Dear Editor,We reported a case of a multidisciplinary therapy for late recovered facial paralysis 2y after the initial diagnosis.Facial paralysis has an incidence of 20-30 people out of 100000 per year,and up to 1 in ...Dear Editor,We reported a case of a multidisciplinary therapy for late recovered facial paralysis 2y after the initial diagnosis.Facial paralysis has an incidence of 20-30 people out of 100000 per year,and up to 1 in 60 people will be affected during their life time[1].展开更多
The relationships between permeability and dynamics in hydrocarbon accumulation determine oil- bearing potential (the potential oil charge) of low perme- ability reservoirs. The evolution of porosity and permeabilit...The relationships between permeability and dynamics in hydrocarbon accumulation determine oil- bearing potential (the potential oil charge) of low perme- ability reservoirs. The evolution of porosity and permeability of low permeability turbidite reservoirs of the middle part of the third member of the Shahejie Formation in the Dongying Sag has been investigated by detailed core descriptions, thin section analyses, fluid inclusion analyses, carbon and oxygen isotope analyses, mercury injection, porosity and permeability testing, and basin modeling. The cutoff values for the permeability of the reservoirs in the accumulation period were calculated after detailing the accumulation dynamics and reservoir pore structures, then the distribution pattern of the oil-bearing potential of reservoirs controlled by the matching relationship between dynamics and permeability during the accumulation period were summarized. On the basis of the observed diagenetic features and with regard to the paragenetic sequences, the reservoirs can be subdivided into four types of diagenetic facies. The reservoirs experienced two periods of hydro- carbon accumulation. In the early accumulation period, the reservoirs except for diagenetic facies A had middle to high permeability ranging from 10 × 10-3 gm2 to 4207 × 10-3 lain2. In the later accumulation period, the reservoirs except for diagenetic facies C had low permeability ranging from 0.015 × 10-3 gm2 to 62× 10-3 -3m2. In the early accumulation period, the fluid pressure increased by the hydrocarbon generation was 1.4-11.3 MPa with an average value of 5.1 MPa, and a surplus pressure of 1.8-12.6 MPa with an average value of 6.3 MPa. In the later accumulation period, the fluid pressure increased by the hydrocarbon generation process was 0.7-12.7 MPa with an average value of 5.36 MPa and a surplus pressure of 1.3-16.2 MPa with an average value of 6.5 MPa. Even though different types of reservoirs exist, all can form hydrocarbon accumulations in the early accumulation per- iod. Such types of reservoirs can form hydrocarbon accumulation with high accumulation dynamics; however, reservoirs with diagenetic facies A and diagenetic facies B do not develop accumulation conditions with low accumu- lation dynamics in the late accumulation period for very low permeability. At more than 3000 m burial depth, a larger proportion of turbidite reservoirs are oil charged due to the proximity to the source rock, Also at these depths, lenticular sand bodies can accumulate hydrocarbons. At shallower depths, only the reservoirs with oil-source fault development can accumulate hydrocarbons. For flat surfaces, hydrocarbons have always been accumulated in the reservoirs around the oil-source faults and areas near the center of subsags with high accumulation dynamics.展开更多
Objective To study the role of sirtuin 1 (SIRT1) in Fas ligand (FasL) expression regulation during vascular lesion formation and to elucidate the potential mechanisms.Methods SIRT1 and FasL protein levels were detecte...Objective To study the role of sirtuin 1 (SIRT1) in Fas ligand (FasL) expression regulation during vascular lesion formation and to elucidate the potential mechanisms.Methods SIRT1 and FasL protein levels were detected by Western blotting in either mouse arteries extract or the whole rat aortic vascular smooth muscle cell (VSMC) lysate.Smooth muscle cell (SMC)-specific human SIRT1 transgenic (Tg) C57BL/6 mice and their littermate wild-type (WT) controls underwent complete carotid artery ligation (ligation groups) or the ligation-excluded operation (sham groups).The carotid arteries were collected 1 day after operation.Reverse transcription-polymerase chain reaction was performed to detect the mRNA levels of SIRT1 and FasL.Luciferase reporter assays were performed to detect the effect of WT-SIRT1,a dominant-negative form of SIRT1 (SIRT1H363Y),and GATA-6 on the promoter activity of FasL.Flow cytometry assay was applied to measure the hypodiploid DNA content of VSMC so as to monitor cellular apoptosis.Results SIRT1 was expressed in both rat aortic VSMCs and mouse arteries.Forced SIRT1 expression increased FasL expression both in injured mouse carotid arteries 1 day after ligation (P<0.001) and VSMCs treated with serum (P<0.05 at the transcriptional level,P<0.001 at the protein level).No notable apoptosis was observed.Furthermore,transcription factor GATA-6 increased the promoter activity of FasL (P<0.001).The induction of FasL promoter activity by GATA-6 was enhanced by WT-SIRT1 (P<0.001),while SIRT1H363Y significantly relieved the enhancing effect of WT-SIRT1 on GATA-6 (P<0.001).Conclusions Overexpression of SIRT1 up-regulates FasL expression in both flow-restricted mouse carotid arteries and serum-stimulated VSMCs.The transcription factor GATA-6 participates in the transcriptional regulation of FasL expression by SIRT1.展开更多
