With the acceleration of population aging,heart disease has been a high priority,and cardiac ion channel research has been one of the hot spots in the field,Sodium ion channels,as the most important class,have been wi...With the acceleration of population aging,heart disease has been a high priority,and cardiac ion channel research has been one of the hot spots in the field,Sodium ion channels,as the most important class,have been widely concerned.Therefore,this paper briefly introduces and discusses three aspects of the structure and function of sodium ion channels and the development of drug research.展开更多
Objective: This r esea rch showed a method of isolate rat cardio myocytes though acute enzymatic hydrolysis. Method: Our method was divided into five steps: preparation, heart ext raction, perfusion, mechanical dissoc...Objective: This r esea rch showed a method of isolate rat cardio myocytes though acute enzymatic hydrolysis. Method: Our method was divided into five steps: preparation, heart ext raction, perfusion, mechanical dissociation and pu rifica tion and r ecalcifica ti on. Fir stly, make a preparation, including solutions, equipment and so on. Secondly, after anesthesia satisfactorily, open the chest of rat and take the heart out. Thirdly, transect the aorta and hang the aorta onto the Langendroff perfusion system such that the tip is just distal to the aortic valve. For th, perfuse the hea rt with diges tion solution for 17-20 min afte r Ca2+ free tyrode solution for 5 min. Finally, remove the heart from the system quickly and dissociate mechanically in the KB solution and recalcification after filter. Result: Though the microscope, good-state cardiomyocytes (clear stripes, strong sense of three-dimensional, stick well) can be observed. Conclusion: Those cells can be used to undergo medical electrophysiological experiment, including the study of iron channel.展开更多
Sodium ion channels are the main activation of electrical signals,which are the basis of a series of physiological processes such as nerve activity and muscle contraction of excitable cells.Traditional Chinese medicin...Sodium ion channels are the main activation of electrical signals,which are the basis of a series of physiological processes such as nerve activity and muscle contraction of excitable cells.Traditional Chinese medicine(TCM)has a history of more than 2000 years,and there are more and more researches about TCM on sodium ion channels.This article summarized these researches and had a prospect on its future as well as the next possible focuses in order to lay a foundation for the modern pharmacology and development and promotion of TCM.展开更多
Objective:To report a patient with acne by using the combined treatment of pricking cupping bloodletting and slide cupping.Methods:Pricking cupping bloodletting was prescribed at the points of Feishu(BL13)in the bladd...Objective:To report a patient with acne by using the combined treatment of pricking cupping bloodletting and slide cupping.Methods:Pricking cupping bloodletting was prescribed at the points of Feishu(BL13)in the bladder meridian of foot-Taiyang and Dazhui(DU14)in governor vessel and slide cupping was prescribed at the points on the back in the bladder meridian of foot-Taiyang.Results:Clinical manifestations revealed that acne and scar were significantly reduced after two courses.Conclusion:Pricking cupping bloodletting combined with slide cupping can successfully relieve acne and promote skin healing,which may provide us a new idea for the non-drug therapy of acne.展开更多
文摘With the acceleration of population aging,heart disease has been a high priority,and cardiac ion channel research has been one of the hot spots in the field,Sodium ion channels,as the most important class,have been widely concerned.Therefore,this paper briefly introduces and discusses three aspects of the structure and function of sodium ion channels and the development of drug research.
文摘Objective: This r esea rch showed a method of isolate rat cardio myocytes though acute enzymatic hydrolysis. Method: Our method was divided into five steps: preparation, heart ext raction, perfusion, mechanical dissociation and pu rifica tion and r ecalcifica ti on. Fir stly, make a preparation, including solutions, equipment and so on. Secondly, after anesthesia satisfactorily, open the chest of rat and take the heart out. Thirdly, transect the aorta and hang the aorta onto the Langendroff perfusion system such that the tip is just distal to the aortic valve. For th, perfuse the hea rt with diges tion solution for 17-20 min afte r Ca2+ free tyrode solution for 5 min. Finally, remove the heart from the system quickly and dissociate mechanically in the KB solution and recalcification after filter. Result: Though the microscope, good-state cardiomyocytes (clear stripes, strong sense of three-dimensional, stick well) can be observed. Conclusion: Those cells can be used to undergo medical electrophysiological experiment, including the study of iron channel.
文摘Sodium ion channels are the main activation of electrical signals,which are the basis of a series of physiological processes such as nerve activity and muscle contraction of excitable cells.Traditional Chinese medicine(TCM)has a history of more than 2000 years,and there are more and more researches about TCM on sodium ion channels.This article summarized these researches and had a prospect on its future as well as the next possible focuses in order to lay a foundation for the modern pharmacology and development and promotion of TCM.
文摘Objective:To report a patient with acne by using the combined treatment of pricking cupping bloodletting and slide cupping.Methods:Pricking cupping bloodletting was prescribed at the points of Feishu(BL13)in the bladder meridian of foot-Taiyang and Dazhui(DU14)in governor vessel and slide cupping was prescribed at the points on the back in the bladder meridian of foot-Taiyang.Results:Clinical manifestations revealed that acne and scar were significantly reduced after two courses.Conclusion:Pricking cupping bloodletting combined with slide cupping can successfully relieve acne and promote skin healing,which may provide us a new idea for the non-drug therapy of acne.
