[Objectives]The paper was better understand the mechanisms of Zn plant uptake in the presence of EDTA and to evaluate the contributions of Zn-EDTA complexes to Zn uptake.[Methods]Three alfalfa cultivars were cultivate...[Objectives]The paper was better understand the mechanisms of Zn plant uptake in the presence of EDTA and to evaluate the contributions of Zn-EDTA complexes to Zn uptake.[Methods]Three alfalfa cultivars were cultivated for 60 d before exposure to 0,250μg Zn and 250μg Zn+10μg EDTA per kg soil for 50 d.Zn concentrations in tissues were analyzed by flame atomic absorbance spectrometry.Subsequently,Zn amount per plant,translocation factor(TF)and bio-concentration factor(BCF)were calculated.Nonenzymatic compounds in tissues were analyzed spectrophotometrically.[Results]Application of Zn+EDTA expressively increased biomass of different tissues of three alfalfa cultivars.Among the three alfalfa cultivars,Medicago sativa ssp.displayed the highest Zn concentration in tissues,the largest Zn amount in aerial parts,and the highest BCF in aerial parts under Zn+EDTA exposure.Under Zn+EDTA stress,increases in free proline in roots,stem,and leaves of M.sativa ssp.were found.Inhibited O_(2)^(·-)production in stem and leaves,increases in soluble sugar,but decreases in soluble protein were observed in M.sativa ssp.[Conclusions]M.sativa ssp.is superior to other two cultivars for Zn phyto-remediation,and its well-coordinated physiological changes under Zn+EDTA exposure confer the great Zn tolerance of this cultivar.展开更多
To evaluate the zinc(Zn)remediation capacity of four alfalfa species,the effects of different concentrations of Zn on plant growth,Zn uptake and translocation as well as uptake of other nutrients were investigated.The...To evaluate the zinc(Zn)remediation capacity of four alfalfa species,the effects of different concentrations of Zn on plant growth,Zn uptake and translocation as well as uptake of other nutrients were investigated.The results showed that the Zn tolerance index of Aohan was significantly higher than other species.Among the four species,Aohan had the highest concentration of Zn in roots,followed by Golden Empress,Sanditi and Longxi.Aohan had the highest bioconcentration factor(BCF)in leaves.Whereas,Sanditi and Longxi had the lowest BCF in stem and roots,respectively.The translocation factor of Golden Empress was significantly lower than other species.The Zn accumulation rate of Aohan was higher than other species regardless of the concentration of Zn.Longxi had the lowest allocation of Zn in leaves and Golden Empress had the lowest allocation of Zn in roots.The concentrations of other elements(Fe and Mg)in leaves were decreased with Zn additions,but the interactions between Zn and other elements in roots varied with species.These results indicated that suitable species of alfalfa could successfully be used for the phytoremediation of Zn-contaminated soils.展开更多
Dear Editor,Remediation of cadmium(Cd)-contaminated soil is a popular topic in global environmental science.Phytoremediation using hyperaccumulators or certain plants to accumulate and remove Cd from polluted soils is...Dear Editor,Remediation of cadmium(Cd)-contaminated soil is a popular topic in global environmental science.Phytoremediation using hyperaccumulators or certain plants to accumulate and remove Cd from polluted soils is a promising approach.Wei et al.(2005)first described Solanum nigrum L.as a Cd hyperaccumulator.Subsequently,various aspects of S.nigrum,including the protective effects on proline,physiological and biochemical responses,earthworms,arbuscular mycorrhizal fungi,and fertilizers and the intercropping with Solanum photeinocarpum,have been extensively studied(Xu et al.,2009;Khan et al.,2014;Wang et al.,2015;Tang et al.,2017;Yang et al.,2019).展开更多
