Phase measuring deflectometry(PMD)is a robust,noncoherent technique for the characterization of specular surface.For measuring high specular reflectivity surface,PMD can deliver micron radian range local gradient.Howe...Phase measuring deflectometry(PMD)is a robust,noncoherent technique for the characterization of specular surface.For measuring high specular reflectivity surface,PMD can deliver micron radian range local gradient.However,when the measured surface has low specular reflectivity,the accuracy of the measured gradient is low since the captured fringe pattern shows low signal to noise ratio.The phase error characteristics in PMD system when testing low reflectivity surfaces are analyzed.The analysis illustrates that the random phase error increases rapidly while the nonlinear error drops slowly with the decreasing of the tested surface reflectivity.In order to attain high precision measurement of low reflectivity specular surface,a robust error reduction method based on wavelet de-noising is proposed to reduce the phase error.This error reduction method is compared with several other normally used methods in both simulation and experiment work.The method based on the wavelet de-noising shows better performance when measuring the low reflectivity specular surface.展开更多
A hallmark of systemic lupus erythematosus （SLE） is the consistent production of various auto-antibodies by auto-reactive B cells. Interferon-α（IFN-α） signaling is highly activated in SLE B cells and plays a vit...A hallmark of systemic lupus erythematosus （SLE） is the consistent production of various auto-antibodies by auto-reactive B cells. Interferon-α（IFN-α） signaling is highly activated in SLE B cells and plays a vital role in the antibody response by B cells. Previous studies have shown that CD180-negative B cells, which are dramatically increased in SLE patients, are responsible for the production of auto-antibodies. However, the association between CD180 and IFN-αsignaling remains unknown. In the present study, we explored the effect of CD180 on regulating the activation of IFN-α signaling in B cells. We found that the number of CD180-negative B cells was increased in MRIJMp-Fas（Ipr/Ipr） lupus-prone mice compared with wild-type mice. Phenotypic analysis showed that CD180-negative B cells comprised CD138＋ plasmablast/plasma cells and GL-7＋ germinal center （GC） B cells. Notably, ligation of CD180 significantly inhibited the I FN-α-induced phosphorylation of signal transducer and activator of transcription 2 （STAT-2） and expression of IFN-stimulated genes （ISGs） in a Lyn-PI3K-BTK-dependent manner in vitro. Moreover, ligation of CD180 could also inhibit IFN-α-induced ISG expression in B cells in vivo. Furthermore, the Toll-like receptor 7 and Toll-like receptor 9 signaling pathways could significantly downregulate CD180 expression and modulate the inhibitory effect of CD180 signaling on the activation of I FN-a signaling. Collectively, our results highlight the close association between the increased proportion of CD180-negative B cells and the activation of IFN-α signaling in SLE. Our data provide molecular insight into the mechanism of IFN-α signaling activation in SLE B cells and a potential therapeutic approach for SLE treatment.展开更多
基金support by the National Nature Science Foundation of China (61421002, 61327004)
文摘Phase measuring deflectometry(PMD)is a robust,noncoherent technique for the characterization of specular surface.For measuring high specular reflectivity surface,PMD can deliver micron radian range local gradient.However,when the measured surface has low specular reflectivity,the accuracy of the measured gradient is low since the captured fringe pattern shows low signal to noise ratio.The phase error characteristics in PMD system when testing low reflectivity surfaces are analyzed.The analysis illustrates that the random phase error increases rapidly while the nonlinear error drops slowly with the decreasing of the tested surface reflectivity.In order to attain high precision measurement of low reflectivity specular surface,a robust error reduction method based on wavelet de-noising is proposed to reduce the phase error.This error reduction method is compared with several other normally used methods in both simulation and experiment work.The method based on the wavelet de-noising shows better performance when measuring the low reflectivity specular surface.
基金This work was supported by a grant from National Natural Science Foundation of China （Project number： 31370899）.
文摘A hallmark of systemic lupus erythematosus （SLE） is the consistent production of various auto-antibodies by auto-reactive B cells. Interferon-α（IFN-α） signaling is highly activated in SLE B cells and plays a vital role in the antibody response by B cells. Previous studies have shown that CD180-negative B cells, which are dramatically increased in SLE patients, are responsible for the production of auto-antibodies. However, the association between CD180 and IFN-αsignaling remains unknown. In the present study, we explored the effect of CD180 on regulating the activation of IFN-α signaling in B cells. We found that the number of CD180-negative B cells was increased in MRIJMp-Fas（Ipr/Ipr） lupus-prone mice compared with wild-type mice. Phenotypic analysis showed that CD180-negative B cells comprised CD138＋ plasmablast/plasma cells and GL-7＋ germinal center （GC） B cells. Notably, ligation of CD180 significantly inhibited the I FN-α-induced phosphorylation of signal transducer and activator of transcription 2 （STAT-2） and expression of IFN-stimulated genes （ISGs） in a Lyn-PI3K-BTK-dependent manner in vitro. Moreover, ligation of CD180 could also inhibit IFN-α-induced ISG expression in B cells in vivo. Furthermore, the Toll-like receptor 7 and Toll-like receptor 9 signaling pathways could significantly downregulate CD180 expression and modulate the inhibitory effect of CD180 signaling on the activation of I FN-a signaling. Collectively, our results highlight the close association between the increased proportion of CD180-negative B cells and the activation of IFN-α signaling in SLE. Our data provide molecular insight into the mechanism of IFN-α signaling activation in SLE B cells and a potential therapeutic approach for SLE treatment.
