期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
SBA-15 Modified Carbon Paste Electrode for Rapid cTnI Detection with Enhanced Sensitivity
1
作者 Nong Yue HE hui shi guo +2 位作者 Di YANG Chun Rong GU Ji Nan ZHANG 《Chinese Chemical Letters》 SCIE CAS CSCD 2006年第2期235-238,共4页
A novel electrochemical immunoassay for cardiac troponin Ⅰ (cTnI) combining the concepts of the dual monoclonal antibody "sandwich" principle, the silver enhancement on the nano-gold particle, and the SBA-15 meso... A novel electrochemical immunoassay for cardiac troponin Ⅰ (cTnI) combining the concepts of the dual monoclonal antibody "sandwich" principle, the silver enhancement on the nano-gold particle, and the SBA-15 mesoporous modified carbon paste electrode (SBA-MCPE) is described. Four main steps were carried out to obtain the analytical signal, i.e., electrode preparation, immunoreaction, silver enhancement, and anodic stripping voltammetric detection. A linear relationship between the anodic stripping peak current and concentration of cTnI from 0.5 to 5.0 ng/mL and a limit of detection of 0.2 ng/mL of cTnI were obtained. 展开更多
关键词 Cardiac troponin SBA-15 mesoporous materials carbon paste electrode rapid quantitative immunoassay anodic stripping voltammetric detection NANO-GOLD sensitivity.
下载PDF
Application of Nanogoid Probe Coupled with Silver Enhancement in Rapid cTnI Colorimetric Immunoassay
2
作者 Nong Yue HE hui shi guo +4 位作者 Di YANG Chun Rong GU Zhi Ping BIAN Wen hui WAN Ji Nan ZHANG 《Chinese Chemical Letters》 SCIE CAS CSCD 2005年第12期1625-1628,共4页
A rapid, inexpensive, reliable, and flexible quantitative immunoassay for cardiac troponin I (cTnI) based on the concepts of one-step dual monoclonal antibody “sandwich” principle. The low density protein array, t... A rapid, inexpensive, reliable, and flexible quantitative immunoassay for cardiac troponin I (cTnI) based on the concepts of one-step dual monoclonal antibody “sandwich” principle. The low density protein array, the nanogold probe, and the silver enhancement on the gold particle were provided. The whole detection procedure of the assay could be fulfilled within 40 min with the pretreated colloidal gold-labeled detection antibody and supporting substrate. The assay showed good specific response to cTnI with very low cross-reactivity ratio to the skeletal isoforms of troponin I (sTnI), cardiac troponin T (cTnT), and myoglobin. 588 serum samples were assayed simultaneously by enzyme-linked immuno sorbent assay (ELISA) and this colloidal gold method to test the validity of the method and the data were analyzed using the statistical package SPSS version 11.0 (SPSS Inc.). There was no significant difference between these two assays (P=0.66〉0.05). The agreement between this method (〉 or 〈0.3 ng/mL) and ELISA was 86%. 展开更多
关键词 Cardiac troponin I protein array nanogold particles silver enhancement rapidquantitative immunoassay
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部