基于绿色建筑背景下的集装箱建筑的应用研究

集装箱建筑作为新兴建筑体系具有其独特性,以绿色建筑为背景,对集装箱建筑发展历程、集装箱建筑应用现状、集装箱建筑应用性质进行总结与深入探讨,同时对集装箱发展前景进行展望,促进集装箱建筑作为可持续性的绿色建筑形态的优化与发展,提高建筑舒适度的同时使人、建筑、自然更好的融合。

Neurochip Based on Light-addressable Potentiometric Sensor

<正>A novel neurochip based on light addressable potentiometric sensor (LAPS) is designed.Using its light addressable characteristic.The problems of the limitations of restricted discrete active sites of current neurochips,such as microelectrode array and field effect transistor array can be settled easily.Based on the theoretical analysis of the interface between cells and LAPS,spontaneously discharges of hippocampal neurons induced by Mg~ 2+)-free media treatment were recorded by LAPS.The results demonstrate that this kind of neurochip has potential to monitor electrophysiology of cultured cells in a non-invasive way.

DESIGN OF MICROPHYSIOMETER BASED ON MULTIPARAMETER CELL-BASED BIOSENSORS FOR QUICK DRUG ANALYSIS

Cellular metabolism arouses the changes of substance in extracellular physiological microenvironment,and the metabolic level reflects the physiological state of cells.This paper developed a novel microphysiometer automatic analysis instrument based on multiparameter cell-based biosensors for quick drug analysis.This study included the multiparameter cell-based biosensors,cell culture chamber,drug auto-injection detection and analysis.The analysis instrument was capable of real-time detection for the acidic product and other chemical parameters generated by the cellular metabolism in the micro-volume.Finally,the paper employs human breast cancer cell line MCF-7 and drug experiments to verify the performance of microphysiometer,and study effects of different drugs on cell metabolism.Further,the research explores drug analysis method of the multiparameter microphysiometer.The results showed that the cell-based microphysiometer system provides a utility platform for rapid,long-term and automatic cell physiological environment detection and drug analysis.

A Novel Structure of LAPS Array for Cell-Based Biosensor

The light-addressable potentiometric sensor (LAPS) is a semiconductor-based cellular biosensor with an electrolyte-insulator-semiconductor (EIS) structure.By depositing biocompatible layers on the sensing surface for cell culture, it can be used to detect bioelectrical parameters of cells.The characteristic curve for photocurrent versus applied bias voltage to the system shows a current-voltage curve (Ⅰ-Ⅴcurve).This technique can be used to detect the action potential changes towards different drugs based on the bias voltage dependence of an optical current,and provides a dynamic system by scanning light beam at the very cell on the sensor device.The LAPS overcomes the limitation of recording sites,but high spatial resolution and sensitivity are also paramount.This paper discussed a novel structure of LAPS array for extracellular monitoring to decrease potential noise level.Both characteristics of active recording array areas and cell culture conditions are measured.

自然能源相关的绿色技术的发展趋势——基于全国绿色建筑竞赛获奖方案的研究

在全国绿色建筑大赛中,许多获奖方案都运用了各类绿色技术来丰富优化建筑与环境的关系,本文在分析全国绿色建筑竞赛获奖方案中所应用的绿色技术的基础上,着重研究与自然能源相关的绿色技术的发展背景与历程,基于现状及未来的趋势进行研究并总结,旨在阐述绿色建筑中与自然能源相关的绿色技术的发展趋势,实现建筑的绿色可持续。

Understanding the demand predictability of bike share systems:A station-level analysis

Predicting demand for bike share systems(BSSs)is critical for both the management of an existing BSS and the planning for a new BSS.While researchers have mainly focused on improving prediction accuracy and analysing demand-influencing factors,there are few studies examining the inherent randomness of stations'observed demands and to what degree the demands at individual stations are predictable.Using Divvy bike-share one-year data from Chicago,USA,we measured demand entropy and quantified the station-level predictability.Additionally,to verify that these predictability measures could represent the performance of prediction models,we implemented two commonly used demand prediction models to compare the empirical prediction accuracy with the calculated entropy and predictability.Furthermore,we explored how city-and system-specific temporallyconstant features would impact entropy and predictability to inform estimating these measures when historical demand data are unavailable.Our results show that entropy and predictability of demands across stations are polarized as some stations exhibit high uncertainty(a low predictability of 0.65)and others have almost no check-out demand uncertainty(a high predictability of around 1.0).We also validated that the entropy and predictability are a priori model-free indicators for prediction error,given a sequence of bike usage demands.Lastly,we identified that key factors contributing to station-level entropy and predictability include per capita income,spatial eccentricity,and the number of parking lots near the station.Findings from this study provide more fundamental understanding of BSS demand prediction,which can help decision makers and system operators anticipate diverse stationlevel prediction errors from their prediction models both for existing stations and for new ones.