The effects of different electrode on Solanum nigrum L.seed germination were determined.The result showed that germination percentage(GP)of seeds in treatment T2(titanium elec-trode)was 26.6%higher than in control(CK,...The effects of different electrode on Solanum nigrum L.seed germination were determined.The result showed that germination percentage(GP)of seeds in treatment T2(titanium elec-trode)was 26.6%higher than in control(CK,without electric field).High potassium and calcium concentrations were beneficial for seed enzymatic activity in treatment T2,which could partly explain the increase in GP.Cd accumulation(μg/pot)in S.nigrum treated with any electric field was significantly higher(p<0.05)than in CK without electric field.Specif-ically,Cd accumulation under the treatment T3(stainless steel electrode)was the highest both in roots and shoots;this accumulation in shoots and roots were 74.7%and 67.4%higher for stainless steel than in CK.This increase must have been associated with a higher Cd concentration in plants and did not exert a significant effect on the biomass.In par-ticular,Cd concentrations in roots and shoots under stainless steel treatment were both significantly higher than in CK(p<0.05),which had to be related to the higher available Cd concentration in the soil in the middle region.Furthermore,it could be attributed to al-tered soil pH and other soil properties.Moreover,none of the biomasses were significantly affected(p<0.05)by different electrode materials compared to CK.展开更多
基金Supported by National Natural Science Foundation of China(41571300,31270540 and 31070455)Special Plan in the Major Research&Development of the 13th Five-Year Plan of China(2016YFD0800802)+3 种基金Open Fund of Cultivation State Key Laboratory of Qinba Biological Resources and Ecological Environment of Shaanxi University of Technology(SLGPT2019KF04-02)Scientific Research Project of City-University Co-construction of Shaanxi Province(SXJ-2101)Project of Foreign Experts Bureau of Shaanxi Province of China(2022WGZJ-20)Department of Education of Shaanxi Province(15JK1121)。
文摘[Objectives]The paper was better understand the mechanisms of Zn plant uptake in the presence of EDTA and to evaluate the contributions of Zn-EDTA complexes to Zn uptake.[Methods]Three alfalfa cultivars were cultivated for 60 d before exposure to 0,250μg Zn and 250μg Zn+10μg EDTA per kg soil for 50 d.Zn concentrations in tissues were analyzed by flame atomic absorbance spectrometry.Subsequently,Zn amount per plant,translocation factor(TF)and bio-concentration factor(BCF)were calculated.Nonenzymatic compounds in tissues were analyzed spectrophotometrically.[Results]Application of Zn+EDTA expressively increased biomass of different tissues of three alfalfa cultivars.Among the three alfalfa cultivars,Medicago sativa ssp.displayed the highest Zn concentration in tissues,the largest Zn amount in aerial parts,and the highest BCF in aerial parts under Zn+EDTA exposure.Under Zn+EDTA stress,increases in free proline in roots,stem,and leaves of M.sativa ssp.were found.Inhibited O_(2)^(·-)production in stem and leaves,increases in soluble sugar,but decreases in soluble protein were observed in M.sativa ssp.[Conclusions]M.sativa ssp.is superior to other two cultivars for Zn phyto-remediation,and its well-coordinated physiological changes under Zn+EDTA exposure confer the great Zn tolerance of this cultivar.
基金Supported by Natural Science Foundation of Shannxi Province,China(15JK1121,2015JM3086)the Open Fund of Cultivation State Key Laboratory of Qinba Biological Resources and Ecological Environment of Shaanxi University of Technology,China(SLGPT2019KF04-02)+1 种基金Scientific research project of City-University co-construction of Shaanxi Province,China(SXJ-2101)The Project of Foreign Experts Bureau of Shaanxi Province of China(G2021041011L,2022WGZJ-20,G20200241015)。
文摘To evaluate the zinc(Zn)remediation capacity of four alfalfa species,the effects of different concentrations of Zn on plant growth,Zn uptake and translocation as well as uptake of other nutrients were investigated.The results showed that the Zn tolerance index of Aohan was significantly higher than other species.Among the four species,Aohan had the highest concentration of Zn in roots,followed by Golden Empress,Sanditi and Longxi.Aohan had the highest bioconcentration factor(BCF)in leaves.Whereas,Sanditi and Longxi had the lowest BCF in stem and roots,respectively.The translocation factor of Golden Empress was significantly lower than other species.The Zn accumulation rate of Aohan was higher than other species regardless of the concentration of Zn.Longxi had the lowest allocation of Zn in leaves and Golden Empress had the lowest allocation of Zn in roots.The concentrations of other elements(Fe and Mg)in leaves were decreased with Zn additions,but the interactions between Zn and other elements in roots varied with species.These results indicated that suitable species of alfalfa could successfully be used for the phytoremediation of Zn-contaminated soils.
