Best time for progesterone supplementation in aid ovulation induction cycles by letrozole

Objective:To explore the best time for progesterone supplementation in AID ovulation induction cycles by Letrozole.Methods: The data analysed in this study were collected from 509 patients who were performed AID (Artificial Insemination by Donor) administrated letrozole (LE) between 2014.8-2015.7. All patients were randomly divided into 4 groups by the time of progesterone administrated, including experimental group and the control group. The experimental group was divided into group 1-72 h after ovulation, group 2-48 h after ovulation, group 3-24 h after ovulation and control group—without administrated LE. The gestation and live birth rate were evaluated by monitoring vaginal ultrasound and HCG blood value 14 d after AID.Results: The pregnancy rate with administrated progesterone added 72 h after ovulation was 31.9%, which was significantly higher than those of other groups, the same situation as groups added progesterone was significantly higher than the control group. However, there was no significant difference in the numbers of abortions among the four groups. The LBR of group 4 was significantly lower than that of group 1.Conclutions: Progesterone administrated 72 h after ovulation can promoted the gestation rate, but did not affect the rate of miscarrage .

A Blm–Recql5 partnership in replication stress response

Deficiencies in DNA damage response and repair not only can result in genome instability and cancer predisposition,but also can render the cancer cells intrinsically more vulnerable to certain types of DNA damage insults.Particularly,replication stress is both a hallmark of human cancers and a common instigator for genome instability and cell death.Here,we review our work based on the genetic knockout studies on Blm and Recql5,two members of the mammalian RecQ helicase family.These studies have uncovered a unique partnership between these two helicases in the implementation of proper mitigation strategies under different circum-stances to promote DNA replication and cell survival and suppress genome instability and cancer.In particular,current studies have revealed the presence of a novel Recql5/RECQL5-dependent mechanism for suppressing replication fork collapse in response to global replication fork stalling following exposure to camptothecin(CPT),a topoisomerase I inhibitor,and a potent inhibitor of DNA replication.The unique partnership between Blm and Recql5 in coping with the challenge imposed by replication stress is discussed.In addition,given that irinotecan and topotecan,two CPT derivatives,are currently used in clinic for treating human cancer patients with very promising results,the potential implication of the new findings from these studies in anticancer treatments is also discussed.