L-arabinose isomerase (L-AI) is the key enzyme for D-galactose isomerization of D-tagatose by biological method. In this research, Lactobacillus plantarum WU14 with high D-tagatose yield was identified as Lactobacillu...L-arabinose isomerase (L-AI) is the key enzyme for D-galactose isomerization of D-tagatose by biological method. In this research, Lactobacillus plantarum WU14 with high D-tagatose yield was identified as Lactobacillus plantarum was isolated from the number of lactic acid bacteria from pickled vegetables. The crude L-arabinose isomerase activity of Lactobacillus plantarum WU14 with high D-tagatose yield was 13.95 U/mL under the optimal temperature 60°C, pH 7.17 and substrate concentration 0.8 mol/L, and the conversion rate of 56.12% could be gained after 28 hours. Protein structure and specific of L-Arabinose Isomerase of Lactobacillus plantarum WU14 were researched. The results showed that L-arabinose isomerase is mainly composed of alpha helix and random coil. Then the recombinant L-AI gene was inserted into the food-grade expression vector pRNA48 and expressed in L. lactis NZ9000 successfully. The target protein expression reached the maximum amount when the induced concentration of nisin reaches 30 ng/mL after 12 h. And the crude enzyme activity of recombinant bacteria reached 6.21 U/mL under 60°C. Otherwise the optimal conversion rate recombinant of L. lactis NZ9000/pRNA48-L-AI can reach 39.21% under the temperature of 50°C, pH 7.17 and D-galactose concentration was 0.6 mol/L.展开更多
Objective:To explore the effect ofhospital nursing management based on informatization.Methods:167 inpatients from different departments in our hospital from June 2020 to November 2019 were randomly selected and divid...Objective:To explore the effect ofhospital nursing management based on informatization.Methods:167 inpatients from different departments in our hospital from June 2020 to November 2019 were randomly selected and divided into control group and observation group.83 cases in the control group were treated with traditional nursing management mode;87 cases in the observation group used modem nursing information management system for information-based nursing management,and then compared the two groups in terms of nursing management efficiency,patient basic nursing,ward management,specialized nursing and nursing satisfaction,so as to verify the clinical application effect of information-based nursing management.Results:After the application of information-based nursing management mode,the construction and application of information management system made the nursing management process more standardized and accurate.Therefore,the clinical nursing efficiency,basic nursing,ward management,specialized nursing and nursing satisfaction of the observation group were better than those of the control group.Conclusion:In the practice ofhospital inpatient nursing management,the scientific use of information technology and information management system can significantly improve the efficiency of patient nursing management and nursing satisfaction,and play a key role in improving the prognosis of patients.Therefore,it is worth popularizing and applying in the whole hospital and even the national grass-roots hospitals.展开更多
文摘L-arabinose isomerase (L-AI) is the key enzyme for D-galactose isomerization of D-tagatose by biological method. In this research, Lactobacillus plantarum WU14 with high D-tagatose yield was identified as Lactobacillus plantarum was isolated from the number of lactic acid bacteria from pickled vegetables. The crude L-arabinose isomerase activity of Lactobacillus plantarum WU14 with high D-tagatose yield was 13.95 U/mL under the optimal temperature 60°C, pH 7.17 and substrate concentration 0.8 mol/L, and the conversion rate of 56.12% could be gained after 28 hours. Protein structure and specific of L-Arabinose Isomerase of Lactobacillus plantarum WU14 were researched. The results showed that L-arabinose isomerase is mainly composed of alpha helix and random coil. Then the recombinant L-AI gene was inserted into the food-grade expression vector pRNA48 and expressed in L. lactis NZ9000 successfully. The target protein expression reached the maximum amount when the induced concentration of nisin reaches 30 ng/mL after 12 h. And the crude enzyme activity of recombinant bacteria reached 6.21 U/mL under 60°C. Otherwise the optimal conversion rate recombinant of L. lactis NZ9000/pRNA48-L-AI can reach 39.21% under the temperature of 50°C, pH 7.17 and D-galactose concentration was 0.6 mol/L.
文摘Objective:To explore the effect ofhospital nursing management based on informatization.Methods:167 inpatients from different departments in our hospital from June 2020 to November 2019 were randomly selected and divided into control group and observation group.83 cases in the control group were treated with traditional nursing management mode;87 cases in the observation group used modem nursing information management system for information-based nursing management,and then compared the two groups in terms of nursing management efficiency,patient basic nursing,ward management,specialized nursing and nursing satisfaction,so as to verify the clinical application effect of information-based nursing management.Results:After the application of information-based nursing management mode,the construction and application of information management system made the nursing management process more standardized and accurate.Therefore,the clinical nursing efficiency,basic nursing,ward management,specialized nursing and nursing satisfaction of the observation group were better than those of the control group.Conclusion:In the practice ofhospital inpatient nursing management,the scientific use of information technology and information management system can significantly improve the efficiency of patient nursing management and nursing satisfaction,and play a key role in improving the prognosis of patients.Therefore,it is worth popularizing and applying in the whole hospital and even the national grass-roots hospitals.
