Polypropylene(PP)scaffolds are the most commonly used biomedical scaffolds despite their disadvan-tages,which include problems with adhesion,infection,and inflammatory responses.Here,we report on the successful develo...Polypropylene(PP)scaffolds are the most commonly used biomedical scaffolds despite their disadvan-tages,which include problems with adhesion,infection,and inflammatory responses.Here,we report on the successful development of a facile one-step method to fabricate a series of novel triclosan poly-dopamine polypropylene(TPP)composite scaffolds and thereby effectively improve the biocompatibility and long-term antibacterial properties of PP scaffolds.The antibacterial triclosan can effectively interact with dopamine during biocompatible polydopamine formation on the PP scaffold by one-step green fab-rication.Thanks to the sustained release of triclosan from the biocompatible polydopamine coating,a 5mm×5mm sample of TPP-coated scaffold made with a triclosan concentration of 8 mg-mL^(-1)(referredto herein as TPP-8)exhibited a continuous antibacterial effect against both Escherichia coli(E.coli)and Staphylococcus aureus(S.aureus)for more than 15d,at maximum antibacterial volumes of 2 and 5mL,respectively.Our study establishes a new direction for facile long-term antibacterial studies for medical applications.展开更多
Background A significant difference in the anastomotic leakage(AL)rate has been observed between patients with locally advanced rectal cancer who have undergone preoperative chemotherapy and those undergoing preoperat...Background A significant difference in the anastomotic leakage(AL)rate has been observed between patients with locally advanced rectal cancer who have undergone preoperative chemotherapy and those undergoing preoperative chemoradiotherapy.This study aimed to quantitatively analyse collagen structural changes caused by preoperative chemoradiotherapy and illuminate the relationship between collagen changes and AL.Methods Anastomotic distal and proximal“doughnut”specimens from the Sixth Affiliated Hospital of Sun Yat-sen University(Guangzhou,China)were quantitatively assessed for collagen structural changes between patients with and without preoperative radiotherapy using multiphoton imaging.Then,patients treated with preoperative chemoradiotherapy were used as a training cohort to construct an AL–SVM classifier by the Mann–Whitney U test and support vector machine(SVM).An independent test cohort from the Fujian Province Cancer Hospital(Fuzhou,China)was used to validate the AL–SVM classifier.Results A total of 207 patients were included from the Sixth Affiliated Hospital of Sun Yat-sen University.The AL rate in the preoperative chemoradiotherapy group(n=107)was significantly higher than that in the preoperative chemotherapy group(n=100)(21.5%vs 7.0%,P=0.003).A fully quantitative analysis showed notable morphological and spatial distribution feature changes in collagen in the preoperative chemoradiotherapy group.Then,the patients who received preoperative chemoradiotherapy were used as a training cohort to construct the AL–SVM classifier based on five collagen features and the tumor distance from the anus.The AL–SVM classifier showed satisfactory discrimination and calibration with areas under the curve of 0.907 and 0.856 in the training and test cohorts,respectively.Conclusions The collagen structure may be notably altered by preoperative radiotherapy.The AL–SVM classifier was useful for the individualized prediction of AL in rectal cancer patients undergoing preoperative chemoradiotherapy.展开更多
基金support from the project of the Science and Technology Program of Guangzhou(201704020059 and 201803010074).
文摘Polypropylene(PP)scaffolds are the most commonly used biomedical scaffolds despite their disadvan-tages,which include problems with adhesion,infection,and inflammatory responses.Here,we report on the successful development of a facile one-step method to fabricate a series of novel triclosan poly-dopamine polypropylene(TPP)composite scaffolds and thereby effectively improve the biocompatibility and long-term antibacterial properties of PP scaffolds.The antibacterial triclosan can effectively interact with dopamine during biocompatible polydopamine formation on the PP scaffold by one-step green fab-rication.Thanks to the sustained release of triclosan from the biocompatible polydopamine coating,a 5mm×5mm sample of TPP-coated scaffold made with a triclosan concentration of 8 mg-mL^(-1)(referredto herein as TPP-8)exhibited a continuous antibacterial effect against both Escherichia coli(E.coli)and Staphylococcus aureus(S.aureus)for more than 15d,at maximum antibacterial volumes of 2 and 5mL,respectively.Our study establishes a new direction for facile long-term antibacterial studies for medical applications.
基金supported by grants from the National Natural Science Foundation of China[No.82273360]the State’s Key Project of Research and Development Plan[No.2019YFE0113700]+9 种基金the Guangdong Provincial Key Laboratory of Precision Medicine for Gastrointestinal Cancer[No.2020B121201004]the Guangdong Provincial Major Talents Project[No.2019JC05Y361]the China Postdoctoral Science Foundation[No.2020M682789]the Natural Science Foundation of Fujian Province[No.2018J07004]the Joint Funds of Fujian Provincial Health and Education Research[No.2019-WJ-21]the Science and Technology Program of Fujian Province[No.2018Y2003,2019L3018,2019YZ016006]the Clinical Research Startup Program of Southern Medical University by High-level University Construction Funding of Guangdong Provincial Department of Education[No.LC2016PY010]the Clinical Research Project of Nanfang Hospital[No.2018CR034,2020CR001,2020CR011]the President Foundation of Nanfang Hospital,Southern Medical University[No.2019Z023]the Training Program for Undergraduate Innovation and Entrepreneurship[No.201912121008,202012121091,202012121277].
文摘Background A significant difference in the anastomotic leakage(AL)rate has been observed between patients with locally advanced rectal cancer who have undergone preoperative chemotherapy and those undergoing preoperative chemoradiotherapy.This study aimed to quantitatively analyse collagen structural changes caused by preoperative chemoradiotherapy and illuminate the relationship between collagen changes and AL.Methods Anastomotic distal and proximal“doughnut”specimens from the Sixth Affiliated Hospital of Sun Yat-sen University(Guangzhou,China)were quantitatively assessed for collagen structural changes between patients with and without preoperative radiotherapy using multiphoton imaging.Then,patients treated with preoperative chemoradiotherapy were used as a training cohort to construct an AL–SVM classifier by the Mann–Whitney U test and support vector machine(SVM).An independent test cohort from the Fujian Province Cancer Hospital(Fuzhou,China)was used to validate the AL–SVM classifier.Results A total of 207 patients were included from the Sixth Affiliated Hospital of Sun Yat-sen University.The AL rate in the preoperative chemoradiotherapy group(n=107)was significantly higher than that in the preoperative chemotherapy group(n=100)(21.5%vs 7.0%,P=0.003).A fully quantitative analysis showed notable morphological and spatial distribution feature changes in collagen in the preoperative chemoradiotherapy group.Then,the patients who received preoperative chemoradiotherapy were used as a training cohort to construct the AL–SVM classifier based on five collagen features and the tumor distance from the anus.The AL–SVM classifier showed satisfactory discrimination and calibration with areas under the curve of 0.907 and 0.856 in the training and test cohorts,respectively.Conclusions The collagen structure may be notably altered by preoperative radiotherapy.The AL–SVM classifier was useful for the individualized prediction of AL in rectal cancer patients undergoing preoperative chemoradiotherapy.
