The global coronavirus disease 2019(COVID-19)has become one of the biggest threats to the world since 2019.The respiratory and gastrointestinal tracts are the main targets for severe acute respiratory syndrome coronav...The global coronavirus disease 2019(COVID-19)has become one of the biggest threats to the world since 2019.The respiratory and gastrointestinal tracts are the main targets for severe acute respiratory syndrome coronavirus 2 infection for they highly express angiotensin-converting enzyme-2 and transmembrane protease serine 2.In patients suffering from COVID-19,gastrointestinal symptoms have ranged from 12%to 61%.Anorexia,nausea and/or vomiting,diarrhea,and abdominal pain are considered to be the main gastrointestinal symptoms of COVID-19.It has been reported that the direct damage of intestinal mucosal epithelial cells,malnutrition,and intestinal flora disorders are involved in COVID-19.However,the underlying mechanisms remain unclear.Thus,in this study,we reviewed and discussed the correlated mechanisms that cause gastrointestinal symptoms in order to help to develop the treatment strategy and build an appropriate guideline for medical workers.展开更多
Objective:This study aimed to investigate the differentiation of human adipose-derived stem cells(hASCs)into endometrial epithelial cells(EECs)under certain induction conditions and to a further step provide a promisi...Objective:This study aimed to investigate the differentiation of human adipose-derived stem cells(hASCs)into endometrial epithelial cells(EECs)under certain induction conditions and to a further step provide a promising approach for ASCs in clinical practice to the treatment of severe intrauterine adhesion.Methods:Four groups of hASCs were separately cultured as follows:in Group 1,hASCs were cultured in a control medium(5%fetal bovine serum[FBS]+α-minimum Eagle’s medium[α-MEM]);in Group 2,hASCs were cultured in an induction medium(5%FBS+α-MEM+[1×10-7 mol/L 17β-estradiol]+10 ng/mL transforming growth factorβ1[TGF-β1]+10 ng/mL epidermal growth factor[EGF]+10 ng/mL platelet-derived growth factor BB[PDGF-BB]);in Group 3,hASCs and human endometrium cells(hEMCs)were cocultured in the control medium;and in Group 4,hASCs and hEMCs were cocultured in the induction medium.Results:When cocultured with hEMCs,the morphology of hASCs became similar with EECs,and the addition of factors such as EGF,TGFβ,PDGF-BB,and 17β-estradiol promoted differentiation.This study,for the first time,demonstrated estrogen receptor(ER)αand ERβexpression in hASCs and preliminarily explored changes in ERα,ERβ,β-catenin,and H19 mRNA expression during hASC differentiation.Furthermore,we concluded that H19 mRNA expression was negatively correlated with differentiation,which is seemingly related to the estrogen signaling pathway.Conclusions:hASCs revealed the potential for differentiating to EECs when cocultured with hEMCs.展开更多
基金the Commission,No.KJZD-K202002701General Project of Chongqing Natural Science Foundation,No.cstc2019jcyj msxmX0299+3 种基金Key Project of Chongqing Three Gorges Medical College,No.XJ2021000101Chongqing Natural Drug Anti-Tumor Innovation Research Group,No.CXQT20030Special Fund for Key Laboratory of Development and Utilization of Genuine Medicinal Materials in Three Gorges Reservoir Area,No.Sys20210002the Open Project in 2022 of Chongqing Key Laboratory for the Development and Utilization of Authentic Medicinal Materials in the Three Gorges Reservoir Area,No.KFKT2022004.
文摘The global coronavirus disease 2019(COVID-19)has become one of the biggest threats to the world since 2019.The respiratory and gastrointestinal tracts are the main targets for severe acute respiratory syndrome coronavirus 2 infection for they highly express angiotensin-converting enzyme-2 and transmembrane protease serine 2.In patients suffering from COVID-19,gastrointestinal symptoms have ranged from 12%to 61%.Anorexia,nausea and/or vomiting,diarrhea,and abdominal pain are considered to be the main gastrointestinal symptoms of COVID-19.It has been reported that the direct damage of intestinal mucosal epithelial cells,malnutrition,and intestinal flora disorders are involved in COVID-19.However,the underlying mechanisms remain unclear.Thus,in this study,we reviewed and discussed the correlated mechanisms that cause gastrointestinal symptoms in order to help to develop the treatment strategy and build an appropriate guideline for medical workers.
基金funded by grants from the National Natural Science Foundation of China(No.81671463)the Key Research and Development Plan of Shaanxi Province(No.2017ZDCXL-SF-02-03)。
文摘Objective:This study aimed to investigate the differentiation of human adipose-derived stem cells(hASCs)into endometrial epithelial cells(EECs)under certain induction conditions and to a further step provide a promising approach for ASCs in clinical practice to the treatment of severe intrauterine adhesion.Methods:Four groups of hASCs were separately cultured as follows:in Group 1,hASCs were cultured in a control medium(5%fetal bovine serum[FBS]+α-minimum Eagle’s medium[α-MEM]);in Group 2,hASCs were cultured in an induction medium(5%FBS+α-MEM+[1×10-7 mol/L 17β-estradiol]+10 ng/mL transforming growth factorβ1[TGF-β1]+10 ng/mL epidermal growth factor[EGF]+10 ng/mL platelet-derived growth factor BB[PDGF-BB]);in Group 3,hASCs and human endometrium cells(hEMCs)were cocultured in the control medium;and in Group 4,hASCs and hEMCs were cocultured in the induction medium.Results:When cocultured with hEMCs,the morphology of hASCs became similar with EECs,and the addition of factors such as EGF,TGFβ,PDGF-BB,and 17β-estradiol promoted differentiation.This study,for the first time,demonstrated estrogen receptor(ER)αand ERβexpression in hASCs and preliminarily explored changes in ERα,ERβ,β-catenin,and H19 mRNA expression during hASC differentiation.Furthermore,we concluded that H19 mRNA expression was negatively correlated with differentiation,which is seemingly related to the estrogen signaling pathway.Conclusions:hASCs revealed the potential for differentiating to EECs when cocultured with hEMCs.
