Many genetic loci for wheat plant height(PH) have been reported, and 26 dwarfing genes have been catalogued. To identify major and stable genetic loci for PH, here we thoroughly summarized these functionally or geneti...Many genetic loci for wheat plant height(PH) have been reported, and 26 dwarfing genes have been catalogued. To identify major and stable genetic loci for PH, here we thoroughly summarized these functionally or genetic verified dwarfing loci from QTL linkage analysis and genome-wide association study published from 2003 to 2022. A total of 332 QTL, 270 GWAS loci and 83 genes for PH were integrated onto chromosomes according to their locations in the IWGSC RefSeq v2.1 and 65 QTL-rich clusters(QRC) were defined. Candidate genes in each QRC were predicted based on IWGSC Annotation v2.1 and the information on functional validation of homologous genes in other species. A total of 38 candidate genes were predicted for 65 QRC including three GA2ox genes in QRC-4B-IV, QRC-5A-VIII and QRC-6A-II(Rht24) as well as GA 20-oxidase 2(TaSD1-3A) in QRC-3A-IV. These outcomes lay concrete foundations for mapbased cloning of wheat dwarfing genes and application in breeding.展开更多
Bacterial infections exacerbate the formation of bacterial biofilms,leading to resistance to traditional drugs,persistent infection,and even threatening patient’s life.Efficient antimicrobial materials against drug-r...Bacterial infections exacerbate the formation of bacterial biofilms,leading to resistance to traditional drugs,persistent infection,and even threatening patient’s life.Efficient antimicrobial materials against drug-resistant bacterial biofilms are highly desired.In this study,a photodynamic nanodrug with bacterial targeting was constructed by cooperative coordination of zinc ion with an antimicrobial peptide with hydrophobic tripeptides on the side chains and the photosensitizer chlorin e6.The supramolecular nanodrug with a uniform spherical structure possessed high photosensitizer loading capacity and enhanced photodynamic efficacy,which could deep penetrate and eradicate methicillin-resistant Staphylococcus aureus(MRSA)biofilms upon 655 nm laser irradiation.Furthermore,in vivo experiments verified the efficient elimination of MRSA biofilms on implanted catheters.This study provides a novel strategy to fabricate metalloprotein-inspired supramolecular photodynamic nanodrugs against drugresistant bacterial biofilms-associated infections in vivo.展开更多
Recently,in-situ polymerization inside living cells has attracted much attention due to the efficient cellular internalization and elevated drug retention.However,the lack of tracking of the in-situ polymerization pro...Recently,in-situ polymerization inside living cells has attracted much attention due to the efficient cellular internalization and elevated drug retention.However,the lack of tracking of the in-situ polymerization process and the unclear effects of polymerization on cellular functions restrict its biomedical applications.Herein,we designed a Y-shaped diacetylene-containing lipidated peptide amphiphile(YDLPA1)with positive charges,which underwent in-situ polymerization initiated by reactive oxygen species in the intracellular microenvironment.In comparison,zwitterionic YDLPA2 and negatively charged Y-DLPA3 were polymerized in aqueous solution,but cannot polymerize in the intracellular microenvironment.The polymerized Y-DLPA1 with red fluorescence provides a platform to label cells for long-term tracking studies.This polymerization reaction induced tumor cell apoptosis,increased cell viscosity and decreased cell motility,which potentially inhibited tumor metastasis and served as a novel antitumor agent.This work provides a novel strategy to track in-situ polymerization process and modulate cell biofunctions.展开更多
基金funded by the National Natural Science Foundation of China (32101733)Shandong Provincial Natural Science Foundation (ZR202103020229)+1 种基金the High-Level Talents Project of Qingdao Agricultural University (663/1122023)National Natural Science Foundation of China Regional Innovation and Development Joint Fund Project (U22A20457)。
文摘Many genetic loci for wheat plant height(PH) have been reported, and 26 dwarfing genes have been catalogued. To identify major and stable genetic loci for PH, here we thoroughly summarized these functionally or genetic verified dwarfing loci from QTL linkage analysis and genome-wide association study published from 2003 to 2022. A total of 332 QTL, 270 GWAS loci and 83 genes for PH were integrated onto chromosomes according to their locations in the IWGSC RefSeq v2.1 and 65 QTL-rich clusters(QRC) were defined. Candidate genes in each QRC were predicted based on IWGSC Annotation v2.1 and the information on functional validation of homologous genes in other species. A total of 38 candidate genes were predicted for 65 QRC including three GA2ox genes in QRC-4B-IV, QRC-5A-VIII and QRC-6A-II(Rht24) as well as GA 20-oxidase 2(TaSD1-3A) in QRC-3A-IV. These outcomes lay concrete foundations for mapbased cloning of wheat dwarfing genes and application in breeding.
基金the National Natural Science Foundation of China(No.52173124)the Fundamental Research Funds for the Central Universities(No.2172019kfyXJJS070).
文摘Bacterial infections exacerbate the formation of bacterial biofilms,leading to resistance to traditional drugs,persistent infection,and even threatening patient’s life.Efficient antimicrobial materials against drug-resistant bacterial biofilms are highly desired.In this study,a photodynamic nanodrug with bacterial targeting was constructed by cooperative coordination of zinc ion with an antimicrobial peptide with hydrophobic tripeptides on the side chains and the photosensitizer chlorin e6.The supramolecular nanodrug with a uniform spherical structure possessed high photosensitizer loading capacity and enhanced photodynamic efficacy,which could deep penetrate and eradicate methicillin-resistant Staphylococcus aureus(MRSA)biofilms upon 655 nm laser irradiation.Furthermore,in vivo experiments verified the efficient elimination of MRSA biofilms on implanted catheters.This study provides a novel strategy to fabricate metalloprotein-inspired supramolecular photodynamic nanodrugs against drugresistant bacterial biofilms-associated infections in vivo.
基金supported by the National Natural Science Foundation of China(52173124)the Fundamental Research Funds for the Central Universities(2172019kfyXJJS070)。
文摘Recently,in-situ polymerization inside living cells has attracted much attention due to the efficient cellular internalization and elevated drug retention.However,the lack of tracking of the in-situ polymerization process and the unclear effects of polymerization on cellular functions restrict its biomedical applications.Herein,we designed a Y-shaped diacetylene-containing lipidated peptide amphiphile(YDLPA1)with positive charges,which underwent in-situ polymerization initiated by reactive oxygen species in the intracellular microenvironment.In comparison,zwitterionic YDLPA2 and negatively charged Y-DLPA3 were polymerized in aqueous solution,but cannot polymerize in the intracellular microenvironment.The polymerized Y-DLPA1 with red fluorescence provides a platform to label cells for long-term tracking studies.This polymerization reaction induced tumor cell apoptosis,increased cell viscosity and decreased cell motility,which potentially inhibited tumor metastasis and served as a novel antitumor agent.This work provides a novel strategy to track in-situ polymerization process and modulate cell biofunctions.