AIM:To investigate the clinical characteristics and prognosis of patients with malignant eyelid tumors.·METHODS:This was a retrospective,non-randomized,clinical reviews.Between January,2002 and December,2011,75 c...AIM:To investigate the clinical characteristics and prognosis of patients with malignant eyelid tumors.·METHODS:This was a retrospective,non-randomized,clinical reviews.Between January,2002 and December,2011,75 cases with histologically confirmed malignant eyelid tumors were evaluated.Patients’ charts were reviewed for clinical information,treatment procedure,and disease course.Survival analysis in terms of recurrence-free survival was performed using age,sex,location of tumor and histopathological type.The followup ranged from 1 to 78 months(mean=21 months).·RESULTS:The 75 eyelid tumors included 35 basal cell carcinoma(BCC,46.7%),22 sebaceous gland carcinoma(SGC,29.3%),7 squamous cell carcinoma(SCC,9.3%),10 malignant melanoma(MM,13.3%),and 1 Merkel cell carcinoma(MCC,1.3%).Recurrence developed in 17cases(22.7%).The recurrence rate of BCC(4/35,11.4%)was significant lower than MM(6/10,60.0%,P 【0.001).The mean interval of recurrence was 21 months(range 3-62) for all eyelid tumors.Tumor located at canthus had higher recurrence rate(50%) compared with those located at eyelid(19%,P 【0.05).Histological type was independent variable for recurrence by Cox regression analysis.·CONCLUSION:It is important to achieve a negative tumor margin in canthus located malignant eyelid tumor.Clinicians should have a high level of suspicion for recurrence according to histological type when treating patients with eyelid tumor.展开更多
基金Supported by the National Natural Science Foundation of China (No.82000938,No.81900906,No.82000857)。
文摘Dear Editor,We reported a case of a multidisciplinary therapy for late recovered facial paralysis 2y after the initial diagnosis.Facial paralysis has an incidence of 20-30 people out of 100000 per year,and up to 1 in 60 people will be affected during their life time[1].
基金supported by the National Natural Science Foundation of China(Grant No.U1262203)the National Science and Technology Special Grant(No.2011ZX05006-003)+1 种基金the Fundamental Research Funds for the Central Universities(Grant No.14CX06070A)the Chinese Scholarship Council(No.201506450029)
文摘The relationships between permeability and dynamics in hydrocarbon accumulation determine oil- bearing potential (the potential oil charge) of low perme- ability reservoirs. The evolution of porosity and permeability of low permeability turbidite reservoirs of the middle part of the third member of the Shahejie Formation in the Dongying Sag has been investigated by detailed core descriptions, thin section analyses, fluid inclusion analyses, carbon and oxygen isotope analyses, mercury injection, porosity and permeability testing, and basin modeling. The cutoff values for the permeability of the reservoirs in the accumulation period were calculated after detailing the accumulation dynamics and reservoir pore structures, then the distribution pattern of the oil-bearing potential of reservoirs controlled by the matching relationship between dynamics and permeability during the accumulation period were summarized. On the basis of the observed diagenetic features and with regard to the paragenetic sequences, the reservoirs can be subdivided into four types of diagenetic facies. The reservoirs experienced two periods of hydro- carbon accumulation. In the early accumulation period, the reservoirs except for diagenetic facies A had middle to high permeability ranging from 10 × 10-3 gm2 to 4207 × 10-3 lain2. In the later accumulation period, the reservoirs except for diagenetic facies C had low permeability ranging from 0.015 × 10-3 gm2 to 62× 10-3 -3m2. In the early accumulation period, the fluid pressure increased by the hydrocarbon generation was 1.4-11.3 MPa with an average value of 5.1 MPa, and a surplus pressure of 1.8-12.6 MPa with an average value of 6.3 MPa. In the later accumulation period, the fluid pressure increased by the hydrocarbon generation process was 0.7-12.7 MPa with an average value of 5.36 MPa and a surplus pressure of 1.3-16.2 MPa with an average value of 6.5 MPa. Even though different types of reservoirs exist, all can form hydrocarbon accumulations in the early accumulation per- iod. Such types of reservoirs can form hydrocarbon accumulation with high accumulation dynamics; however, reservoirs with diagenetic facies A and diagenetic facies B do not develop accumulation conditions with low accumu- lation dynamics in the late accumulation period for very low permeability. At more than 3000 m burial depth, a larger proportion of turbidite reservoirs are oil charged due to the proximity to the source rock, Also at these depths, lenticular sand bodies can accumulate hydrocarbons. At shallower depths, only the reservoirs with oil-source fault development can accumulate hydrocarbons. For flat surfaces, hydrocarbons have always been accumulated in the reservoirs around the oil-source faults and areas near the center of subsags with high accumulation dynamics.
