Effect of Sweet Corn Straw Returning to the Field on Soil Fertility, Yield and Benefit

It is an important way for realizing sustainable development of sweet corn production to stabilize and improve soil fertility of cultivated land in sweet corn production region.Through the test of sweet corn straw directly returning to the field after 6seasons for 3years,the results showed that continuous single application of chemical fertilizer is not conducive to the stability of soil fertility and yield improvement,and implementation of straw returning could receive fertility,improve soil acidic conditions,and enhance the yield of sweet corn.Compared with before the test,the single application of chemical fertilizer increased soil available phosphorus,while the contents of soil organic matter,available nitrogen and available potassium decreased by 1.08,1.18 and 2.47mg/kg respectively,and the soil pH decreased by 0.15.Under the same fertilizer conditions,organic matter contents of single and double-season straw returning increased by 0.71 and 1.29g/kg,available nitrogen increased by 17.15 and 28.27mg/kg,available phosphorus increased by 0.96 and 1.73mg/kg,available potassium increased by 2.41 and 5.92mg/kg,the soil pH increased by 0.16 and 0.2.Compared with the single application of chemical fertilizer,the average yields of single and double-season straw returning increased by 7.5%and 11.8%,and their average income increased by 87.3and 117.1yuan of per mu(667m^2)respectively.

Screening and identification of Shigella flexneri 2a virulence-related genes induced after invasion of epithelial cells

An in vivo expression technology (IVET) was applied to screen s.flexneri 2a genes induced after invasion of epithelial cells, and virulence-related genes were further identified by mutational analysis. Thirteen intracellular induced genes were identified with a HeLa cell infection model. Of these, two were identified as alkylation-related genes; one was related to metabolism; one encoded a transcriptional regulator; three were identified as insertion elements; three ap- peared to be antisense to genes involved in the transmethylation,biosyntheseis, and phos- photransferase system;and three were predicted to encode polypeptides with unknown functions. Intracellular survival assavs showed that the mutants of alkA,citC and wcaJ genes had lower capability of intracellular replication or survival than the the wild-type strain.The results indicated that alkA, citC and wcaJ genes could take part in the intracellular survival or replication of S. flexneri 2a and the capability of intracellular survival or replication could be one of the major virulence elements. However, the yaiC mutant was able to survive in the murine infection assay but almost not in HeLa cell infection assay. Very possibly, yaiC gene was involved in the other mechanism of S. flexneri virulence. This study might lead to a better understanding of the intra- cellular survival or proliferation process of S. flexneri 2a and perhaps provide insights into the pathogenicity of this pathogen.