Human thioredoxin reductase (TrxR) system is associated with cancer cell growth and anti-apoptosis process. Effects of 1,2-bis(1,2-benzisoselenazolone-3(2H)-ketone)ethane (BBSKE),a novel TrxR inhibitor,were investigat...Human thioredoxin reductase (TrxR) system is associated with cancer cell growth and anti-apoptosis process. Effects of 1,2-bis(1,2-benzisoselenazolone-3(2H)-ketone)ethane (BBSKE),a novel TrxR inhibitor,were investigated on human leu-kemia cell lines HL-60 and K562. BBSKE treatment induced cell growth inhibition and apoptosis in both cell lines. Apoptosis induced by BBSKE is through Bcl-2/Bax and caspase-3 pathways. Ehrlich's ascites carcinoma-bearing mice were used to inves-tigate the anti-tumor effect of BBSKE in vivo. Tumor-bearing mice treated with BBSKE showed an increase of life span with a comparable effect to cyclophosphamide (CTX). These results suggest a potential usage of BBSKE as a therapeutic agent against non-solid tumors.展开更多
BACKGROUND Gastric cancer is a leading cause of cancer-related mortality worldwide.Many somatic mutations have been identified based on next-generation sequencing;they likely play a vital role in cancer treatment sele...BACKGROUND Gastric cancer is a leading cause of cancer-related mortality worldwide.Many somatic mutations have been identified based on next-generation sequencing;they likely play a vital role in cancer treatment selection.However,nextgeneration sequencing has not been widely used to diagnose and treat gastric cancer in the clinic.AIM To test the mutant gene frequency as a guide for molecular diagnosis and personalized therapy in gastric cancer by use of next-generation sequencing.METHODS We constructed a panel of 24 mutant genes to detect somatic nucleotide variations and copy number variations based on a next-generation sequencing technique.Our custom panel included high-mutation frequency cancer driver and tumour suppressor genes.Mutated genes were also analyzed using the cBioPortal database.The clinical annotation of important variant mutation sites was evaluated in the ClinVar database.We searched for candidate drugs for targeted therapy and immunotherapy from the OncoKB database.RESULTS In our study,the top 16 frequently mutated genes were TP53(58%),ERBB2(28%),BRCA2(23%),NF1(19%),PIK3CA(14%),ATR(14%),MSH2(12%),FBXW7(12%),BMPR1A(12%),ERBB3(11%),ATM(9%),FGFR2(8%),MET(8%),PTEN(6%),CHD4(6%),and KRAS(5%).TP53 is a commonly mutated gene in gastric cancer and has a similar frequency to that in the cBioPortal database.33 gastric cancer patients(51.6%)with microsatellite stability and eight patients(12.5%)with microsatellite instability-high were investigated.Enrichment analyses demonstrated that high-frequency mutated genes had transmembrane receptor protein kinase activity.We discovered that BRCA2,PIK3CA,and FGFR2 gene mutations represent promising biomarkers in gastric cancer.CONCLUSION We developed a powerful panel of 24 genes with high frequencies of mutation that could detect common somatic mutations.The observed mutations provide potential targets for the clinical treatment of gastric cancer.展开更多
Chronic obstructive pulmonary disease(COPD)is a respiratory disease with a high incidence,mortality,and disability rate.Because there are few symptoms in the early stages of COPD,diagnosis and treatment are seriously ...Chronic obstructive pulmonary disease(COPD)is a respiratory disease with a high incidence,mortality,and disability rate.Because there are few symptoms in the early stages of COPD,diagnosis and treatment are seriously insufficient.It is necessary to find effective clues for early COPD diagnosis and provide appropriate interventions.Several studies suggest that small airway disease is the earliest stage of COPD because it is correlated with subsequent development of airflow obstruction.However,there are currently no globally accepted criteria for defining early COPD.This study mainly introduced risk factors,definition,diagnosis,and treatment of early COPD from a new perspective.展开更多
The coronavirus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) has posed severe threats to human health,public safety,and the global economy.Metal nutrient elements can dir...The coronavirus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) has posed severe threats to human health,public safety,and the global economy.Metal nutrient elements can directly or indirectly take part in human immune responses,and metal-related drugs have served as antiviral drugs and/or enzyme inhibitors for many years,providing potential solutions to the prevention and treatment of COVID-19.Metal-based drugs are currently under a variety of chemical structures and exhibit wide-range bio activities,demonstrating irreplaceable advantages in pharmacology.This review is an intention to summarize recent progress in the prevention and treatment strategies against COVID-19 from the perspective of metal pharmacology.The current and potential utilization of metal-based drugs is briefly introduced.Specifically,metallohydrogels that have been shown to present superior antiviral activities are stressed in the paper as potential drugs for the treatment of COVID-19.展开更多
基金Project (No.30472036) supported by the National Natural Science Foundation of China
文摘Human thioredoxin reductase (TrxR) system is associated with cancer cell growth and anti-apoptosis process. Effects of 1,2-bis(1,2-benzisoselenazolone-3(2H)-ketone)ethane (BBSKE),a novel TrxR inhibitor,were investigated on human leu-kemia cell lines HL-60 and K562. BBSKE treatment induced cell growth inhibition and apoptosis in both cell lines. Apoptosis induced by BBSKE is through Bcl-2/Bax and caspase-3 pathways. Ehrlich's ascites carcinoma-bearing mice were used to inves-tigate the anti-tumor effect of BBSKE in vivo. Tumor-bearing mice treated with BBSKE showed an increase of life span with a comparable effect to cyclophosphamide (CTX). These results suggest a potential usage of BBSKE as a therapeutic agent against non-solid tumors.