基金Supported by the National Natural Science Foundation of China(81102444)Special Fund of the National Laboratory of China(2060204)
文摘Objective To study the role of sirtuin 1 (SIRT1) in Fas ligand (FasL) expression regulation during vascular lesion formation and to elucidate the potential mechanisms.Methods SIRT1 and FasL protein levels were detected by Western blotting in either mouse arteries extract or the whole rat aortic vascular smooth muscle cell (VSMC) lysate.Smooth muscle cell (SMC)-specific human SIRT1 transgenic (Tg) C57BL/6 mice and their littermate wild-type (WT) controls underwent complete carotid artery ligation (ligation groups) or the ligation-excluded operation (sham groups).The carotid arteries were collected 1 day after operation.Reverse transcription-polymerase chain reaction was performed to detect the mRNA levels of SIRT1 and FasL.Luciferase reporter assays were performed to detect the effect of WT-SIRT1,a dominant-negative form of SIRT1 (SIRT1H363Y),and GATA-6 on the promoter activity of FasL.Flow cytometry assay was applied to measure the hypodiploid DNA content of VSMC so as to monitor cellular apoptosis.Results SIRT1 was expressed in both rat aortic VSMCs and mouse arteries.Forced SIRT1 expression increased FasL expression both in injured mouse carotid arteries 1 day after ligation (P<0.001) and VSMCs treated with serum (P<0.05 at the transcriptional level,P<0.001 at the protein level).No notable apoptosis was observed.Furthermore,transcription factor GATA-6 increased the promoter activity of FasL (P<0.001).The induction of FasL promoter activity by GATA-6 was enhanced by WT-SIRT1 (P<0.001),while SIRT1H363Y significantly relieved the enhancing effect of WT-SIRT1 on GATA-6 (P<0.001).Conclusions Overexpression of SIRT1 up-regulates FasL expression in both flow-restricted mouse carotid arteries and serum-stimulated VSMCs.The transcription factor GATA-6 participates in the transcriptional regulation of FasL expression by SIRT1.
基金National Natural Science Foundation of China (No.81070756)National "Twelfth Five-Year" Plan for Science and Technology Support Program (No.2012BAI08B01)+1 种基金Zhejiang Key Innovation Team Project of China (No.2009R50039)Zhejiang Key Laboratory Fund of China (No.2011E10006)
文摘AIM:To investigate the clinical characteristics and prognosis of patients with malignant eyelid tumors.·METHODS:This was a retrospective,non-randomized,clinical reviews.Between January,2002 and December,2011,75 cases with histologically confirmed malignant eyelid tumors were evaluated.Patients’ charts were reviewed for clinical information,treatment procedure,and disease course.Survival analysis in terms of recurrence-free survival was performed using age,sex,location of tumor and histopathological type.The followup ranged from 1 to 78 months(mean=21 months).·RESULTS:The 75 eyelid tumors included 35 basal cell carcinoma(BCC,46.7%),22 sebaceous gland carcinoma(SGC,29.3%),7 squamous cell carcinoma(SCC,9.3%),10 malignant melanoma(MM,13.3%),and 1 Merkel cell carcinoma(MCC,1.3%).Recurrence developed in 17cases(22.7%).The recurrence rate of BCC(4/35,11.4%)was significant lower than MM(6/10,60.0%,P 【0.001).The mean interval of recurrence was 21 months(range 3-62) for all eyelid tumors.Tumor located at canthus had higher recurrence rate(50%) compared with those located at eyelid(19%,P 【0.05).Histological type was independent variable for recurrence by Cox regression analysis.·CONCLUSION:It is important to achieve a negative tumor margin in canthus located malignant eyelid tumor.Clinicians should have a high level of suspicion for recurrence according to histological type when treating patients with eyelid tumor.