坐标,并对组合导航和单导航的定位精度进行分析。采用Floyd算法对运输路线进行优化,寻找最短的运输距离。利用北斗卫星的信息通信功能代替全球移动通信系统(global system for mobile communications,GSM),短消息业务,实现无害化运输车辆与控制中心之间的信息远程传输。控制中心通过对接收到的数据进行分析,得到病死猪收运车的运输路线和定点消毒监测信息。系统试验表明:采集的数据能够准确及时地传输到控制中心,北斗/GPS 双模接收机定位精度比北斗卫星定位精度高 55.13%,比GPS卫星定位精度高 52.71%。北斗通信的网络丢包率为0.26%,消毒点的车辆定点识别误差率 0.97%,结果表明,满足病死猪无害化处理监控管理的要求,系统运行可靠、稳定。该系统的构建与应用,为其他病死动物无害化处理的综合管理与监控提供参考。
基金Supported by Agricultural Science and Technology Innovation Project of Jiangsu Province[CX(16)1006]Fundamental Research Fund of Jiangsu Academy of Agricultural Sciences[ZX(15)3003]
文摘Suzhong swine is a hybrid breed derived from Taihu sows and Landraee boars. To identify Suzhong swine individuals and trace the source of pork products, single nucleotide polymorphisms (SNPs) identification of Suzhong swine individuals was studied. A total of 29 pairs of primers were designed and sev- en pairs of primers were used for identification of Suzhong swine individuals. The products amplified by seven pairs of primers could be directly sequenced, with clean sequencing map background and no ambiguity in sequence read. Totally 52 SNPs loci were amplified by seven pairs of primers, and 41 SNPs loci were reserved for identification of Suzhong swine individuals through correlation analysis and heterezygosity filtration ( H ≥0.1 ). Meantime, the digital barcodes for SNP identification of 96 individuals of Suzhong swine derived from seven boars and 12 sows were developed, which well distinguished 96 individuals of Suzhong swine. Theoretically, 41SNPs amplified by seven pairs of primers could be used for identification of 5.0 × 10^6 pig individuals. Therefore, digital barcode devel- opment method for SNP identification of Suzbong swine individuals can be used for individual identification of Suzhong swine in scale pig farm and meat product traceability.