A novel electrochemical immunoassay for cardiac troponin Ⅰ (cTnI) combining the concepts of the dual monoclonal antibody "sandwich" principle, the silver enhancement on the nano-gold particle, and the SBA-15 meso...A novel electrochemical immunoassay for cardiac troponin Ⅰ (cTnI) combining the concepts of the dual monoclonal antibody "sandwich" principle, the silver enhancement on the nano-gold particle, and the SBA-15 mesoporous modified carbon paste electrode (SBA-MCPE) is described. Four main steps were carried out to obtain the analytical signal, i.e., electrode preparation, immunoreaction, silver enhancement, and anodic stripping voltammetric detection. A linear relationship between the anodic stripping peak current and concentration of cTnI from 0.5 to 5.0 ng/mL and a limit of detection of 0.2 ng/mL of cTnI were obtained.展开更多
A rapid, inexpensive, reliable, and flexible quantitative immunoassay for cardiac troponin I (cTnI) based on the concepts of one-step dual monoclonal antibody “sandwich” principle. The low density protein array, t...A rapid, inexpensive, reliable, and flexible quantitative immunoassay for cardiac troponin I (cTnI) based on the concepts of one-step dual monoclonal antibody “sandwich” principle. The low density protein array, the nanogold probe, and the silver enhancement on the gold particle were provided. The whole detection procedure of the assay could be fulfilled within 40 min with the pretreated colloidal gold-labeled detection antibody and supporting substrate. The assay showed good specific response to cTnI with very low cross-reactivity ratio to the skeletal isoforms of troponin I (sTnI), cardiac troponin T (cTnT), and myoglobin. 588 serum samples were assayed simultaneously by enzyme-linked immuno sorbent assay (ELISA) and this colloidal gold method to test the validity of the method and the data were analyzed using the statistical package SPSS version 11.0 (SPSS Inc.). There was no significant difference between these two assays (P=0.66〉0.05). The agreement between this method (〉 or 〈0.3 ng/mL) and ELISA was 86%.展开更多
文摘A novel electrochemical immunoassay for cardiac troponin Ⅰ (cTnI) combining the concepts of the dual monoclonal antibody "sandwich" principle, the silver enhancement on the nano-gold particle, and the SBA-15 mesoporous modified carbon paste electrode (SBA-MCPE) is described. Four main steps were carried out to obtain the analytical signal, i.e., electrode preparation, immunoreaction, silver enhancement, and anodic stripping voltammetric detection. A linear relationship between the anodic stripping peak current and concentration of cTnI from 0.5 to 5.0 ng/mL and a limit of detection of 0.2 ng/mL of cTnI were obtained.
基金supported by the fund of“135”key laboratory of Jiangsu provincethe high-tech research program of Jiangsu province+1 种基金the Chinese Post Doctoral Science Foundation,the National Natural Science Foundation of China(No.60571032)the Trans-Century Training Programme Foundaion for the Talents by the Ministry of Education of China.
文摘A rapid, inexpensive, reliable, and flexible quantitative immunoassay for cardiac troponin I (cTnI) based on the concepts of one-step dual monoclonal antibody “sandwich” principle. The low density protein array, the nanogold probe, and the silver enhancement on the gold particle were provided. The whole detection procedure of the assay could be fulfilled within 40 min with the pretreated colloidal gold-labeled detection antibody and supporting substrate. The assay showed good specific response to cTnI with very low cross-reactivity ratio to the skeletal isoforms of troponin I (sTnI), cardiac troponin T (cTnT), and myoglobin. 588 serum samples were assayed simultaneously by enzyme-linked immuno sorbent assay (ELISA) and this colloidal gold method to test the validity of the method and the data were analyzed using the statistical package SPSS version 11.0 (SPSS Inc.). There was no significant difference between these two assays (P=0.66〉0.05). The agreement between this method (〉 or 〈0.3 ng/mL) and ELISA was 86%.