目的:抑郁症已成为继心血管疾病、糖尿病、肿瘤之后的第四大疾病,严重影响人类生活质量。本文着重探讨人参皂苷Rg1对嗅球摘除抑郁症大鼠模型的抗炎和神经保护作用及其机制。方法:大鼠分为假手术组(对照组)、造模组和治疗组,通过手术摘...目的:抑郁症已成为继心血管疾病、糖尿病、肿瘤之后的第四大疾病,严重影响人类生活质量。本文着重探讨人参皂苷Rg1对嗅球摘除抑郁症大鼠模型的抗炎和神经保护作用及其机制。方法:大鼠分为假手术组(对照组)、造模组和治疗组,通过手术摘除建立嗅球摘除抑郁症模型,治疗组术后给予人参皂苷Rg1。高效液相色谱法检测海马组织5-羟色胺(5-HT)浓度。CCK-8实验检测细胞活力。流式细胞术分析细胞凋亡。蛋白印迹法检测5-HT1A受体、增殖标记蛋白细胞增殖核抗原-67(Ki-67)和增殖细胞核抗原(PCNA)、凋亡标记蛋白caspase-3和caspase-9以及炎症因子IL-4、IL-10、IL-13、IL-6、IL-1β和TNF-α的表达。结果:造模组海马组织5-HT的浓度和5-HT1A受体表达远低于假手术组( P <0.01),治疗组海马组织5-HT的浓度和5-HT1A受体表达高于造模组( P <0.05);造模组神经胶质细胞活力远远低于假手术组( P <0.001)。治疗组神经胶质细胞活力显著升高( P <0.01);造模组神经胶质细胞凋亡率远高于假手术组( P <0.001)。治疗组细胞凋亡率显著低于造模组( P <0.01);与假手术组相比,造模组增殖标记蛋白Ki-67 和PCNA及抗炎因子IL-4、IL-10和IL-13的相对表达量大大减少;凋亡标记蛋白caspase-3 和caspase-9及促炎因子IL-6、IL-1β和TNF-α的相对表达量明显升高( P <0.05);与造模组相比,治疗组增殖标记蛋白Ki-67 和PCNA及抗炎因子造模组IL-4、IL-10和IL-13的相对表达量明显增加;凋亡标记蛋白caspase-3 和caspase-9及促炎因子IL-6、IL-1β和TNF-α的相对表达量明显降低( P <0.05)。结论:人参皂苷Rg1在抑郁症中可通过抗细胞凋亡起到神经保护的作用,并通过调控炎症因子的表达来发挥抗炎作用。展开更多
Salt plays a crucial role in food processing and consumption,and the rapid detection of chloride ions in food and feed has great significance for practical applications.In this work,Ag-based nanomaterials were deposit...Salt plays a crucial role in food processing and consumption,and the rapid detection of chloride ions in food and feed has great significance for practical applications.In this work,Ag-based nanomaterials were deposited on the surface of a flexible integrated electrochemical sensor for the detection of Cl-in food.In order to enhance the detection performance,a unique needle-tip structure was formed by manipulating the electro-engraving process during the electrodeposition growth.Theoretical calculations and electrochemical investigations have demonstrated that the dendrimer’s rich tip structure significantly enhanced its electrochemical performance.A sensitive and flexible integrated electrochemical sensor was creatively developed for the detection of Cl-using needletip effect-promoted Ag micro dendrimers.The sensor achieved quantitative detection of Cl-over a dynamic range of 10.0μM–100.0 mM,with a low limit of detection of 0.148μM.The flexible electrochemical sensor proposed in this work exhibited good repeatability,selectivity and recoveries in real food samples.展开更多
文摘目的:抑郁症已成为继心血管疾病、糖尿病、肿瘤之后的第四大疾病,严重影响人类生活质量。本文着重探讨人参皂苷Rg1对嗅球摘除抑郁症大鼠模型的抗炎和神经保护作用及其机制。方法:大鼠分为假手术组(对照组)、造模组和治疗组,通过手术摘除建立嗅球摘除抑郁症模型,治疗组术后给予人参皂苷Rg1。高效液相色谱法检测海马组织5-羟色胺(5-HT)浓度。CCK-8实验检测细胞活力。流式细胞术分析细胞凋亡。蛋白印迹法检测5-HT1A受体、增殖标记蛋白细胞增殖核抗原-67(Ki-67)和增殖细胞核抗原(PCNA)、凋亡标记蛋白caspase-3和caspase-9以及炎症因子IL-4、IL-10、IL-13、IL-6、IL-1β和TNF-α的表达。结果:造模组海马组织5-HT的浓度和5-HT1A受体表达远低于假手术组( P <0.01),治疗组海马组织5-HT的浓度和5-HT1A受体表达高于造模组( P <0.05);造模组神经胶质细胞活力远远低于假手术组( P <0.001)。治疗组神经胶质细胞活力显著升高( P <0.01);造模组神经胶质细胞凋亡率远高于假手术组( P <0.001)。治疗组细胞凋亡率显著低于造模组( P <0.01);与假手术组相比,造模组增殖标记蛋白Ki-67 和PCNA及抗炎因子IL-4、IL-10和IL-13的相对表达量大大减少;凋亡标记蛋白caspase-3 和caspase-9及促炎因子IL-6、IL-1β和TNF-α的相对表达量明显升高( P <0.05);与造模组相比,治疗组增殖标记蛋白Ki-67 和PCNA及抗炎因子造模组IL-4、IL-10和IL-13的相对表达量明显增加;凋亡标记蛋白caspase-3 和caspase-9及促炎因子IL-6、IL-1β和TNF-α的相对表达量明显降低( P <0.05)。结论:人参皂苷Rg1在抑郁症中可通过抗细胞凋亡起到神经保护的作用,并通过调控炎症因子的表达来发挥抗炎作用。
基金financially supported by Key Research and Development Program in Hubei Province(Grant No.2023BBB042)the Fundamental Research Funds for the Central Universities(Grant No.2662023GXQD001)Key Laboratory of on Site Processing Equipment for Agricultural Products,Ministry of Agriculture and Rural Affairs(Grant No.2016NYZD2202).
文摘Salt plays a crucial role in food processing and consumption,and the rapid detection of chloride ions in food and feed has great significance for practical applications.In this work,Ag-based nanomaterials were deposited on the surface of a flexible integrated electrochemical sensor for the detection of Cl-in food.In order to enhance the detection performance,a unique needle-tip structure was formed by manipulating the electro-engraving process during the electrodeposition growth.Theoretical calculations and electrochemical investigations have demonstrated that the dendrimer’s rich tip structure significantly enhanced its electrochemical performance.A sensitive and flexible integrated electrochemical sensor was creatively developed for the detection of Cl-using needletip effect-promoted Ag micro dendrimers.The sensor achieved quantitative detection of Cl-over a dynamic range of 10.0μM–100.0 mM,with a low limit of detection of 0.148μM.The flexible electrochemical sensor proposed in this work exhibited good repeatability,selectivity and recoveries in real food samples.
