Objective:To study the relationship of different respiratory virus infection with pediatric asthma attack as well as cytokine and lymphocyte subset levels.Methods: A total of 85 children who were diagnosed with bronch...Objective:To study the relationship of different respiratory virus infection with pediatric asthma attack as well as cytokine and lymphocyte subset levels.Methods: A total of 85 children who were diagnosed with bronchial asthma in our hospital between May 2013 and March 2016 were selected as asthma group and further divided into asthma-RSV group, asthma-AV group, asthma-PIV group, asthma-IFV group and pure asthma group according to the condition of respiratory virus infection, and 70 healthy children who received physical examination in our hospital during the same period were selected as the control group. Spirometer was used to determine airway function parameters, enzyme-linked immunosorbent assay kits were used to determine serum cytokine contents, and flow cytometry was used to determine peripheral blood lymphocyte subset contents.Results: FEV1/FVC, FEF25, FEF50 and FEF75 levels, serum IL-2, IFN-γ and TGF-β1 contents as well as peripheral blood Th1 and Treg cell contents of asthma groups were significantly lower than those of control group while serum IL-4, IL-5 and IL-17 contents as well as peripheral blood Th2 and Th17 cell contents were significantly higher than those of control group;FEV1/FVC, FEF25, FEF50 and FEF75 levels, serum IL-2, IFN-γ and TGF-β1 contents as well as peripheral blood Th1 and Treg cell contents of asthma-RSV group and asthma-IFV group were significantly lower than those of pure asthma group while serum IL-4, IL-5 and IL-17 contents as well as peripheral blood Th2 and Th17 cell contents were significantly higher than those of pure asthma group;these indexes of asthma-AV group and asthma-PIV group were not significantly different from those of pure asthma group.Conclusion: RSV and IFV infection can affect the airway function and the balance of CD4+T cell subsets to promote the development of asthma.展开更多
Objective:To study the effect of pediatric asthma complicated by respiratory virus infection on airway remodeling and inflammation.Methods:A total of 41 children with asthma complicated by respiratory virus infection ...Objective:To study the effect of pediatric asthma complicated by respiratory virus infection on airway remodeling and inflammation.Methods:A total of 41 children with asthma complicated by respiratory virus infection who were treated in our hospital between May 2012 and March 2016 were collected as observation group, and 50 children with asthma alone who were treated in our hospital during the same period were selected as control group. High-resolution CT was used to determine the right upper lobe apical segment (RB1) and the left lower lobe posterior basal segment (LB10) airway remodeling parameters of two groups of patients, and serum airway remodeling index and inflammatory factor contents were detected. Results: LA, WA and TA levels of RB1 and LB10 of observation group were significantly lower than those of control group, serum airway remodeling indexes TGF-β1, Smad3, PⅠNP and PⅢNP contents were higher than those of control group, serum inflammation indexes IL-4, IL-5 and TNF-α contents were higher than those of control group.Conclusion:Complication of respiratory virus infection can aggravate the airway remodeling and systemic inflammation in children with asthma.展开更多
Accumulated evidence suggested that gut microbial dysbiosis interplayed with progressive chronic kidney disease(CKD).However,no available therapy is effective in suppressing progressive CKD.Here,using microbiomics in ...Accumulated evidence suggested that gut microbial dysbiosis interplayed with progressive chronic kidney disease(CKD).However,no available therapy is effective in suppressing progressive CKD.Here,using microbiomics in 480 participants including healthy controls and patients with stage 1–5 CKD,we identified an elongation taxonomic chain Bacilli-Lactobacillales-Lactobacillaceae-Lactobacillus-Lactobacillus johnsonii correlated with patients with CKD progression,whose abundance strongly correlated with clinical kidney markers.L.johnsonii abundance reduced with progressive CKD in rats with adenine-induced CKD.L.johnsonii supplementation ameliorated kidney lesion.Serum indole-3-aldehyde(IAld),whose level strongly negatively correlated with creatinine level in CKD rats,decreased in serum of rats induced using unilateral ureteral obstruction(UUO)and 5/6 nephrectomy(NX)as well as late CKD patients.Treatment with IAld dampened kidney lesion through suppressing aryl hydrocarbon receptor(AHR)signal in rats with CKD or UUO,and in cultured 1-hydroxypyrene-induced HK-2 cells.Renoprotective effect of IAld was partially diminished in AHR deficiency mice and HK-2 cells.Our further data showed that treatment with L.johnsonii attenuated kidney lesion by suppressing AHR signal via increasing serum IAld level.Taken together,targeting L.johnsonii might reverse patients with CKD.This study provides a deeper understanding of how microbial-produced tryptophan metabolism affects host disease and discovers potential pathways for prophylactic and therapeutic treatments for CKD patients.展开更多