Long-term agricultural contamination shaped diversity response of sediment microbiome

The pollution caused by agricultural production poses a threat to the ecological integrity of river ecosystems,altering the structure and function of river ecosystems.Differences in microbial community structure provide useful information about the impact of agricultural pollution on the biological integrity of ecosystems,but generally convey little information regarding ecosystem functions.In this study,using Illumina MiSeq sequencing technology based on the 16 S rRNA gene,river sediment samples associated with four different types of agricultural pollution were comprehensively analyzed.The results show that the total organic carbon(TOC)content was highest at the YZS site(animal husbandry sewage)among the assayed sites,but the species richness and uniformity were lowest at this site,which may have been caused by the high nutrient source of the sewage.Furthermore,in the three YZS samples affected by the long-term discharge of aquaculture tail-water,the unique genus Dechloromonas and the genus Candidatus-Competitor were observed,which are strongly correlated with phosphorus conversion.The formation of network modules may correspond to the coexistence of functional bacteria accustomed to multiple niche combinations under different agricultural pollution conditions in river sediments.According to the PICRUSt functional prediction,the bacterial community in the agricultural polluted river sediment primarily harbored 46 subfunctions,exhibiting richness of functions.Overall,our results provide a more comprehensive understanding of the structure and ecological processes associated with the aggregation of bacterial communities,which is beneficial for the management of river environments.

A Totally(Δ+1)-colorable 1-planar Graph with Girth at Least Five

A graph is 1-planar if it can be drawn on the plane so that each edge is crossed by at most one other edge. In this paper, we prove that every 1-planar graph G with maximum degree △（G） 〉 12 and girth at least five is totally （△（G）＋1）-colorable.

An online service-oriented performance profiling tool for cloud computing systems

The growing scale and complexity of component interactions in cloud computing systems post great challenges for operators to understand the characteristics of system performance. Profiling has long been proved to be an effective approach to performance analysis; however, existing approaches confront new challenges that emerge in cloud computing systems. First, the efficiency of the profiling becomes of critical concern; second, service-oriented profiling should be considered to support separation-of-concerns performance analysis. To address the above issues, in this paper, we present P-Tracer, an online performance profiling tool specifically tailored for cloud computing systems. P-Tracer constructs a specific search engine that proactively processes performance logs and generates a particular index for fast queries; second, for each service, P-Tracer retrieves a statistical insight of performance characteristics from multi-dimensions and provides operators with a suite of web-based interfaces to query the critical information. We evaluate P- Tracer in the aspects of tracing overheads, data preprocessing scalability and querying efficiency. Three real-world case studies that happened in Alibaba cloud computing platform demonstrate that P-Tracer can help operators understand soft-ware behaviors and localize the primary causes of performance anomalies effectively and efficiently.

A photovoltage-based integrated sensor for extracellular redox potential measurement and acidification detection

In this study,photovoltage technique is applied in the development of a monolithically integrated sensor for redox(reduction–oxidation)potential and pH measurement.The sensor employs the electrolyte–insulator–semiconductor structure,with deposition of a layer of gold metal on partial surface of insulator silicon dioxide.Silicon dioxide and gold layer on a single chip form two distinct sensing sites,by sharing the same measuring system,the detection of redox potential and pH variation can be realized.In this work,the sensor characteristics is tested,and the sensitivity for redox potential and pH measurement is53.8 mV/log([Fe(II)]/[Fe(III)])and 44.3 mV/pH respectively.To demonstrate the validity of the sensor in extracellular detection,neonatal rat kidney cells are cultured on the sensor surface and then packaged in a flow chamber,thus the acidification rate of metabolites and the redox potential variation in extracellular microenvironment can be continuously monitored.Experimental results indicate increasing acidification and reducing potentials under physiological conditions.The synthesis parameters have potentials in detail revelation of cell metabolism.

On Total Colorings of Some Special 1-planar Graphs

A graph is 1-planar if it can be drawn on the plane so that each edge is crossed by at most one other edge. In this paper, we verify the total coloring conjecture for every 1-planar graph G if either △（G） ≥9 and g（G）≥ 4, or △（G） ≥ 7 and g（G）≥5, where △（G） is the maximum degree of G and g（G） is the girth of G.

Total Coloring of Planar Graphs without Chordal 7-cycles

A k-total-coloring of a graph G is a coloring of vertices and edges of G using k colors such that no two adjacent or incident elements receive the same color.In this paper,it is proved that if G is a planar graph with Δ（G） ≥ 7 and without chordal 7-cycles,then G has a（Δ（G） ＋ 1）-total-coloring.