Ths paper,based on the principles of geometric self-similarity of fractal theory and some research results of rotein chemistry,improved the method of comput-ing protein fractal dimensions,and computed fractal dime...Ths paper,based on the principles of geometric self-similarity of fractal theory and some research results of rotein chemistry,improved the method of comput-ing protein fractal dimensions,and computed fractal dimensions of some protein back bone,secondary and assumed folding structures.The relationship between protein back-bone strucrural fractal dimensions and its spatial structures was investigated.The results indicated that protein backbone fractal dimensions not only have a close relation with protein secondary structure,but also with its folding.In addition,the folding of protein Polypeptide chains in 3-D space may be similar to the other macromolecular chain be haviour described by the self-avoiding walks(SAW)model.展开更多
文摘目的:子宫内膜癌是女性生殖系统常见的恶性肿瘤,以子宫内膜样癌(endometrioid adenocarcinoma,EEC)最常见,其发病机制目前尚不清楚,已有研究证明环状RNA(circular RNA,circRNA)的表达与EEC的进展有关。本研究旨在检测hsa_circ_0007067在EEC中的表达,并探讨Hsa_circ_0007067与EEC临床病理特征的关系。方法:应用高通量测序分析2例EEC组织和2例正常子宫内膜组织基因表达水平,以|log2(Fold changes)|≥1.5且P<0.05为筛选标准,选择下调最显著的hsa_circ_0007067为研究对象,应用实时聚合酶链反应(real-time PCR)检测36例EEC组织(EEC组)和36例正常子宫内膜组织(对照组)中hsa_circ_0007067的相对表达量。生物信息学分析预测hsa_circ_0007067的结合位点和编码能力。结果:EEC组中hsa_circ_0007067表达水平显著低于对照组(P<0.05),且表达水平与国际妇产科联盟(Inter-national Federation of Gynecology and Obstetrics,FIGO)分期和组织分化程度有关;受试者操作特征(receiver operating characteristic,ROC)曲线显示曲线下面积(area under curve,AUC)为0.936,敏感度为0.833,特异度为0.889,最佳截断值为0.722,差异具有统计学意义(P<0.05)。Hsa_circ_0007067具有翻译成多肽的潜能,circbank数据库预测其miRNA潜在结合位点共24个。结论:Hsa_circ_0007067与EEC的发生、发展相关,可能通过与下游靶基因miRNA结合或编码蛋白质功能影响EEC的进程,有望作为EEC早期筛查和判断预后的分子指标。
文摘Ths paper,based on the principles of geometric self-similarity of fractal theory and some research results of rotein chemistry,improved the method of comput-ing protein fractal dimensions,and computed fractal dimensions of some protein back bone,secondary and assumed folding structures.The relationship between protein back-bone strucrural fractal dimensions and its spatial structures was investigated.The results indicated that protein backbone fractal dimensions not only have a close relation with protein secondary structure,but also with its folding.In addition,the folding of protein Polypeptide chains in 3-D space may be similar to the other macromolecular chain be haviour described by the self-avoiding walks(SAW)model.