基金supported by Sanqin Talents,Shaanxi Provincial First-class Team——“Contaminated Soil Remediation and Resource Utilization Innovation Team at Shaanxi University of Technology”,China,Shaanxi University of Technology Research Ability Enhancement Project,China(No.SLGNL202405)Qin Chuangyuan“Scientists+Engineers”Team Construction in Shaanxi Province,China(No.2024QCY-KXJ-104)+5 种基金the Open Fund of Cultivation State Key Laboratory of Qinba Biological Resources and Ecological Environment,China(No.SLGPT2019KF0402)Qinba Bioremediation and Resource Development Research and Innovation Base,China(No.2022-ZC-GXYZ0029)the Project of Foreign Experts Bureau of Shaanxi Province,China(No.2024WZ-ZDXM-15)the Project of Foreign Experts Bureau of China(Nos.G2023041029L and DL2023041006L)the City University Co-construction of Shaanxi Province,China(No.SXJ-2101)the program financed by Poland Minister of Science under the“Regional Excellence Initiative”for 2024–2027。
文摘Dear Editor,Remediation of cadmium(Cd)-contaminated soil is a popular topic in global environmental science.Phytoremediation using hyperaccumulators or certain plants to accumulate and remove Cd from polluted soils is a promising approach.Wei et al.(2005)first described Solanum nigrum L.as a Cd hyperaccumulator.Subsequently,various aspects of S.nigrum,including the protective effects on proline,physiological and biochemical responses,earthworms,arbuscular mycorrhizal fungi,and fertilizers and the intercropping with Solanum photeinocarpum,have been extensively studied(Xu et al.,2009;Khan et al.,2014;Wang et al.,2015;Tang et al.,2017;Yang et al.,2019).
基金This work was supported by the National Natural Science Foundation of China(Nos.31870488,41571300)the Open Fund of Cultivation State Key Laboratory of Qinba Biological Resources and Ecological Environment of Shaanxi University of Technology(No.SLGPT2019KF04-02)the project of Foreign Experts Bureau of Shaanxi province of China(No.G20200241015).
文摘The effects of different electrode on Solanum nigrum L.seed germination were determined.The result showed that germination percentage(GP)of seeds in treatment T2(titanium elec-trode)was 26.6%higher than in control(CK,without electric field).High potassium and calcium concentrations were beneficial for seed enzymatic activity in treatment T2,which could partly explain the increase in GP.Cd accumulation(μg/pot)in S.nigrum treated with any electric field was significantly higher(p<0.05)than in CK without electric field.Specif-ically,Cd accumulation under the treatment T3(stainless steel electrode)was the highest both in roots and shoots;this accumulation in shoots and roots were 74.7%and 67.4%higher for stainless steel than in CK.This increase must have been associated with a higher Cd concentration in plants and did not exert a significant effect on the biomass.In par-ticular,Cd concentrations in roots and shoots under stainless steel treatment were both significantly higher than in CK(p<0.05),which had to be related to the higher available Cd concentration in the soil in the middle region.Furthermore,it could be attributed to al-tered soil pH and other soil properties.Moreover,none of the biomasses were significantly affected(p<0.05)by different electrode materials compared to CK.