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The effects of microfilament and microtubule inhibitors and periodic electrical impulses on phloem transport in pea seedling 被引量:1
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作者 Guo Yuhai hua baoguang +2 位作者 Yu Fengyi Leng Qiang Lou Chenghou 《Chinese Science Bulletin》 SCIE EI CAS 1998年第4期312-314,共3页
In phloem transport, whether protoplasmic activity participates in assisting sap flow in sieve element_companion cell complex has long been in debate. The present investigation assumed microfilament (MF) and microtubu... In phloem transport, whether protoplasmic activity participates in assisting sap flow in sieve element_companion cell complex has long been in debate. The present investigation assumed microfilament (MF) and microtubule (MT), the two constituents of the protoplasmic cytoskeleton, as motive force, and employed germinating pea seedling suspended in moist chamber as experimental material: the seed being the source; the elongating root, the sink. 14 C_labeled sucrose was added to the seed as indicator. The amount of sap transported from source to sink was measured by the increase in root elongation. The transport phloem was within the cylinder of the peeled root in the middle. The exposed cylinder was treated with MF inhibitor (cytochalasin B), or microtubule inhibitor (amiphos_methyl). Results showed that the sap influx into the elongating root, and the 14 C activity as well, was reduced by about one half in treatment with cytochalasin B, and much less by amiphos_methyl treatment. Similar effect was shown in electrical impulse treatment, which seems to disrupt the MF and MT configuration. 展开更多
关键词 PHLOEM transport ELECTRICAL impulse MICROFILAMENT MICROTUBULE pea seedling.
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