MXD3表达水平与前列腺癌患者预后和免疫浸润的相关性研究

目的 探究MAX二聚化蛋白3(MXD3)在前列腺癌中的表达及与患者预后和免疫浸润的相关性。方法从TCGA数据库获取前列腺癌样本和正常样本的RNA测序数据和临床信息,比较肿瘤和相邻正常组织之间的MXD3表达水平。进行Kaplan-Meier生存分析,评估MXD3表达水平的预后价值和MXD3和临床特征之间的关联。采用GO和KEGG进行基因富集分析,使用ESTIMATE算法分析前列腺癌中MXD3表达与免疫细胞浸润之间的相关性。通过qRT-PCR和蛋白质印迹验证前列腺癌组织中的MXD3 mRNA和蛋白质表达水平。结果MXD3的表达水平在前列腺癌中显著高于正常前列腺组织,其高表达与不良预后显著相关,而且MXD3表达水平与肿瘤的临床分期呈正相关(P<0.05或P<0.001)。GO功能和KEGG通路富集分析结果显示MXD3与前列腺癌细胞的分裂增殖密切相关。MXD3的表达与调节性T细胞和滤泡辅助T细胞的免疫细胞浸润水平呈正相关(P<0.001和P<0.05),而与肥大细胞和CD4^(+)T细胞呈负相关(P<0.01和P<0.001)。与癌旁正常组织相比,MXD3在前列腺癌中mRNA和蛋白质表达显著增加(P<0.01)。结论 MXD3在前列腺癌中高表达,与前列腺癌患者不良预后有关,参与前列腺癌的分裂增殖并与免疫浸润相关,是潜在的预后生物标志物和免疫靶点。

Regulatory Effect of Electroacupuncture on Ceramide Galactosyltransferase Expression in Sciatic Nerve of Experimental Diabetic Rats

Objective: To observe the change of ceramide galactosyltransferase(CGT) expression in sciatic nerve of experimental diabetic rats after electroacupuncture(EA) treatment. Methods: The rat model of diabetic peripheral neuropathy(DPN) was established with Streptozotocin(STZ). Twenty-eight rats were randomly divided into a normal group, a model group, an EA acupoint group, and an EA non-acupoint group, with 7 rats in each group. Acupuncture on points Shenshu(BL 23) and Zusanli(ST 36) was performed in the EA group using EA electronic device with continuous wave, 2 Hz, 20 min every time, once every other day for 12 times. Acupuncture(on the tip of rat tail) in EA non-acupoint group was performed with the same electronic parameters and time. The model group and the normal group were not given any treatment, except the same fixation as that in the EA acupoint group. After 12 treatments, the albumen and mRNA expressions of CGT of sciatic nerve in rats from each group were measured with real-time polymerase chain reaction(RT-PCR), fluorescence quantitative PCR(FQ-PCR) and immunohistochemical methods. Results: After modeling, the CGT expression was significantly lower than that in the normal group(P<0.01); however, after 12 EA treatments, the CGT positive cells in the EA acupoint group were up-regulated and significantly higher than those in the EA non-acupoint group and the model group(P<0.01). The CGT mRNA expression of the rats after modeling was also higher than that of the normal rats(P<0.01), and the expression in the EA acupoint group was significantly lower than those in the model group and the EA non-acupoint group(P<0.01, P<0.05). Conclusion: EA treatment with acupoints displays some therapeutic or preventive effect in the recovery of DPN via reversing the abnormal expression of CGT mRNA in sciatic nerves of experimental diabetic neuropathy rats, and it is worthy of further study.