According to the positive correlation of coal ash content and natural gamma, using a new coal core reposition method, which is ordered by global and local extreme, coal samples from medium-thickness seam are reasonabl...According to the positive correlation of coal ash content and natural gamma, using a new coal core reposition method, which is ordered by global and local extreme, coal samples from medium-thickness seam are reasonably located. Inte- grated the data of coal macrostructure characteristics, coal petrography analysis and coal gas production test, it studies the rela- tionship between coalbody structure and amplitude variation of different well logging data, and the tectonic coal recognition method with well logging data in fresh-water mud invasion. The results show that： the anomalous response of natural gamma ray, neutron, density and apparent resistivity does not reflect the coalbody structure type. In fresh-water drilling mud invasion, using the crossplot technique of dual-lateral, RXO resistivity response and the coalbody structure can classify granulated coal accurately; the proposed method is of good practicability and high reliability.展开更多
The sandwich-type lateral fl ow assays relying on dual aptamers with high sensitivity and specifi city have been broadly explored.However,it is unlikely to match a pair of specifi c aptamers that can bind a small mole...The sandwich-type lateral fl ow assays relying on dual aptamers with high sensitivity and specifi city have been broadly explored.However,it is unlikely to match a pair of specifi c aptamers that can bind a small molecular target(e.g.,cocaine)simultaneously due to the steric hindrance.In response,we herein introduced the strategy of“one divides into two”into the construction of sandwich-type lateral fl ow strip assay(LFSA).Specifi cally,we split a single cocaine-recognizing aptamer into two segments,either of which was conjugated with gold nanoparticles(AuNPs)or labeled with biotin,serving as signal probe and capture probe,respectively.Upon the presence of the target molecule,a ternary sandwich complex comprised of the two halves of the aptamer and the target formed.Such sandwich-type LFSA exhibited an excellent nonlinear logarithmic response in the range from 10μmol/L to 5 mmol/L with R^(2)=0.9994.The sensitive on-site detection of cocaine in artifi cial biological samples including urine and sweat was achieved within 15 min,with the visual limit of detection as low as 50μmol/L for urine and 200μmol/L for sweat,and the recovery rates of 83.6-107.4%.展开更多
文摘According to the positive correlation of coal ash content and natural gamma, using a new coal core reposition method, which is ordered by global and local extreme, coal samples from medium-thickness seam are reasonably located. Inte- grated the data of coal macrostructure characteristics, coal petrography analysis and coal gas production test, it studies the rela- tionship between coalbody structure and amplitude variation of different well logging data, and the tectonic coal recognition method with well logging data in fresh-water mud invasion. The results show that： the anomalous response of natural gamma ray, neutron, density and apparent resistivity does not reflect the coalbody structure type. In fresh-water drilling mud invasion, using the crossplot technique of dual-lateral, RXO resistivity response and the coalbody structure can classify granulated coal accurately; the proposed method is of good practicability and high reliability.
基金supported by the National Natural Science Foundation of China(22090050,22122410,21874121)the National Key Research and Development Program of China(2018YFE0206900)+1 种基金Hubei Provincial Natural Science Foundation of China(2020CFA037)Zhejiang Provincial Natural Science Foundation of China(LD21B050001)
文摘The sandwich-type lateral fl ow assays relying on dual aptamers with high sensitivity and specifi city have been broadly explored.However,it is unlikely to match a pair of specifi c aptamers that can bind a small molecular target(e.g.,cocaine)simultaneously due to the steric hindrance.In response,we herein introduced the strategy of“one divides into two”into the construction of sandwich-type lateral fl ow strip assay(LFSA).Specifi cally,we split a single cocaine-recognizing aptamer into two segments,either of which was conjugated with gold nanoparticles(AuNPs)or labeled with biotin,serving as signal probe and capture probe,respectively.Upon the presence of the target molecule,a ternary sandwich complex comprised of the two halves of the aptamer and the target formed.Such sandwich-type LFSA exhibited an excellent nonlinear logarithmic response in the range from 10μmol/L to 5 mmol/L with R^(2)=0.9994.The sensitive on-site detection of cocaine in artifi cial biological samples including urine and sweat was achieved within 15 min,with the visual limit of detection as low as 50μmol/L for urine and 200μmol/L for sweat,and the recovery rates of 83.6-107.4%.
