Highly-dispersed BaLuFs：yb3＋/Er3＋ nanocrystals were prepared by a facile potassium sodium tartrate- assisted hydrothermal method. The average particle size was approximately 20-25 nm. The formation mechanism is dis...Highly-dispersed BaLuFs：yb3＋/Er3＋ nanocrystals were prepared by a facile potassium sodium tartrate- assisted hydrothermal method. The average particle size was approximately 20-25 nm. The formation mechanism is discussed. Potassium sodium tartrate led to form a complex with an approximately three- dimensional network structure, which insured largely concurrent nucleation. As a result, we acquired uniform nanoparticles. The hydrothermal temperature, holding time, and pH value were important fac- tors affecting the formation of the BaLuF5 ：yb3＋/Er3＋ nanocrystals. We investigated their influence on the formation and realized the optimal reaction parameters. Remarkably, potassium sodium tartrate also con- tributed to the biocompatibility and potential biomedical applications of BaLuFs ：Yb3＋/Er3＋ nanocrystals by decomposing into small organic groups attached to the nanoparticles.展开更多
Senile plaque blue autofluorescence was discovered around 40 years ago,however,its impact on Alzheimer’s disease(AD)pathology has not been fully examined.We analyzed senile plaques with immunohistochemistry and fluor...Senile plaque blue autofluorescence was discovered around 40 years ago,however,its impact on Alzheimer’s disease(AD)pathology has not been fully examined.We analyzed senile plaques with immunohistochemistry and fluorescence imaging on AD brain sections and also Aβ aggregation in vitro.In DAPI or Hoechst staining,the nuclear blue fluorescence could only be correctly assigned after subtracting the blue plaque autofluorescence.The flower-like structures wrapping dense-core blue fluorescence formed by cathepsin D staining could not be considered central-nucleated neurons with defective lysosomes since there was no nuclear staining in the plaque core when the blue autofluorescence was subtracted.Both Aβ self-oligomers and Aβ/hemoglobin heterocomplexes generated blue autofluorescence.The Aβ amyloid blue autofluorescence not only labels senile plaques but also illustrates red cell aggregation,hemolysis,cerebral amyloid angiopathy,vascular plaques,vascular adhesions,and microaneurysms.In summary,we conclude that Aβ-aggregation-generated blue autofluorescence is an excellent multi-amyloidosis marker in Alzheimer’s disease.展开更多
文摘Highly-dispersed BaLuFs：yb3＋/Er3＋ nanocrystals were prepared by a facile potassium sodium tartrate- assisted hydrothermal method. The average particle size was approximately 20-25 nm. The formation mechanism is discussed. Potassium sodium tartrate led to form a complex with an approximately three- dimensional network structure, which insured largely concurrent nucleation. As a result, we acquired uniform nanoparticles. The hydrothermal temperature, holding time, and pH value were important fac- tors affecting the formation of the BaLuF5 ：yb3＋/Er3＋ nanocrystals. We investigated their influence on the formation and realized the optimal reaction parameters. Remarkably, potassium sodium tartrate also con- tributed to the biocompatibility and potential biomedical applications of BaLuFs ：Yb3＋/Er3＋ nanocrystals by decomposing into small organic groups attached to the nanoparticles.
基金supported by the National Natural Science Foundation of China(81472235)the Shanghai Jiao Tong University Medical and Engineering Project(YG2021QN53,YG2017MS71)+1 种基金the International Cooperation Project of National Natural Science Foundation of China(82020108017)the Innovation Group Project of National Natural Science Foundation of China(81921002).
文摘Senile plaque blue autofluorescence was discovered around 40 years ago,however,its impact on Alzheimer’s disease(AD)pathology has not been fully examined.We analyzed senile plaques with immunohistochemistry and fluorescence imaging on AD brain sections and also Aβ aggregation in vitro.In DAPI or Hoechst staining,the nuclear blue fluorescence could only be correctly assigned after subtracting the blue plaque autofluorescence.The flower-like structures wrapping dense-core blue fluorescence formed by cathepsin D staining could not be considered central-nucleated neurons with defective lysosomes since there was no nuclear staining in the plaque core when the blue autofluorescence was subtracted.Both Aβ self-oligomers and Aβ/hemoglobin heterocomplexes generated blue autofluorescence.The Aβ amyloid blue autofluorescence not only labels senile plaques but also illustrates red cell aggregation,hemolysis,cerebral amyloid angiopathy,vascular plaques,vascular adhesions,and microaneurysms.In summary,we conclude that Aβ-aggregation-generated blue autofluorescence is an excellent multi-amyloidosis marker in Alzheimer’s disease.
