Effect of HK3 on immune invasion, proliferation and invasion of colon cancer cells

Objective:Investigate the effects of Hexokinase-3 on the proliferation,cell cycle,migration and immunologic invasion of RKO and HCT116.Methods:The expression levels of HK3 in tumor and normal colon samples were analyzed by bioinformatics.RKO and HCT116 were transfected with the control group(si-NC)and interference group HK3(si-HK3#1,si-HK3#2).CCK-8,Flow cytometry,wound healing and Transwell were used to examine the effects of interference with HK3 on the proliferation,cell cycle and migration of RKO and HCT116 cells.The expression levels of vimentin and E-cadherin,which are related to epithelial-mesenchymal transition(EMT),were detected by Western blot.Results:HK3 is associated with infiltration of multiple immune cells and co-expression with multiple immune checkpoints in colorectal cancer.Compared with the Control group(si-NC),the protein expression level of HK3 in interference group(si-HK3#1,si-HK3#2)was significantly decreased(P<0.05);the proliferation and migration of RKO and HCT116 cells were significantly inhibited(P<0.05);the cell cycle of RKO and HCT116 cells was arrested in S phase(P<0.05)and the expression of E-cadherin protein was increased,but the expression of vimentin protein was inhibited(P<0.05).Conclusion:HK3 affects the level of immunologic invasion of colon cancer.Interference with HK3 inhibits the proliferation,cell cycle and migration of colon cancer cells by inhibiting EMT behavior.

Growth of solidified shell in bloom continuous casting mold of hypo-peritectic steel based on a FeS tracer method

Solidification behavior in the mold region plays an important role in production efficiency and steel quality.To investigate shell growth within a mold,the sulfur prints of the entire shell thickness profile the meniscus to 100 mm below the mold were obtainedadding FeS tracer into molten steel during bloom continuous casting of hypo-peritectic steel.The law of shell thickness evolution along mold height and circumference was analyzed.The results show that there are three weak regions of solidification,which are in the mold upper part,in the mold lower part,and just below mold exit,possibly resulting periodic fluctuation of air gap between the shell and the mold,the impingement of melt jets on the solidification front,and the decreasing cooling intensity,respectively.Initial solidification point along casting direction appears at approximately 35 mm below the meniscus.Overall,the solidified shell thickness in the inner side of the mold is a little larger than that in the outer side,and the former and the latter reach 25.5 and 24.3 mm at the mold exit,respectively.The non-uniform shell growth in the inner side of the bloom is provided,while shell thicknesses in the narrow face and the outer side follow relatively regular growth.Out of the mold,the thinnest shells on the transverse section exist in the regions of 60&ndash;90 mm and 40&ndash;70 mm the corners of the inner and outer sides,respectively,i.e.,the of-corners.