Effect of Xinglou Chengqi Decoction on the blood brain barrier permeability, nerve damage and neurotrophy in rats with cerebral hemorrhage

Objective: To study the effect of Xinglou Chengqi Decoction on the blood brain barrier permeability, nerve damage and neurotrophy in rats with cerebral hemorrhage. Methods:Adult male SD rats were chosen as the experimental animals and randomly divided into control group, model group and intervention group, model group were made into cerebral hemorrhage models, and intervention group were made into cerebral hemorrhage models and given intragastric administration of Xinglou Chengqi Decoction for intervention. After 14 d of intervention, the blood brain barrier permeability, the expression of apoptosis genes as well as the contents of oxidative products, inflammatory products and nerve cytokines in brain tissues were determined. Results: The blood brain barrier permeability parameter EB level in brain tissue of model group was significantly higher than that of control group, and Bax, FasL, Fas and Caspase-3 mRNA expression as well as MDA, AOPP, TNF-α, HMGB1 and MCP-1 contents in brain tissue were significantly higher than those of control group whereas BDNF, NTF, VEGF and IGF-1 contents were significantly lower than those of control group;the blood brain barrier permeability parameter EB level in brain tissue of intervention group was significantly lower than that of model group, and Bax, FasL, Fas and Caspase-3 mRNA expression as well as MDA, AOPP, TNF-α, HMGB1 and MCP-1 contents in brain tissue were significantly lower than those of model group whereas BDNF, NTF, VEGF and IGF-1 contents were significantly higher than those of model group. Conclusion: Xinglou Chengqi Decoction intervention can reduce the blood brain barrier permeability, relieve the nerve damage and improve the neurotrophy in rat model with cerebral hemorrhage.

Correlation of the changes of blood brain barrier permeability with the activation of apoptosis, inflammatory response and stress response in rats with cerebral hemorrhage

Objective:To study the correlation of the changes of blood brain barrier permeability with the activation of apoptosis, inflammatory response and stress response in rats with cerebral hemorrhage.Methods: SD rats were chosen as experimental animals and divided into the cerebral hemorrhage group and the control group. 24 h after molding, formamide soaking method was referred to determine the levels of blood brain barrier permeability parameters as well as the contents of apoptosis molecules, inflammatory response molecules and oxidative stress molecules in brain tissues around the hematoma.Results: Blood brain barrier permeability parameter EB level in brain tissues around hematoma of cerebral hemorrhage group was significantly higher than that of control group, and DKK1, PAR-1, Bax, Cyt-C, NF-κB, ICAM1, VCAM1, TNF-α, MMP9, NOX4 and MDA contents were higher than those of control group, andβ-catenin, Bcl-2, Mn-SOD, GSH and T-AOC contents were lower than those of control group;Pearson correlation analysis showed that EB level in brain tissues around hematoma of cerebral hemorrhage group was positively correlated with DKK1, PAR-1, Bax, Cyt-C, NF-κB, ICAM1, VCAM1, TNF-α, MMP9, NOX4 and MDA contents, and negatively correlated withβ-catenin, Bcl-2, Mn-SOD, GSH and T-AOC contents. Conclusion:The increase of blood brain barrier permeability in rats with cerebral hemorrhage is related to the excessive activation of apoptosis, inflammatory response and stress response.