Background:Consumption of resistant starch(RS)has been associated with various intestinal and systemic health benefits,but knowledge of its effects on intestinal health and inflammatory response in stressed birds is l...Background:Consumption of resistant starch(RS)has been associated with various intestinal and systemic health benefits,but knowledge of its effects on intestinal health and inflammatory response in stressed birds is limited.Here,we examined how dietary RS supplementation from 12%raw potato starch(RPS)modulated inflammatory severity induced by lipopolysaccharide(LPS)in meat ducks.Results:LPS administration at 14,16,and 18 d(chronic challenge)decreased body weight(BW)and glucagon-like peptide 1 receptor(GLP-1R)level with higher intestinal permeability and inflammation,evident by higher proinflammatory cytokine levels.Dietary 12%RPS supplementation enhanced Claudin-1 and GLP-1R expression,along with lower levels of inflammatory factors in both ileum and serum.Microbiome analysis showed that RS treatment shifted microbial structure reflected by enriched the proportion of Firmicutes,Bifidobacterium,Ruminococcus,etc.Dietary RS addition also significantly increased the concentrations of propionate and butyrate during chronic LPS challenge.Furthermore,response to acute challenge,the ducks received 2 mg/kg BW LPS at 14 d had higher concentrations of serum endotoxins and inflammatory cytokines,as well as upregulated transcription of toll like receptor 4(TLR4)in ileum when compared to control birds.Analogous to GLP-1 agonist liraglutide,dietary RS addition decreased endotoxins and inflammation cytokines,whereas it upregulated the GLP-1 synthesis related genes expression.Meanwhile,dietary RS supplementation suppressed the acute LPS challenge-induced TLR4 transcription.Conclusions:These data suggest that dietary 12%RPS supplementation could attenuate the LPS-induced inflammation as well as intestinal injury of meat ducks,which might involve in the alteration in gut microbiota,SCFAs production and the signaling pathways of TLR4 and GLP-1/GLP-1R.展开更多
The influence of nanocrystalline cellulose(NCC) supplementation on growth performance, carcass traits,intestinal development, and lipid metabolism was assessed in 600 one-day-old male meat ducks(Cherry Valley ducks) f...The influence of nanocrystalline cellulose(NCC) supplementation on growth performance, carcass traits,intestinal development, and lipid metabolism was assessed in 600 one-day-old male meat ducks(Cherry Valley ducks) from 1 to 35 d of age. Diets were supplemented with 0,200,500,800 and 1,500 mg/kg NCC during both the starter(1-14 d) and grower(15-35 d) phases. Each dietary treatment consisted of 8 replicate cages of 15 birds. Supplementation of NCC was associated with dose dependent increases in BW gain and feed intake(P < 0.01) during 1-14 d of age and in BW at 35 d of age. As NCC content increased,the percentage of breast meat weight(P < 0.05) and leg(with bone) weight(P < 0.05) linearly increased,while the percentage of abdominal fat weight(P < 0.01) linearly decre.ased in ducks at 35 d of age.Supplementation of NCC resulted in a dose-dependent increase in the weight(P < 0.05) and density(P < 0.01) of the cecum. The percentage of total hepatic lipid content(P < 0.01) at 14 d of age and serum triglyceride(TG) concentration(P = 0.052) at 35 d of age linearly decreased with increasing of dietary NCC addition. In conclusion, inclusion of 1,500 mg/kg NCC in feed resulted in the greatest improvements in duck performance, intestinal development and lipid deposition.展开更多
基金supported by the National Natural Science Foundation of China(31772622)China Agriculture Research System(CARS-42-10)+2 种基金National Key R&D Program of China(2017YFD0502004)“111”project of Foreign Experts Affairs of ChinaSichuan Agricultural University 211 Foundation.
文摘Background:Consumption of resistant starch(RS)has been associated with various intestinal and systemic health benefits,but knowledge of its effects on intestinal health and inflammatory response in stressed birds is limited.Here,we examined how dietary RS supplementation from 12%raw potato starch(RPS)modulated inflammatory severity induced by lipopolysaccharide(LPS)in meat ducks.Results:LPS administration at 14,16,and 18 d(chronic challenge)decreased body weight(BW)and glucagon-like peptide 1 receptor(GLP-1R)level with higher intestinal permeability and inflammation,evident by higher proinflammatory cytokine levels.Dietary 12%RPS supplementation enhanced Claudin-1 and GLP-1R expression,along with lower levels of inflammatory factors in both ileum and serum.Microbiome analysis showed that RS treatment shifted microbial structure reflected by enriched the proportion of Firmicutes,Bifidobacterium,Ruminococcus,etc.Dietary RS addition also significantly increased the concentrations of propionate and butyrate during chronic LPS challenge.Furthermore,response to acute challenge,the ducks received 2 mg/kg BW LPS at 14 d had higher concentrations of serum endotoxins and inflammatory cytokines,as well as upregulated transcription of toll like receptor 4(TLR4)in ileum when compared to control birds.Analogous to GLP-1 agonist liraglutide,dietary RS addition decreased endotoxins and inflammation cytokines,whereas it upregulated the GLP-1 synthesis related genes expression.Meanwhile,dietary RS supplementation suppressed the acute LPS challenge-induced TLR4 transcription.Conclusions:These data suggest that dietary 12%RPS supplementation could attenuate the LPS-induced inflammation as well as intestinal injury of meat ducks,which might involve in the alteration in gut microbiota,SCFAs production and the signaling pathways of TLR4 and GLP-1/GLP-1R.
基金supported by grants from the National Scientific and Technical Supporting Program (2014BAD13B02)Meat Duck Industry Chain in Sichuan Province (2014NZ0030)Academy of Kechuang Feed Industry in Sichuan and Sichuan Agricultural University 211 Foundation of China
文摘The influence of nanocrystalline cellulose(NCC) supplementation on growth performance, carcass traits,intestinal development, and lipid metabolism was assessed in 600 one-day-old male meat ducks(Cherry Valley ducks) from 1 to 35 d of age. Diets were supplemented with 0,200,500,800 and 1,500 mg/kg NCC during both the starter(1-14 d) and grower(15-35 d) phases. Each dietary treatment consisted of 8 replicate cages of 15 birds. Supplementation of NCC was associated with dose dependent increases in BW gain and feed intake(P < 0.01) during 1-14 d of age and in BW at 35 d of age. As NCC content increased,the percentage of breast meat weight(P < 0.05) and leg(with bone) weight(P < 0.05) linearly increased,while the percentage of abdominal fat weight(P < 0.01) linearly decre.ased in ducks at 35 d of age.Supplementation of NCC resulted in a dose-dependent increase in the weight(P < 0.05) and density(P < 0.01) of the cecum. The percentage of total hepatic lipid content(P < 0.01) at 14 d of age and serum triglyceride(TG) concentration(P = 0.052) at 35 d of age linearly decreased with increasing of dietary NCC addition. In conclusion, inclusion of 1,500 mg/kg NCC in feed resulted in the greatest improvements in duck performance, intestinal development and lipid deposition.