Plasma DNA methylation detection for early screening,diagnosis,and monitoring of esophageal adenocarcinoma and squamous cell carcinoma

BACKGROUND The early diagnosis rate of esophageal cancer(EC),one of the most prevalent digestive tract cancers worldwide,remains low.AIM To investigate the utility of plasma SHOX2,SEPTIN9,EPO,and RNF180 methylation in the clinical diagnosis and monitoring of EC.Plasma samples were collected from 210 patients at Hubei Cancer Hospital,and TaqMan polymerase chain reaction was employed to detect plasma SHOX2,SEPTIN9,RNF180,and EPO methylation.The area under the curve was used to estimate their diagnostic value for EC.Cox and logistic regression analyses were used to estimate the independent screening risk factors for patients with EC.RESULTS The sensitivity and specificity of combined assessment of plasma SHOX2,SEPTIN9,RNF180,and EPO methylation for adenocarcinoma,squamous cell carcinoma(SCC),and EC detection were 66.67%and 86.27%,77.40%and 85.29%,and 76.19%and 86.27%,respectively;the area under the curve values for diagnosing adenocarcinoma,SCC,and EC were 0.737[95%confidence interval(CI):0.584–0.89],0.824(95%CI:0.775–0.891),and 0.864(95%CI:0.809–0.92),respectively.CONCLUSION According to our findings,plasma SHOX2,SEPTIN9,RNF180,and EPO methylation exhibits appreciated sensitivity for diagnosing EC.The precise measurement of plasma SHOX2,SEPTIN9,RNF180,and EPO methylation can improve EC diagnosis and therapy efficacy monitoring.

Low-computation certificateless hybrid signcryption scheme

Hybrid signcryption is an important technique signcrypting bulk data using symmetric encryption. In this paper, we apply the technique of certificateless hybrid signcryption to an elliptic-curve cryptosystem, and construct a low-computation certificateless hybrid signcryption scheme. In the random oracle model, this scheme is proven to have indistinguishability against adaptive chosen-ciphertext attacks （IND-CCA2） under the elliptic-curve computation Diffie-Hellman assumption. Also, it has a strong existential unforgeability against adaptive chosen-message attacks （sUF-CMA） under the elliptic-curve discrete logarithm assumption. Analysis shows that the cryptographic algorithm does not rely on pairing operations and is much more etticient than other algorithms. In addition, it suits well to applications in environments where resources are constrained, such as wireless sensor networks and ad hoc networks.