Fallopia multiflora(Thunb.)Harald,a vine belonging to the Polygonaceae family,is used in traditional medicine.The stilbenes contained in it have significant pharmacological activities in anti-oxidation and anti-aging....Fallopia multiflora(Thunb.)Harald,a vine belonging to the Polygonaceae family,is used in traditional medicine.The stilbenes contained in it have significant pharmacological activities in anti-oxidation and anti-aging.This study describes the assembly of the F.multiflora genome and presents its chromosome-level genome sequence containing 1.46 gigabases of data(with a contig N50 of 1.97 megabases),1.44 gigabases of which was assigned to 11 pseudochromosomes.Comparative genomics confirmed that F.multiflora shared a whole-genome duplication event with Tartary buckwheat and then underwent different transposon evolution after separation.Combining genomics,transcriptomics,and metabolomics data to map a network of associated genes and metabolites,we identified two FmRS genes responsible for the catalysis of one molecule of p-coumaroyl-CoA and three molecules of malonyl-CoA to resveratrol in F.multiflora.These findings not only serve as the basis for revealing the stilbene biosynthetic pathway but will also contribute to the development of tools for increasing the production of bioactive stilbenes through molecular breeding in plants or metabolic engineering in microbes.Moreover,the reference genome of F.multiflora is a useful addition to the genomes of the Polygonaceae family.展开更多
Exploration of herbal medicine started upon the incipient of early agriculture[1],meanwhile,the processing methods have been continually improved for higher efficacy,reduced toxicity,and simplified application.The ear...Exploration of herbal medicine started upon the incipient of early agriculture[1],meanwhile,the processing methods have been continually improved for higher efficacy,reduced toxicity,and simplified application.The earliest written records of herbal processing can be found in Huangdi Neijing,but the descriptions are too brief to be illustrative.展开更多
基金This study was funded by the National Natural Science Foundation of China(No.81973432)the Major Increase and Reduction Project at the Central Level(No.2060302)+1 种基金Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine(ZYYCXTD-D-202005)CAMS Innovation Fund for Medical Sciences(2019-I2M-5-065).
文摘Fallopia multiflora(Thunb.)Harald,a vine belonging to the Polygonaceae family,is used in traditional medicine.The stilbenes contained in it have significant pharmacological activities in anti-oxidation and anti-aging.This study describes the assembly of the F.multiflora genome and presents its chromosome-level genome sequence containing 1.46 gigabases of data(with a contig N50 of 1.97 megabases),1.44 gigabases of which was assigned to 11 pseudochromosomes.Comparative genomics confirmed that F.multiflora shared a whole-genome duplication event with Tartary buckwheat and then underwent different transposon evolution after separation.Combining genomics,transcriptomics,and metabolomics data to map a network of associated genes and metabolites,we identified two FmRS genes responsible for the catalysis of one molecule of p-coumaroyl-CoA and three molecules of malonyl-CoA to resveratrol in F.multiflora.These findings not only serve as the basis for revealing the stilbene biosynthetic pathway but will also contribute to the development of tools for increasing the production of bioactive stilbenes through molecular breeding in plants or metabolic engineering in microbes.Moreover,the reference genome of F.multiflora is a useful addition to the genomes of the Polygonaceae family.
基金supported by the National Natural Science Foundation of China(81891013 and 81891010)Key Project at Central Government Level for the Ability Establishment of Sustainable Use for Valuable Chinese Medicine Resources(2060302)。
文摘Exploration of herbal medicine started upon the incipient of early agriculture[1],meanwhile,the processing methods have been continually improved for higher efficacy,reduced toxicity,and simplified application.The earliest written records of herbal processing can be found in Huangdi Neijing,but the descriptions are too brief to be illustrative.
