Dear Editor,With the inevitable trend of global warming,it is urgent to understand how plants sense and respond to temperature increases for designing new crop varieties that can tolerate high ambient temperature.In A...Dear Editor,With the inevitable trend of global warming,it is urgent to understand how plants sense and respond to temperature increases for designing new crop varieties that can tolerate high ambient temperature.In Arabidopsis thaliana,high ambient temperature promotes hypocotyl elongation in seedlings and stimulates petiole elongation and hyponasty in rosette leaves.These changes in architecture are collectively tenned thermomorphogenesis.Thennomorphogenesis protects seedling meristems from the heat reflected from the ground and reduces the heat flux among leaves in adult plants.展开更多
High temperature activates the transcription factor PHYTOCHROME-INTERACTING FACTOR4(PIF4)to stimulate auxin signaling,which causes hypocotyl elongation and leaf hyponasty(thermomorphogenesis).HOOKLESS1(HLS1)is a recen...High temperature activates the transcription factor PHYTOCHROME-INTERACTING FACTOR4(PIF4)to stimulate auxin signaling,which causes hypocotyl elongation and leaf hyponasty(thermomorphogenesis).HOOKLESS1(HLS1)is a recently reported positive regulator of thermomorphogenesis,but the molecular mechanisms by which HLS1 regulates thermomorphogenesis remain unknown.In this study,we initially compared PIF4-and/or HLS1-dependent differential gene expression(DEG)upon high-temperature treatment.We found that a large number of genes are coregulated by PIF4 and HLS1,especially genes involved in plant growth or defense responses.Moreover,we found that HLS1 interacts with PIF4 to form a regulatory module and that,among the HLS1-PIF4-coregulated genes,27.7%are direct targets of PIF4.We also identified 870 differentially alternatively spliced genes(DASGs)in wild-type plants under high temperature.Interestingly,more than half of these DASG events(52.4%)are dependent on both HLS1 and PIF4,and the spliceosome-defective mutant plantsexhibit a hyposensitive response to high temperature,indicating that DASGs are required for thermomorphogenesis.Further comparative analyses showed that the HLS1/PIF4-coregulated DEGs and DASGs exhibit almost no overlap,suggesting that high temperature triggers two distinct strategies to control plant responses and thermomorphogenesis.Taken together,these results demonstrate that the HLS1-PIF4 module precisely controls both transcriptional and posttranscriptional regulation during plant thermomorphogenesis.展开更多
基金supported by the National Natural Science Foundation of China (31470375)the Fok Ying Tong Education Foundation (161023)+1 种基金the open funds of the State Key Laboratory of Plant Physiology and Biochemistry (SKLPPBKF1802)the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘Dear Editor,With the inevitable trend of global warming,it is urgent to understand how plants sense and respond to temperature increases for designing new crop varieties that can tolerate high ambient temperature.In Arabidopsis thaliana,high ambient temperature promotes hypocotyl elongation in seedlings and stimulates petiole elongation and hyponasty in rosette leaves.These changes in architecture are collectively tenned thermomorphogenesis.Thennomorphogenesis protects seedling meristems from the heat reflected from the ground and reduces the heat flux among leaves in adult plants.
基金This work was supported by the National Natural Science Foundation of China(31970256)the Fok Ying Tong Education Foundation(161023)+1 种基金the Fundamental Research Funds for the Central Universities(lzujbky-2019-kb05)the Priority Academic Program Development of Jiangsu Higher Education Institutions.
文摘High temperature activates the transcription factor PHYTOCHROME-INTERACTING FACTOR4(PIF4)to stimulate auxin signaling,which causes hypocotyl elongation and leaf hyponasty(thermomorphogenesis).HOOKLESS1(HLS1)is a recently reported positive regulator of thermomorphogenesis,but the molecular mechanisms by which HLS1 regulates thermomorphogenesis remain unknown.In this study,we initially compared PIF4-and/or HLS1-dependent differential gene expression(DEG)upon high-temperature treatment.We found that a large number of genes are coregulated by PIF4 and HLS1,especially genes involved in plant growth or defense responses.Moreover,we found that HLS1 interacts with PIF4 to form a regulatory module and that,among the HLS1-PIF4-coregulated genes,27.7%are direct targets of PIF4.We also identified 870 differentially alternatively spliced genes(DASGs)in wild-type plants under high temperature.Interestingly,more than half of these DASG events(52.4%)are dependent on both HLS1 and PIF4,and the spliceosome-defective mutant plantsexhibit a hyposensitive response to high temperature,indicating that DASGs are required for thermomorphogenesis.Further comparative analyses showed that the HLS1/PIF4-coregulated DEGs and DASGs exhibit almost no overlap,suggesting that high temperature triggers two distinct strategies to control plant responses and thermomorphogenesis.Taken together,these results demonstrate that the HLS1-PIF4 module precisely controls both transcriptional and posttranscriptional regulation during plant thermomorphogenesis.
