参苓白术散治疗老年男性肌少症的成本-效用分析

目的评价参苓白术散治疗老年男性肌少症的成本-效用。方法280例老年男性肌少症患者随机分为干预组和对照组各140例,两组患者均给予营养运动治疗,干预组在营养运动治疗基础上加用参苓白术散,两组患者均随访24周。从卫生体系角度出发,利用TreeAge Pro 2011软件建立决策树模型,对两种治疗方案进行成本-效用分析,并采用敏感性分析对结果的不确定性进行评价。结果与对照组相比,干预组方案成本(5019.46元vs.4864.57元)、效用[0.90357质量调整生命年(QALYs)vs.0.89430 QALYs]均较高,增量成本-效用比为16712.05元/QALY,低于意愿支付阈值。敏感性分析结果与成本-效用分析结果一致。结论与单独应用营养运动治疗相比,老年男性肌少症患者应用参苓白术散联合营养运动治疗更具有成本-效用。

Inhibition effect of miR-577 on hepatocellular carcinoma cell growth via targeting β-catenin

Objective:To investigate the expression and the regulation effect of cell growth of microRNA-577 in hepatocellular carcinoma(HCC).Methods:qRT-PCR was applied to detect the relative expression of miR-577 in 70 paired HCC and matched tumor adjacent tissues collecting from resection between March 2011 and March 2014.Pearson chi-square test was used to analyze the relationship between the miR-577 expression and clinical features.The miR-577 mimics were transfected into HepG2 cells:cell cycles were detected by flow cytometry,cell proliferation was measured by MTT assay and BrdU incorporation assay,and cell apoptosis was determined by flow cytometry and Caspase 3/7 activity analysis.The expressions ofβ-catenin were measured by immunohistochemistry.Spearman correlation analysis was used to analyze the relationship between miR-577 and p-catenin.qRT-PCR and westernblot were used to detect the expression of p-catenin in transfected HepG2 cells.Results:The relative expressions of miR-577 was significantly lower in HCC tissues compared to the matched normal tumor-adjacent tissues(P<0.05).Low expression of miR-577 was significantly associated with large tumor size(≥5 cm,P<0.05) and advanced tumor node metastasis stage(Ⅲ+Ⅳ,P<0.05).Transfection of miR-577 mimics could inhibit repress cell proliferation,enhance cell apoptosis and block the cell cycles in G_1/G_1 phase(P<0.05).miR-577 in HCC group had a significant negative correlation relationship with the expression of downstream target of β-catenin(P<0.05).Both the mRNA and protein expression in HepG2 cells were down-regulated after transfection(P<0.05).Conclusions:Low expression of miR-577 is related to the malignant clinicopathological features in HCC tissues,and miR-577 may suppress HCC growth through down-regulating p-catenin.