基金Supported by the Science and Technology Foundation of Guangzhou,No.201803010059the Natural Science Foundation of Bengbu Medical College,No.BYKY2019129ZD.
文摘BACKGROUND Gastric cancer is a leading cause of cancer-related mortality worldwide.Many somatic mutations have been identified based on next-generation sequencing;they likely play a vital role in cancer treatment selection.However,nextgeneration sequencing has not been widely used to diagnose and treat gastric cancer in the clinic.AIM To test the mutant gene frequency as a guide for molecular diagnosis and personalized therapy in gastric cancer by use of next-generation sequencing.METHODS We constructed a panel of 24 mutant genes to detect somatic nucleotide variations and copy number variations based on a next-generation sequencing technique.Our custom panel included high-mutation frequency cancer driver and tumour suppressor genes.Mutated genes were also analyzed using the cBioPortal database.The clinical annotation of important variant mutation sites was evaluated in the ClinVar database.We searched for candidate drugs for targeted therapy and immunotherapy from the OncoKB database.RESULTS In our study,the top 16 frequently mutated genes were TP53(58%),ERBB2(28%),BRCA2(23%),NF1(19%),PIK3CA(14%),ATR(14%),MSH2(12%),FBXW7(12%),BMPR1A(12%),ERBB3(11%),ATM(9%),FGFR2(8%),MET(8%),PTEN(6%),CHD4(6%),and KRAS(5%).TP53 is a commonly mutated gene in gastric cancer and has a similar frequency to that in the cBioPortal database.33 gastric cancer patients(51.6%)with microsatellite stability and eight patients(12.5%)with microsatellite instability-high were investigated.Enrichment analyses demonstrated that high-frequency mutated genes had transmembrane receptor protein kinase activity.We discovered that BRCA2,PIK3CA,and FGFR2 gene mutations represent promising biomarkers in gastric cancer.CONCLUSION We developed a powerful panel of 24 genes with high frequencies of mutation that could detect common somatic mutations.The observed mutations provide potential targets for the clinical treatment of gastric cancer.
基金the National Science Foundation of China(No.81270100,No.81600031)the National Key R&D Program of China(2016YFC1304700).
文摘Chronic obstructive pulmonary disease(COPD)is a respiratory disease with a high incidence,mortality,and disability rate.Because there are few symptoms in the early stages of COPD,diagnosis and treatment are seriously insufficient.It is necessary to find effective clues for early COPD diagnosis and provide appropriate interventions.Several studies suggest that small airway disease is the earliest stage of COPD because it is correlated with subsequent development of airflow obstruction.However,there are currently no globally accepted criteria for defining early COPD.This study mainly introduced risk factors,definition,diagnosis,and treatment of early COPD from a new perspective.
基金supported in part by the National Natural Science Foundation of China(No.81372266)the National Science and Technology Major Project of the Ministry of Science and Technology of China(No.2011zx09101-001-03)
基金Project supported by the National Natural Science Foundation of China(No.81372266)the National Science and Technology Major Project(No.2011zx09101-001-03)China
基金Hunan Provincial Key Laboratory of Micro&Nano Materials Interface Sciencethe National Natural Science Foundation of China(Nos.21773311 and 21972169)Hunan Provincial Science and Technology Plan Project,China(No.2019TP1001)。
文摘The coronavirus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) has posed severe threats to human health,public safety,and the global economy.Metal nutrient elements can directly or indirectly take part in human immune responses,and metal-related drugs have served as antiviral drugs and/or enzyme inhibitors for many years,providing potential solutions to the prevention and treatment of COVID-19.Metal-based drugs are currently under a variety of chemical structures and exhibit wide-range bio activities,demonstrating irreplaceable advantages in pharmacology.This review is an intention to summarize recent progress in the prevention and treatment strategies against COVID-19 from the perspective of metal pharmacology.The current and potential utilization of metal-based drugs is briefly introduced.Specifically,metallohydrogels that have been shown to present superior antiviral activities are stressed in the paper as potential drugs for the treatment of COVID-19.