文摘Objective:To study the relationship of different respiratory virus infection with pediatric asthma attack as well as cytokine and lymphocyte subset levels.Methods: A total of 85 children who were diagnosed with bronchial asthma in our hospital between May 2013 and March 2016 were selected as asthma group and further divided into asthma-RSV group, asthma-AV group, asthma-PIV group, asthma-IFV group and pure asthma group according to the condition of respiratory virus infection, and 70 healthy children who received physical examination in our hospital during the same period were selected as the control group. Spirometer was used to determine airway function parameters, enzyme-linked immunosorbent assay kits were used to determine serum cytokine contents, and flow cytometry was used to determine peripheral blood lymphocyte subset contents.Results: FEV1/FVC, FEF25, FEF50 and FEF75 levels, serum IL-2, IFN-γ and TGF-β1 contents as well as peripheral blood Th1 and Treg cell contents of asthma groups were significantly lower than those of control group while serum IL-4, IL-5 and IL-17 contents as well as peripheral blood Th2 and Th17 cell contents were significantly higher than those of control group;FEV1/FVC, FEF25, FEF50 and FEF75 levels, serum IL-2, IFN-γ and TGF-β1 contents as well as peripheral blood Th1 and Treg cell contents of asthma-RSV group and asthma-IFV group were significantly lower than those of pure asthma group while serum IL-4, IL-5 and IL-17 contents as well as peripheral blood Th2 and Th17 cell contents were significantly higher than those of pure asthma group;these indexes of asthma-AV group and asthma-PIV group were not significantly different from those of pure asthma group.Conclusion: RSV and IFV infection can affect the airway function and the balance of CD4+T cell subsets to promote the development of asthma.
文摘Objective:To study the effect of pediatric asthma complicated by respiratory virus infection on airway remodeling and inflammation.Methods:A total of 41 children with asthma complicated by respiratory virus infection who were treated in our hospital between May 2012 and March 2016 were collected as observation group, and 50 children with asthma alone who were treated in our hospital during the same period were selected as control group. High-resolution CT was used to determine the right upper lobe apical segment (RB1) and the left lower lobe posterior basal segment (LB10) airway remodeling parameters of two groups of patients, and serum airway remodeling index and inflammatory factor contents were detected. Results: LA, WA and TA levels of RB1 and LB10 of observation group were significantly lower than those of control group, serum airway remodeling indexes TGF-β1, Smad3, PⅠNP and PⅢNP contents were higher than those of control group, serum inflammation indexes IL-4, IL-5 and TNF-α contents were higher than those of control group.Conclusion:Complication of respiratory virus infection can aggravate the airway remodeling and systemic inflammation in children with asthma.
基金supported by National Natural Science Foundation of China(Nos.82074002,82274079,82274192,32100631)Shaanxi Key Science and Technology Plan Project(No.2023-ZDLSF-26)+3 种基金Macao Young Scholars Program(No.AM2023024)China Postdoctoral Science Foundation(No.2023T160061)Capital’s Funds for Health Improvement and Research(No.2024-4-40215)Natural Science Foundation of Zhejiang Province(No.LZ22H280001).
文摘Accumulated evidence suggested that gut microbial dysbiosis interplayed with progressive chronic kidney disease(CKD).However,no available therapy is effective in suppressing progressive CKD.Here,using microbiomics in 480 participants including healthy controls and patients with stage 1–5 CKD,we identified an elongation taxonomic chain Bacilli-Lactobacillales-Lactobacillaceae-Lactobacillus-Lactobacillus johnsonii correlated with patients with CKD progression,whose abundance strongly correlated with clinical kidney markers.L.johnsonii abundance reduced with progressive CKD in rats with adenine-induced CKD.L.johnsonii supplementation ameliorated kidney lesion.Serum indole-3-aldehyde(IAld),whose level strongly negatively correlated with creatinine level in CKD rats,decreased in serum of rats induced using unilateral ureteral obstruction(UUO)and 5/6 nephrectomy(NX)as well as late CKD patients.Treatment with IAld dampened kidney lesion through suppressing aryl hydrocarbon receptor(AHR)signal in rats with CKD or UUO,and in cultured 1-hydroxypyrene-induced HK-2 cells.Renoprotective effect of IAld was partially diminished in AHR deficiency mice and HK-2 cells.Our further data showed that treatment with L.johnsonii attenuated kidney lesion by suppressing AHR signal via increasing serum IAld level.Taken together,targeting L.johnsonii might reverse patients with CKD.This study provides a deeper understanding of how microbial-produced tryptophan metabolism affects host disease and discovers potential pathways for prophylactic and therapeutic treatments for CKD patients.
